Whole-body connectome of a segmented annelid larva

Verasztó, Csaba; Jasek, Sanja; Gühmann, Martin; Bezares-Calderón, Luis Alberto; Williams, Elizabeth A.; Shahidi, Réza; Jékely, Gáspár

doi:10.1101/2024.03.17.585258

Cited by 5 publications

(1 citation statement)

References 71 publications

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“…The sensory and neuronal bases of light-guided (Gühmann et al, 2015; Randel et al, 2014; Verasztó et al, 2018) and mechanically-driven behaviours (Bezares-Calderón et al, 2018) in Platynereis larvae have been dissected due to the experimental tractability of this system. Its small size has allowed the entire reconstruction of the cellular and synaptic wiring map of the three-day-old larva (Jasek et al, 2022; Jékely et al, 2024). Here we study Platynereis larvae to understand the cellular and neuronal bases of pressure sensation in zooplankton.…”

Section: Introductionmentioning

confidence: 99%

Mechanism of barotaxis in marine zooplankton

Bezares-Calderón

Shahidi

Jékely

2023

Preprint

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Hydrostatic pressure is a dominant environmental cue for vertically migrating marine organisms but the physiological mechanisms of responding to pressure changes remain unclear. Here we uncovered the cellular and circuit bases of a barokinetic response in the planktonic larva of the marine annelidPlatynereis dumerilii. Increases in pressure induced a rapid, graded and adapting upward swimming response due to faster ciliary beating. By calcium imaging, we found that brain ciliary photoreceptors showed a graded response to pressure changes. The photoreceptors in animals mutant forciliary opsin-1had a smaller ciliary compartment and mutant larvae showed diminished pressure responses. The ciliary photoreceptors synaptically connect to the head multiciliary band that propels swimming via serotonergic motoneurons. Genetic inhibition of the serotonergic cells blocked pressure-dependent increases in ciliary beating. We conclude that ciliary photoreceptors function as pressure sensors and activate ciliary beating through serotonergic signalling during barokinesis.

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Section: Introductionmentioning

confidence: 99%

Mechanism of barotaxis in marine zooplankton

Bezares-Calderón

Shahidi

Jékely

2023

Preprint

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Hierarchical communities in the larval Drosophila connectome: Links to cellular annotations and network topology

Betzel,

Puxeddu,

Seguin

2024

Proc. Natl. Acad. Sci. U.S.A.

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One of the longstanding aims of network neuroscience is to link a connectome’s topological properties—i.e., features defined from connectivity alone–with an organism’s neurobiology. One approach for doing so is to compare connectome properties with annotational maps. This type of analysis is popular at the meso-/macroscale, but is less common at the nano-scale, owing to a paucity of neuron-level connectome data. However, recent methodological advances have made possible the reconstruction of whole-brain connectomes at single-neuron resolution for a select set of organisms. These include the fruit fly, Drosophila melanogaster , and its developing larvae. In addition to fine-scale descriptions of connectivity, these datasets are accompanied by rich annotations. Here, we use a variant of the stochastic blockmodel to detect multilevel communities in the larval Drosophila connectome. We find that communities partition neurons based on function and cell type and that most interact assortatively, reflecting the principle of functional segregation. However, a small number of communities interact nonassortatively, forming form a “rich-club” of interneurons that receive sensory/ascending inputs and deliver outputs along descending pathways. Next, we investigate the role of community structure in shaping communication patterns. We find that polysynaptic signaling follows specific trajectories across modular hierarchies, with interneurons playing a key role in mediating communication routes between modules and hierarchical scales. Our work suggests a relationship between system-level architecture and the biological function and classification of individual neurons. We envision our study as an important step toward bridging the gap between complex systems and neurobiological lines of investigation in brain sciences.

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Mechanism of barotaxis in marine zooplankton

Bezares Calderón,

Shahidi,

Jékely

2024

eLife

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Hydrostatic pressure is a dominant environmental cue for vertically migrating marine organisms but the physiological mechanisms of responding to pressure changes remain unclear. Here we uncovered the cellular and circuit bases of a barokinetic response in the planktonic larva of the marine annelid Platynereis dumerilii . Increases in pressure induced a rapid, graded and adapting upward swimming response due to faster ciliary beating. By calcium imaging, we found that brain ciliary photoreceptors showed a graded response to pressure changes. The photoreceptors in animals mutant for ciliary opsin-1 had a smaller ciliary compartment and mutant larvae showed diminished pressure responses. The ciliary photoreceptors synaptically connect to the head multiciliary band that propels swimming via serotonergic motoneurons. Genetic inhibition of the serotonergic cells blocked pressure-dependent increases in ciliary beating. We conclude that ciliary photoreceptors function as pressure sensors and activate ciliary beating through serotonergic signalling during barokinesis.

show abstract

Whole-body connectome of a segmented annelid larva

Cited by 5 publications

References 71 publications

Mechanism of barotaxis in marine zooplankton

Mechanism of barotaxis in marine zooplankton

Hierarchical communities in the larval Drosophila connectome: Links to cellular annotations and network topology

Mechanism of barotaxis in marine zooplankton

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