Whole body exposure to low frequency magnetic field: No provable effects on the cellular energetics of rat skeletal muscle

Abstract

On the basis of previous experience with biological effects of electromagnetic fields a potential effect of homogeneous sinusoidal magnetic field (50 Hz, 10 mT) on energy state of rat skeletal muscle was investigated. Two different total body exposures to magnetic field were selected: (1) repeated 1 hour exposure, 2 times a week for 3 months, and (2) acute 1.5 hour exposure (and the appropriate control groups). Important energy metabolites (adenosine triphosphate--ATP, creatine phosphate, creatine, lactate, pyruvate and inorganic phosphate) were analysed by enzymatic and spectroscopic methods in musculus gracilis cranialis. On the basis of the concentration of important energy metabolites the apparent Gibbs free energy of ATP hydrolysis and creatine charge was calculated. Our results demonstrate no influence of this low frequency magnetic field on the level of important energy metabolites in rat skeletal muscle. The conclusion of this study is that neither repeated exposure nor the acute exposure of rats to the sinusoidal magnetic field of given parameters has any important influence on the energy state of the skeletal muscle.

Effects of electromagnetic fields of low frequency and low intensity on rat metabolism

“…Exposure to artificially generated electromagnetic (e.m.) fields is a common occurrence for a large number of individuals: its biological consequences, still largely unknown, are being studied in experimental animals [ 1 - 4 ] and in humans [ 5 ]. At present, the body functioning is generally formulated in terms of biomolecules and their interactions; the living state is described in terms of molecular biology.…”

Effects of extremely low-frequency electromagnetic field exposure on the skeletal muscle functions in rats

“…In addition, the isometric contraction forces obtained by different stimulating frequencies (10, 20, 50 and 100 Hz) showed a significant linear increase in the tetanic contraction. On the other hand, Stefl et al (2006) investigated a potential effect of homogeneous sinusoidal MF (50 Hz, 10 mT) on bioenergetics (ATP, creatine phosphate, creatine, lactate, pyruvate and inorganic phosphate) of rat skeletal muscle and they reported that neither repeated exposure nor the acute exposure of rats to the sinusoidal MF has any important influence on the level of important energy metabolites in rat skeletal muscle.…”

Complex Rehabilitation Project in Leigh Syndrome

“…The bio-resonance is the form of resonance that occurs at the level of cell membranes using electromagnetic fields at very low intensity and a specific frequency (cyclotron) which is able to influence and stimulate the metabolism of human cells. It regulates the ordered ionselective traffic between the internal and external environment of the cell and stimulates the activity of metal-active enzymes involved in many biological reactions [35][36][37][38].…”