Whole-Genome Sequence of
            <i>Mycoplasma bovis</i>
            Strain Ningxia-1

Sun, Peng; Luo, Haifeng; Zhang, Xin; Xu, Jingyi; Guo, Yanan; He, Shenghu

doi:10.1128/genomea.01367-17

Cited by 10 publications

(5 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The first strain (40) clustered with M. bovis strains: 08M, Ningxia-1 and JF4278. The first two of these strains have been isolated in China [30,31], while the third strain was identified in Switzerland [3]. The second field strain of M. bovis in this study (9029) was grouped with Egyptian and Hungarian strains [32,33].…”

Section: Discussionmentioning

confidence: 81%

Phylogenetic relationship among field isolates of mycoplasmas and acholeplasmas in two South Australian dairy herds based on sequencing of a short 16S rRNA gene fragment

Al-Farha¹

2020

Proceedings of the Proceedings of the 1st International Multi-Disciplinary Conference Theme: Sustainable Development and Smart

View full text Add to dashboard Cite

Mycoplasma mastitis has been recognized as an emerging disease with a significant impact on the dairy industry. This study aimed to determine the genotypic distribution of some Mycoplasma and Acoleplasma spp isolated from two commercial dairy farms in South Australia based on 16S rRNA sequencing, and compare their evolutionary relationship to some similar isolates from elsewhere. Neighbour-joining phylogeny of 16S rRNA demonstrated the involvement of four different spp. isolated from Farm 1 including: M. bovis, M. bovirhinis, Acholeplasma laidlawii and A. axanthum while M. alkalescens was isolated from Farm 2. Two strains of M. bovis showed similarity to Egyptian, Hungarian and Chinese strains. M. bovirhinis clustered with the Egyptian group. Mycoplasma alkalescens grouped with Swedish and Japanese strains. The Acholeplasma group showed two distinct clusters of A. laidlawii and A. axanthum. Determination of the species/genera involved in mastitis may enhance the molecular epidemiology and in-turn can contribute to controlling strategies of the disease.

show abstract

Section: Discussionmentioning

confidence: 81%

Phylogenetic relationship among field isolates of mycoplasmas and acholeplasmas in two South Australian dairy herds based on sequencing of a short 16S rRNA gene fragment

Al-Farha¹

2020

Proceedings of the Proceedings of the 1st International Multi-Disciplinary Conference Theme: Sustainable Development and Smart

View full text Add to dashboard Cite

show abstract

“…Subsequently, clustering was performed using ppanggolin cluster, followed by the graph construction and partitioning (ppanggolin graph, ppanggolin partition). After generating our 36 assemblies, we compared our data with the study by Yair et al (27), which included 175 assemblies (whole-genome shotgun project PRJNA564939), as well as seven NCBI RefSeq genomes of M. bovis (PG45 NC_014760.1, Hubei-1 NC_015725.1, HB0801 NC_018077.1, CQ-W70 NZ_CP005933.1, 08M NZ_CP019639.1, Ningxia-1 NZ_CP023663.1, JF4278 NZ_LT578453.1) (28)(29)(30)(31)(32) and one genome provided by the NCBI (NM2012 CP011348.1).…”

Section: Genome Synteny Analysismentioning

confidence: 99%

De novogenome assembly resolving repetitive structures enables genomic analysis of 35 EuropeanMycoplasma bovisstrains

Triebel

Sachse

Weber

et al. 2023

Preprint

View full text Add to dashboard Cite

Mycoplasma (M.) bovis, the agent of mastitis, pneumonia, and arthritis in cattle, harbors a small genome of approximately 1 Mbp. Combining data from Illumina and Nanopore technologies, we sequenced and assembled the genomes of 35 European strains and isolate DL422_88 from Cuba. While the high proportion of repetitive structures in M. bovis genomes represents a particular challenge, implementation of our own pipeline Mycovista (available on GitHub www.github.com/sandraTriebel/mycovista) in a hybrid approach enabled contiguous assembly of the genomes and, consequently, improved annotation rates considerably. To put our European strain panel in a global context, we analyzed the new genome sequences together with 175 genome assemblies from public databases. Construction of a phylogenetic tree based on core genes of these 219 strains revealed a clustering pattern according to geographical origin, with European isolates positioned on clades 4 and 5. Genomic data allowing assignment of strains to tissue specificity or certain disease manifestations could not be identified. Seven strains isolated from cattle with systemic circular condition (SCC), still a largely unknown manifestation of M. bovis disease, were located on both clades 4 and 5. Pairwise association analysis revealed 108 genomic elements associated with a particular clade of the phylogenetic tree. Further analyzing these hits, 25 genes are functionally annotated and could be linked to a M. bovis protein, e.g. various proteases and nucleases, as well as ten variable surface lipoproteins (Vsps) and other surface proteins. These clade-specific genes could serve as useful markers in epidemiological and clinical surveys.

show abstract

“…The availability of completely sequenced genomes of S. auerus allows elucidation of amino acid sequences and identification of dominant epitopes of newly discovered mastitogenic pathogens virulence factors to be used in the design of vaccines for better control of mastitis (Baba et al, 2008). The complete genome sequences are available for 5 Mycoplasma bovis isolates (Sun et al, 2018). Comparing the genetic diversity between 82 Mycoplasma bovis isolates which were collected from various geographical locations by using WGS.…”

Section: Whole Genome Sequencingmentioning

confidence: 99%

Comparative Diagnosis of Infectious Bacteria in Bovine Milk

Sedky¹,

Ghazy²,

Soliman³

et al. 2020

JAHP

View full text Add to dashboard Cite

B ovine milk is a source of nutrition for people and calf since it contains the supplements necessary for growth and progress of the calf. It could be a source of lipids, proteins, amino acids, minerals and vitamins. It contains immunoglobulins, hormones, growth factors, cytokines, nucleotides and peptides (Haug et al., 2007). Raw bovine milk is considered as a suitable growth medium for the multiplication of nearly all kinds of microorganisms (Kumar et al., 2010; Mahmoud et al., 2019). Bovine milk is considered as a vehicle for many infectious bacterial diseases. The most common infectious bacterial diseases that spread via bovine milk to young calves and human are bovine tuberculosis, brucellosis, mycoplasma infection, leptospirosis, and Q fever.

show abstract

Whole-Genome Sequence of Mycoplasma bovis Strain Ningxia-1

Cited by 10 publications

References 7 publications

Phylogenetic relationship among field isolates of mycoplasmas and acholeplasmas in two South Australian dairy herds based on sequencing of a short 16S rRNA gene fragment

Phylogenetic relationship among field isolates of mycoplasmas and acholeplasmas in two South Australian dairy herds based on sequencing of a short 16S rRNA gene fragment

De novogenome assembly resolving repetitive structures enables genomic analysis of 35 EuropeanMycoplasma bovisstrains

Comparative Diagnosis of Infectious Bacteria in Bovine Milk

Contact Info

Product

Resources

About