Whole genome sequence and genome annotation of Colletotrichum acutatum , causal agent of anthracnose in pepper plants in South Korea

Abstract

Colletotrichum acutatum is a destructive fungal pathogen which causes anthracnose in a wide range of crops. Here we report the whole genome sequence and annotation of C. acutatum strain KC05, isolated from an infected pepper in Kangwon, South Korea. Genomic DNA from the KC05 strain was used for the whole genome sequencing using a PacBio sequencer and the MiSeq system. The KC05 genome was determined to be 52,190,760 bp in size with a G + C content of 51.73% in 27 scaffolds and to contain 13,559 genes with an average length of 1516 bp. Gene prediction and annotation were performed by incorporating RNA-Seq data. The genome sequence of the KC05 was deposited at DDBJ/ENA/GenBank under the accession number LUXP00000000.

Optimization of Polyethylene Glycol-Mediated Transformation of the Pepper Anthracnose Pathogen Colletotrichum scovillei to Develop an Applied Genomics Approach

“…The previously identified strain C . acutatum KC05 (Han et al, 2016) was used in the present study to confirm the identity in C . acutatum sensu lato.…”

“…Colletotrichum acutatum KC05 isolated from an infected pepper fruit in Gangwon Province of South Korea was used in this study (Han et al, 2016). The isolate was identified based on combined sequence analyses of the nuclear ribosomal internal transcribed (ITS) region, β-tubulin (TUB2), actin (ACT), partial sequences of the chitin synthase 1 (CHS-1), an intron sequence of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and histone 3 (HIS3) genes (Damm et al, 2012; da Silva et al, 2017).…”

“…The six gene sequences of C . acutatum KC05 were obtained from Han et al (2016), and the gene sequences of other Colletotrichum species ( C . acutatum , C .…”

“…Nineteen loci were selected from 10 of 27 different scaffolds (Han et al, 2016). For double-joint PCR and Southern blots, genomic DNA was extracted using a quick DNA extraction method (Chi et al, 2009).…”

“…capsici , infect pepper (Bailey and Jeger, 1992; Than et al, 2008). Colletotrichum acutatum is one of the most frequently reported species causing anthracnose disease on pepper in tropical and subtropical countries (Han et al, 2016). Using combined sequence analyses of the β-tublin-2 (TUB2) gene, the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene, and internal transcribed spacer (ITS) rDNA regions, C .…”