Whole Blood Immunoassay Based on Centrifugal Bead Sedimentation

Schaff, Ulrich Y.; Sommer, Gregory J

doi:10.1373/clinchem.2011.162206

Cited by 50 publications

(39 citation statements)

References 26 publications

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“…Sedimentation-based sorting of functionalized beads; 109,110 sorting of biological cells 107 Sedimentation and filtration Centrifugation in combination with reservoir geometry; 65,69,71,104,115,119,120 integration of filtering structures 121,122 Spinning the CD induces rapid density-based separation and liquid flow through filters. reagents at specific temperatures and manual transfer of reagents before running the test.…”

Section: Resultsmentioning

confidence: 99%

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Lab-on-a-CD: A Fully Integrated Molecular Diagnostic System

et al. 2016

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The field of centrifugal microfluidics has experienced tremendous growth during the past 15 years, especially in applications such as lab-on-a-disc (LoD) diagnostics. The strength of LoD systems lies in its potential for development into fully integrated sample-to-answer analysis systems. This review highlights the technologies necessary to develop the next generation of these systems. In addition to outlining valving and other fluid-handling operations, we discuss the recent advances and future outlook in four categories of LoD processes: reagent storage, sample preparation, nucleic acid amplification, and analyte detection strategies.

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Section: Resultsmentioning

confidence: 99%

“…This process also separated the beads from the red blood cells in the case that they are present, and provided a washing step for the beads. 109 Koh et al performed a similar sedimentation step with only one bead type for the detection of botulinum toxin and achieved a lower limit of detection of 0.09 pg/mL. 110 …”

Section: Particle Sortingmentioning

confidence: 99%

Lab-on-a-CD: A Fully Integrated Molecular Diagnostic System

et al. 2016

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“…Towards this goal, we have developed multiplexed immunoassays that can be implemented in a portable microfluidic platform developed by our group previously. 29,30 The sensing method used was sandwich immunoassays performed using silica beads (Figure 1). High specificity antibodies (capture antibodies) were covalently attached to the silica beads using a PEG linker.…”

Section: Resultsmentioning

confidence: 99%

Centrifugal sedimentation immunoassays for multiplexed detection of enteric bacteria in ground water

et al. 2016

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Waterborne pathogens pose significant threat to the global population and early detection plays an important role both in making drinking water safe, as well as in diagnostics and treatment of water-borne diseases. We present an innovative centrifugal sedimentation immunoassay platform for detection of bacterial pathogens in water. Our approach is based on binding of pathogens to antibody-functionalized capture particles followed by sedimentation of the particles through a densitymedia in a microfluidic disk. Beads at the distal end of the disk are imaged to quantify the fluorescence and determine the bacterial concentration. Our platform is fast (20 min), can detect as few as $10 bacteria with minimal sample preparation, and can detect multiple pathogens simultaneously. The platform was used to detect a panel of enteric bacteria (Escherichia coli, Salmonella typhimurium, Shigella, Listeria, and Campylobacter) spiked in tap and ground water samples. V C 2016 AIP Publishing LLC. [http://dx

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“…An innovative modification in centrifugal microfluidics has been the implementation of bead-based immunoassays using density-media centrifugation (15). This allows bodily fluids, such as WB and saliva, to be analyzed directly because cells are separated by centrifugation from the analytes, which are captured on antibody-bearing beads.…”

Section: Centrifugation Using Density Mediamentioning

confidence: 99%

Microfluidic Sample Preparation for Medical Diagnostics

Cui

Rhee

Singh

et al. 2015

Annu. Rev. Biomed. Eng.

113

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Fast and reliable diagnoses are invaluable in clinical care. Samples (e.g., blood, urine, and saliva) are collected and analyzed for various biomarkers to quickly and sensitively assess disease progression, monitor response to treatment, and determine a patient's prognosis. Processing conventional samples entails many manual time-consuming steps. Consequently, clinical specimens must be processed by skilled technicians before antigens or nucleic acids are detected, and these are often present at dilute concentrations. Recently, several automated microchip technologies have been developed that potentially offer many advantages over traditional bench-top extraction methods. The smaller length scales and more refined transport mechanisms that characterize these microfluidic devices enable faster and more efficient biomarker enrichment and extraction. Additionally, they can be designed to perform multiple tests or experimental steps on one integrated, automated platform. This review explores the current research on microfluidic methods of sample preparation that are designed to aid diagnosis, and covers a broad spectrum of extraction techniques and designs for various types of samples and analytes.

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Whole Blood Immunoassay Based on Centrifugal Bead Sedimentation

Cited by 50 publications

References 26 publications

Lab-on-a-CD: A Fully Integrated Molecular Diagnostic System

Lab-on-a-CD: A Fully Integrated Molecular Diagnostic System

Centrifugal sedimentation immunoassays for multiplexed detection of enteric bacteria in ground water

Microfluidic Sample Preparation for Medical Diagnostics

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