Whole-Genome Sequence of
            <i>Monascus purpureus</i>
            GB-01, an Industrial Strain for Food Colorant Production

Kumagai, Toshitaka; Tsukahara, Masatoshi; Katayama, Naoya; Yaoi, Katsuro; Aburatani, Sachiyo; Ohdan, Kohji; Fujimori, Kazuhiro E.

doi:10.1128/mra.00196-19

Cited by 6 publications

(6 citation statements)

References 10 publications

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“…Based on the LOV and MK BGCs in Table 3 , we compared twelve genomes of Monascus spp. including eight published genomes, M. purpureus YY-1, YJX-8, GB-01, HQ1, NRRL1596, and M. ruber FWB13, CBS127566, NRRL 1597 [ 29 , 30 , 31 , 32 ], and four genomes of M. pilosus sequenced in this study, and found that all genomes of four M. pilosus strains and three M. ruber strains contained the MK BGCs while there was no MK BGC in those of five M. purpureus strains. Total 9 or 10 genes named mkA-mkI were highly conserved in the LOV and MK BGCs ( Table 4 , Figure 4 ).…”

Section: Resultsmentioning

confidence: 99%

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Production of Monacolin K in Monascus pilosus: Comparison between Industrial Strains and Analysis of Its Gene Clusters

2021

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Monascus pilosus strains are widely applied to yield a cholesterol synthesis inhibitor monacolin K (MK), also called lovastatin (LOV). However, the mechanism of MK production by M. pilosus strains is still unclear. In this study, we firstly confirmed four Monascus strains, MS-1, YDJ-1, YDJ-2, and K104061, isolated from commercial MK products as M. pilosus and compared their abilities to produce MK in solid-state and liquid-state cultures. Then, we sequenced and analyzed their genomes and MK biosynthetic gene clusters (BGCs). The results revealed that the MK yields of MS-1, YDJ-1, YDJ-2, and K104061 in solid-state cultures at 14 days were 6.13, 2.03, 1.72, and 0.76 mg/g, respectively; the intracellular and extracellular MK contents of MS-1, YDJ-1, YDJ-2, and K104061 in liquid-state cultures at 14 days reached 0.9 and 1.8 mg/g, 0.38 and 0.43 mg/g, 0.30 and 0.42 mg/g, and 0.31 and 0.76 mg/g, respectively. The genome sizes of the four M. pilosus strains were about 26 Mb, containing about 7000–8000 coding genes and one MK gene cluster. The MK BGCs of MS-1, YDJ-2, and K104061 contained 11 genes, and the MK BGC of YDJ-1 contained 9 genes. According to the literature search, there are few comparisons of gene clusters and related genes responsible for the synthesis of LOV and MK. We also compared the LOV BGC in A. terreus with the MK BGCs in different species of Monascus spp., and the results revealed that although LOV and MK were the same substance, the genes responsible for the synthesis of MK were much less than those for LOV synthesis, and the gene functions were quite different. The current results laid a foundation to explore the mechanism of MK produced by Monascus spp. and compare the synthesis of LOV and MK.

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Section: Resultsmentioning

confidence: 99%

“…Up to now, no genome information of M. pilosus has been released, and few industrially applied strains of Monascus spp. are available on the genome [ 29 , 30 , 31 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

Production of Monacolin K in Monascus pilosus: Comparison between Industrial Strains and Analysis of Its Gene Clusters

2021

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“…The omics-based technologies comprising genomics ( 29 , 30 ), transcriptomics ( 30 – 34 ), and proteomics ( 34 ) have been used to identify metabolic mechanisms implicated in carbon starvation stress ( 30 ), exposure to blue light ( 35 ), gene knockout mutants of mga 1–3 ( 32 ), and transmembrane secretion of extracellular MPs ( 34 ) in Monascus strains. These approaches help to elucidate the regulatory mechanisms of MP synthesis and diversity.…”

Section: Introductionmentioning

confidence: 99%

Integrative Metabolomic and Transcriptomic Analyses Uncover Metabolic Alterations and Pigment Diversity in Monascus in Response to Different Nitrogen Sources

et al. 2021

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“…Kumagai et al reported the genome sequence information of the high pigment-producing M . purpureus GB-01 strain, with a genome size of 24.3 Mb and 121 chromosomal contigs [ 20 ]. Liu et al identified the key genes ( ERG4 A and ERG4 B) for ergosterol biosynthesis in M .…”

Section: Introductionmentioning

confidence: 99%

Analysis of secondary metabolite gene clusters and chitin biosynthesis pathways of Monascus purpureus with high production of pigment and citrinin based on whole-genome sequencing

et al. 2022

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Monascus is a filamentous fungus that is widely used for producing Monascus pigments in the food industry in Southeast Asia. While the development of bioinformatics has helped elucidate the molecular mechanism underlying metabolic engineering of secondary metabolite biosynthesis, the biological information on the metabolic engineering of the morphology of Monascus remains unclear. In this study, the whole genome of M. purpureus CSU-M183 strain was sequenced using combined single-molecule real-time DNA sequencing and next-generation sequencing platforms. The length of the genome assembly was 23.75 Mb in size with a GC content of 49.13%, 69 genomic contigs and encoded 7305 putative predicted genes. In addition, we identified the secondary metabolite biosynthetic gene clusters and the chitin synthesis pathway in the genome of the high pigment-producing M. purpureus CSU-M183 strain. Furthermore, it is shown that the expression levels of most Monascus pigment and citrinin clusters located genes were significantly enhanced via atmospheric room temperature plasma mutagenesis. The results provide a basis for understanding the secondary metabolite biosynthesis, and constructing the metabolic engineering of the morphology of Monascus.

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Whole-Genome Sequence of Monascus purpureus GB-01, an Industrial Strain for Food Colorant Production

Cited by 6 publications

References 10 publications

Production of Monacolin K in Monascus pilosus: Comparison between Industrial Strains and Analysis of Its Gene Clusters

Production of Monacolin K in Monascus pilosus: Comparison between Industrial Strains and Analysis of Its Gene Clusters

Integrative Metabolomic and Transcriptomic Analyses Uncover Metabolic Alterations and Pigment Diversity in Monascus in Response to Different Nitrogen Sources

Analysis of secondary metabolite gene clusters and chitin biosynthesis pathways of Monascus purpureus with high production of pigment and citrinin based on whole-genome sequencing

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