Application of Biosensors for Detection of Pathogenic Food Bacteria: A Review

Abstract

The use of biosensors is considered a novel approach for the rapid detection of foodborne pathogens in food products. Biosensors, which can convert biological, chemical, or biochemical signals into measurable electrical signals, are systems containing a biological detection material combined with a chemical or physical transducer. The objective of this review was to present the effectiveness of various forms of sensing technologies for the detection of foodborne pathogens in food products, as well as the criteria for industrial use of this technology. In this article, the principle components and requirements for an ideal biosensor, types, and their applications in the food industry are summarized. This review also focuses in detail on the application of the most widely used biosensor types in food safety.

Perioral Aerosol Sequestration Suction Device Effectively Reduces Biological Cross-Contamination in Dental Procedures

“…The limitation of our study is that bioluminescence, despite being able to identify living microorganisms, such as bacteria, dinoflagellates, and fungi, does not detect the presence of viruses. 13 Despite reports of measurable droplet and splatter reduction have encouraged the HVE use in routine dental practice while working with four hands, 29 other authors did not find neither significant droplet and splatter counts decrease nor environmental contamination reductions unless the HVE aspirator was subject to modifications. 30 In our study, the OBF device as provided by the manufacturer was attached to the standard HVE hose while permitting the OHP to operate with two hands without assistant.…”

“…12 Bioluminescence has not proven to date a direct correlation between viable microbial counts and detected ATP levels. 9,13 However, ATP-bioluminescence is a valuable tool for determining the efficiency of cleanliness procedures as well as to distinguish between live and dead cells, 13 but it is not efficient in detection environmental, not cell-bound ATP and viruses. Using ATP bioluminescence, Watanabe et al reported significant higher values of contamination and splatters in surfaces produced by droplet and splatter in operator masks, goggles, chest, and gowned right arm after dental procedures.…”

“…The limitation of our study is that bioluminescence, despite being able to identify living microorganisms, such as bacteria, dinoflagellates, and fungi, does not detect the presence of viruses. 13 …”

“…8 Measuring the concentration of adenosine triphosphate (ATP) by bioluminescence assay is a technique routinely used in hospitals and the food industry [9][10][11][12] for fast and accurate quantification of biological contamination on surfaces. ATP systems are able to detect dried organic materials and dead microorganisms 9 and to determine the efficacy of environmental cleanliness procedures even with low microbial counts, 13,14 but do not detect viruses. This measurement technique has been successfully used in a recent dentistry study which showed increased biocontamination levels on operator's goggles, masks, arms, and patient's goggles after standard dental procedures, demonstrating the ability of generated droplet and splatter to spread infection among operators and patients.…”

Applications of bioluminescence in biotechnology and beyond

“…211,212 This is the technique of choice when the speed and ease of analysis are of vital importance as alternative methods such as culturing or microorganisms usually take days to offer results, whilst techniques based on fluorescence need an external light source for excitation and do not discriminate between living and dead cells. 213 Bacterial bioluminescence is also used in the food industry to monitor the behaviour of Lux-tagged bacteria in situ in complex food systems, for problem-solving and for the development of modified and improved processing and storage purposes as discussed further below (Fig. 6).…”