Whole Cells in Enantioselective Reduction of<i>tert</i>-Butyl Acetoacetate

Ramos, Aline de Souza; Ribeiro, Joyce Benzaquem; Lopes, Raquel de Oliveira; Souza, Rodrigo O. M. A. de

doi:10.1080/00397911.2012.655685

Cited by 5 publications

(1 citation statement)

References 29 publications

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“…In our study of effective reduction of (±)-α-acetyl-γ-butyrolactone 1 , we chose Rhodotorula strains, because of their known ability to commonly reduce low-molecular-weight ketones—derivatives of acetophenone [5,6,7,8,9]—and ketoesters [10]. …”

Section: Introductionmentioning

confidence: 99%

Biotransformation of α-Acetylbutyrolactone in Rhodotorula Strains

Mączka

Wińska

Grabarczyk

et al. 2018

IJMS

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Due to its structural similarity, the α’-1′-hydroxyethyl-γ-butyrolactone obtained by reduction of (±)-α-acetyl-γ-butyrolactone may have a similar function in the body to γ-butyrolactone (GBL). In the work presented, biotransformation of α-acetyl-γ-butyrolactone by three Rhodotorula strains was performed obtaining enantiomerically enriched alcohol. The process was carried out in growing and resting cultures. We studied how both media composition and organic solvent volume affected stereoselectivity and effectiveness of biotransformation. After 2 h, the enantiomerically pure (3R, 1′S)-α’-1′-hydroxyethyl-γ-butyrolactone was obtained using the R. marina AM77 strain in YPG (Yeast extract-Peptone-Glucose) medium enriched with 5% glycerol. To our best knowledge there is no previous information in the literature about the (±)-α-acetyl-γ-butyrolactone biotransformation performed in medium with addition of organic and deep eutectic solvents.

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Section: Introductionmentioning

confidence: 99%

Biotransformation of α-Acetylbutyrolactone in Rhodotorula Strains

Mączka

Wińska

Grabarczyk

et al. 2018

IJMS

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ChemInform Abstract: Whole Cells in Enantioselective Reduction of tert‐Butyl Acetoacetate.

Ramos¹,

Ribeiro²,

Lopes³

et al. 2013

ChemInform

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Whole Cells in Enantioselective Reduction of tert-Butyl Acetoacetate. -Several microorganism strains are tested in the enantioselective reduction of tert-butyl acetoacetate. The best results are obtained with yeast (Rhodorotula rubra) which can be reused three times without loss of enantioselectivity. Similar results are also obtained if these microorganisms are used after immobilization in calcium alginate spheres. -(RAMOS, A. D. S.; RIBEIRO*, J. B.; LOPES, R. D. O.; DE SOUZA, R. O. M. A.; Synth. Commun. 43 (2013) 12, 1611-1618, http://dx.

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