Whole-bacterium SELEX of DNA aptamers for rapid detection of E.coli O157:H7 using a QCM sensor

Yu, Xiaofan; Chen, Fang; Wang, Ronghui; Li, Yanbin

doi:10.1016/j.jbiotec.2017.12.011

Cited by 130 publications

(70 citation statements)

References 54 publications

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“…The resulting linear equation is ip (µA) = −0.368n + 4.29, with a correlation coefficient of 0.996. In addition, the limit of detection (LOD) is 80 CFU/mL at a signal-to-noise ratio of 3, which is lower than other methods reported previously for the detection of E. coli (Table 1) [6,[42][43][44][45][46][47][48][49][50][51].…”

Section: Sensitivity and Reproducibility Of The Electrochemical Biosementioning

confidence: 70%

Development of an Electrochemical Biosensor for Rapid and Effective Detection of Pathogenic Escherichia coli in Licorice Extract

et al. 2019

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An aptamer-based electrochemical biosensor was successfully developed and applied in the rapid detection of pathogenic Escherichia coli (E. coli) in licorice extract. The thiolated capture probes were firstly immobilized on a gold electrode, and then the biotinylated aptamer probes for E. coli were introduced by hybridization with the capture probes. Due to the stronger interaction between the aptamer and the E. coli, a part of the biotinylated aptamers will dissociate from the capture probes in the presence of E. coli. The residual biotinylated aptamer probes can quantitatively bind with streptavidin-alkaline phosphatase. Subsequently, α-naphthyl phosphate substrate was catalytically hydrolyzed to generate electrochemical response, which could be recorded by a differential pulse voltammetry. The dependence of the peak current on the logarithm of E. coli concentration in the range from 5.0 × 10 2 colony forming units (CFU)/mL to 5.0 × 10 7 CFU/mL exhibited a linear trend with a detection limit of 80 CFU/mL. The relative standard deviation of 5 successive scans was 5.3%, 4.5% and 1.1% for 5.0 × 10 2 , 5.0 × 10 5 and 5.0 × 10 7 CFU/mL E. coli, respectively. In the detection of the licorice extract samples, the results obtained from the proposed strategy and traditional culture counting method were close to each other, but the time consumption was only~1/30 compared with the traditional method. These results demonstrate that the designed biosensor can be potentially utilized for rapid microbial examination in traditional Chinese medicine and relevant fields. Appl. Sci. 2019, 9, 295 2 of 15 bacterial colony morphology, color change, and biochemical reactions for quantitative detection. This detection method is economical and relatively simple to operate, but the detection time is generally 48-72 h or even longer, which cannot meet the needs for rapid detection. As an effective molecular biological detection technology, polymerase chain reaction (PCR) and the derived technology of PCR has been widely used in detecting E. coli [4,7]. PCR is based on the principle that DNA templates can denature at high temperatures, reactivate and extend (one cycle) at low temperatures by using appropriate DNA polymerase and primers. As the template DNA amplification product increases exponentially, increasing the number of cycles can magnify the specified DNA sequence by several million-fold in a short time, which greatly improves detection sensitivity [8]. However, PCR technology is costly and prone to produce false negatives [9], which limits the extensive application of PCR. In addition, enzyme-linked immunosorbent assay (ELISA), as a common immunological detection technology, has also been used in bacterial test [5,10]. ELISA technology undergoes a color change by the catalysis of the enzyme in the specific recognition process. Owing to the immune activity between antigen and antibody, ELISA method has superior specificity, high sensitivity and short detection time, and has been used in detection of different subtypes of E. col...

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Section: Sensitivity and Reproducibility Of The Electrochemical Biosementioning

confidence: 70%

Development of an Electrochemical Biosensor for Rapid and Effective Detection of Pathogenic Escherichia coli in Licorice Extract

et al. 2019

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“…Quartz crystal microbalance (QCM) is a mechanical biosensor that is widely used due to its capacity to track shifts in mass in sub-nanogram amounts. The change in mass using QCM biosensors is recognized by the resonant frequency of quartz crystal, and this technique is commonly used with extreme sensitivity for quantification of the whole cell of microorganisms [121]. Bayramoglu et al [122] designed A QCM-aptasensor to isolate and rapid detect Brucella melitensis in milk and milk products.…”

Section: Mechanical Biosensorsmentioning

confidence: 99%

Application of Biosensors for Detection of Pathogenic Food Bacteria: A Review

et al. 2020

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The use of biosensors is considered a novel approach for the rapid detection of foodborne pathogens in food products. Biosensors, which can convert biological, chemical, or biochemical signals into measurable electrical signals, are systems containing a biological detection material combined with a chemical or physical transducer. The objective of this review was to present the effectiveness of various forms of sensing technologies for the detection of foodborne pathogens in food products, as well as the criteria for industrial use of this technology. In this article, the principle components and requirements for an ideal biosensor, types, and their applications in the food industry are summarized. This review also focuses in detail on the application of the most widely used biosensor types in food safety.

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“…They have a multipurpose detection scale and sense various kinds of biomolecules from different specimens [54,55]. Piezoelectric nanosensors measure mass change and viscoelasticity by recording frequency and modifying a quartz crystal resonator [56,57]. The sensing needs isolation equipment that minimizes any hindrance effects because of the high sensitivity to environmental circumstances.…”

Section: Fundamental Of Nanosensorsmentioning

confidence: 99%