Whole genome sequence analysis of Cupriavidus campinensis S14E4C, a heavy metal resistant bacterium

Abstract

Cupriavidus sp. are model organisms for heavy metal(loid) resistance and aromatic compound's degradation studies and these characteristics make them a perfect candidate for biotechnological purposes. Bacterial strain S14E4C (identified as Cupriavidus campinensis) was isolated from a playground by enrichment method in a 0.25 mM containing medium. The analysis revealed that this bacterium is able to tolerate high concentrations of heavy metal(loid)s: Cd up to 19.5 mM, Pb to 9 mM, Hg to 5.5 mM and As to 2 mM in heavy metal(loid) salt containing nutrient medium. The whole genome data and analysis of the type strain of C. campinensis CCUG:44526 T have not been available so far, thus here we present the genome sequencing results of strain S14E4C of the same species. Analysis was carried out to identify possible mechanisms for the heavy metal resistance and to map the genetic data of C. campinensis. The annotation pipelines revealed that the total genome of strain S14E4C is 6,375,175 bp length with a GC content of 66.3% and contains 2 plasmids with 295,460 bp (GC content 59.9%) and 50,483 bp (GC content 63%). In total 4460 coding sequences were assigned to known functions and 1508 to hypothetical proteins. Analysis proved that strain S14E4C is having gene clusters such as czc, mer, cus, chr, ars to encode various heavy metal resistance mechanisms that play an important role to survive in extreme environments.

Hospital sink traps as a potential source of the emerging multidrug-resistant pathogen Cupriavidus pauculus: characterization and draft genome sequence of strain MF1

“…gilardii [80], C. campinensis [81] and C. neocaledonicus [82] are considered to be metal resistant. Many of these genes confer resistance to metals by means of intracellular metal sequestration to facilitate export, as in the case of CopZ with Cu + ions [83], Ag + and Cd 2+ ions [84].…”

“…Together, the MRGs listed in Table 1 contribute to resistance to copper, silver, magnesium, cobalt, mercury, molybdenum, arsenic, zinc, cadmium, iron, antimony and nickel. Many species of Cupriavidus including C. pauculus [ 76, 77 ], C. metallidurans [ 78, 79 ], C. gilardii [ 80 ], C. campinensis [ 81 ] and C. neocaledonicus [ 82 ] are considered to be metal resistant. Many of these genes confer resistance to metals by means of intracellular metal sequestration to facilitate export, as in the case of CopZ with Cu + ions [ 83 ], Ag + and Cd 2+ ions [ 84 ].…”

The diverse functional genes of maize rhizosphere microbiota assessed using shotgun metagenomics

“…Other notable genes mined from our metadata include drrABC, daunorubicin resistance genes 37 ; phzEFH, phenazine biosynthesis protein (biocontrol); 38 fepBCDEG, siderophore enterobactin or ferric enterobactin transport ATP-binding protein; 39 czcC, heavy metal efflux protein to resist the effect of cobalt, zinc and cadmium 40 ; soxBXYZ, sulfur oxidizing multi-enzyme genes 41 ; trehalose synthase, uptake of exogenous trehalose 42 and cysteine desulfurase family (sufs, iscs and csdA). 43…”

Cadmium Bioremediation Potential of Bacillus sp. and Cupriavidus sp.

“…HMR-1 isolated from waste water proves it to be having resistance towards multiple heavy metals especially cadmium 25 . Additionally, one more finding of Cupriavidus compinesis S14E4C exhibited its ability to tolerate cadmium upto 19.5mM 26 . Genomic screening of Bacillus cereus NWUAB01 revealed that it can tolerate 200 mg/L of Cd 27 .…”