Whole-exome sequencing and genome-wide evolutionary analyses identify novel candidate genes associated with infrared perception in pit vipers

Abstract

pit vipers possess a unique thermal sensory system consisting of facial pits that allow them to detect minute temperature fluctuations within their environments. Biologists have long attempted to elucidate the genetic basis underlying the infrared perception of pit vipers. Early studies have shown that the TRPA1 gene is the thermal sensor associated with infrared detection in pit vipers. However, whether genes other than TRPA1 are also involved in the infrared perception of pit vipers remains unknown. Here, we sequenced the whole exomes of ten snake species and performed genome-wide evolutionary analyses to search for novel candidate genes that might be involved in the infrared perception of pit vipers. We applied both branch-length-comparison and selection-pressure-alteration analyses to identify genes that specifically underwent accelerated evolution in the ancestral lineage of pit vipers. A total of 47 genes were identified. These genes were significantly enriched in the ion transmembrane transporter, stabilization of membrane potential, and temperature gating activity functional categories. The expression levels of these candidate genes in relevant nerve tissues (trigeminal ganglion, dorsal root ganglion, midbrain, and cerebrum) were also investigated in this study. We further chose one of our candidate genes, the potassium channel gene KCNK4, as an example to discuss its possible role in the infrared perception of pit vipers. Our study provides the first genome-wide survey of infrared perception-related genes in pit vipers via comparative evolutionary analyses and reveals valuable candidate genes for future functional studies. Infrared perception is a notable evolutionary adaptation of snakes that helps snakes respond to the external environment during their nocturnal activities and prey on warm-blooded animals 1-3. Pit vipers are the best at sensing and utilizing infrared signals among all snakes 4-6. They have the most sensitive infrared sensory system, which can detect minute temperature fluctuations as low as 0.001 °C in their immediate surroundings 7. This peculiar infrared perception ability enables pit vipers to detect and locate warm-blooded animals with high precision in the dark, which makes them one of the most successful predators in nature 8-10. Biologists have long sought to investigate the biological basis of infrared perception in pit vipers. Previous anatomical and neurophysiological studies have accumulated substantial data explaining the detection and transduction mechanism of pit viper infrared perception 6,11-17. We now know that the infrared perception of pit vipers is initiated by the heat stimulation of a thin sensory membrane embedded in the facial loreal pit, a highly specialized organ located between the eye and nostril 11,12. The sensory membrane is innervated by afferent fibres of trigeminal nerve branches that transduce infrared signals to a particular nucleus in the hindbrain 13-15. The efferents of the nucleus then project to the optic tectum, where infrared information is int...

First Genome of Rock Lizard Darevskia valentini Involved in Formation of Several Parthenogenetic Species

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Embryonic development of the neotropical pit viper Bothrops atrox (Serpentes: Viperidae: Crotalinae), with emphasis on pit organ morphogenesis and its evolution in snakes

“…37 Some similarities in neuroanatomy, physiology, and genetics have been found in the pit organs of boas, pythons, and pit vipers. 23,51,53,55 These organs are innervated by somatosensory nerve fibers originating from the maxillary branch of the trigeminus nerve. 23,51 These nerve terminals indirectly detected IR radiation by triggering the activation of the thermoreceptor molecule TRPA1, caused by the heating of the tissues within the pit.…”

“…23,51 These nerve terminals indirectly detected IR radiation by triggering the activation of the thermoreceptor molecule TRPA1, caused by the heating of the tissues within the pit. 54,55 TRPA1 is a calcium ion channel protein identified as the IR receptor in snakes with pit organs. 54,55 The pit organs of snakes have been compared using adult specimens, but further research on their ontogeny is still needed.…”

“…54,55 TRPA1 is a calcium ion channel protein identified as the IR receptor in snakes with pit organs. 54,55 The pit organs of snakes have been compared using adult specimens, but further research on their ontogeny is still needed.…”