Whole-Body Radioprotective Effects of SBL-1: A Preparation From Leaves ofHippophae rhamnoides

Abstract

The radioprotective effects of Hippophae rhamnoides (common name, sea buckthorn) leaf extract, designated SBL-1, were investigated in Swiss Albino strain 'A' mice. Against 100% mortality in whole-body irradiated ( 60 Co-gamma-rays, 10 Gy) controls, a single dose of the SBL-1 rendered >90% survivors when administered 30 min before irradiation and 90% to 80% survivors when administered 1 to 4 h before irradiation. SBL-1 activated proliferation of hemopoietic stem cells countered a radiation-induced decrease in total thiols and an increase in free radicals in plasma and liver; inhibited lipid peroxidation, and normalized the liver alkaline phosphatase activity. This study demonstrated high radioprotective potential of H. rhamnoides leaves.

In vitroandin silicointeraction of porcine α-amylase withVicia fabacrude seed extract and evaluation of antidiabetic activity

“…Based on literature [18][19][20] the active constituents of Vicia faba seed extract i.e., tannins (Gallic acid, Proanthocyanidins, Catechin, Epicatechin) were selected. Acarbose was selected as a standard drug as reference molecule.…”

Exploring in silico affinity of flavonoids and tannins to human fibroblast growth factor-inducible14 (Fn14), a member of TNF receptor super family

“…Based on our previous report [6, 7] the active constituents of leaf extract from Hippophae rhamnoides i.e., flavonoids (Quercitin, Rutin and Genistein) and tannins (Gallic acid and Ellagic acids), were selected. 3D structures of Ellagic Acid (CID: 5281855), Genistein(CID:5280961), Rutin (CID:5280805) & Gallic acid ethyl ester (CID:13250) and Quercetin (CID:5280343) were downloaded from Pubchem (http:// pubchem.ncbi.nlm.nih.…”

“…On the other hand increased expressions of TWEAK and Fn14 were reported in case of massive liver injury and were associated with uncontrolled proliferation of oval cells or situations associated with hepatocellular carcinoma [5]. We also reported that adverse effects of radiation were prevented by treating the mice with tannins and flavonoids rich extract of Hippophae rhamnoides before irradiation [6, 7], which presumably acted via inhibiting the Fn14-TWEAK interaction [1]. …”

Normal Tissue Protectors Against Radiation Injury

“…It protected DNA in vitro 58,59 , reduced bone marrow cytogenetic damage 60 , scavenged radiation and chemical-induced free radicals 55 , reduced cytotoxicity in cultured cells 61 . Another extract prepared from the leaves of H. rhamnoides, has been reported to be less toxic besides being radioprotective [62][63][64] . It protected genomic DNA at very low concentrations as well as effectively scavenged free radicals 62 .…”

“…Another extract prepared from the leaves of H. rhamnoides, has been reported to be less toxic besides being radioprotective [62][63][64] . It protected genomic DNA at very low concentrations as well as effectively scavenged free radicals 62 . Leaf extract of H. rhamnoides has been reported to protect the liver and hemopoietic system in whole-body irradiated mice 63,64 .…”