White spot syndrome virus Manipulates Ubiquitin Gene Expression in Penaeus monodon

Vidya, R; Gireesh‐Babu, P.; Prasad, K. Pani

doi:10.1007/s13337-012-0113-0

Cited by 8 publications

(3 citation statements)

References 6 publications

(7 reference statements)

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“…This pathway also participated in viral infection and assists activities required of different aspect of virus life cycle, such as entry, replication and shedding [ 94 ]. Recently, various reports have been conducted showing the hijack of UPP pathway to regulate cellular intrinsic antiviral activities and innate immunity induced by WSSV [ 95 , 96 ]. E3 ubiquitin-protein ligase HUWE1 with the highest up changes after WSSV chanllenge was found in M .…”

Section: Discussionmentioning

confidence: 99%

A transcriptome study on Macrobrachium nipponense hepatopancreas experimentally challenged with white spot syndrome virus (WSSV)

Zhao

Sun

et al. 2018

PLoS ONE

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White spot syndrome virus (WSSV) is one of the most devastating pathogens of cultured shrimp, responsible for massive loss of its commercial products worldwide. The oriental river prawn Macrobrachium nipponense is an economically important species that is widely farmed in China and adult prawns can be infected by WSSV. However, the molecular mechanisms of the host pathogen interaction remain unknown. There is an urgent need to learn the host pathogen interaction between M. nipponense and WSSV which will be able to offer a solution in controlling the spread of WSSV. Next Generation Sequencing (NGS) was used in this study to determin the transcriptome differences by the comparison of control and WSSV-challenged moribund samples, control and WSSV-challenged survived samples of hepatopancreas in M. nipponense. A total of 64,049 predicted unigenes were obtained and classified into 63 functional groups. Approximately, 4,311 differential expression genes were identified with 3,308 genes were up-regulated when comparing the survived samples with the control. In the comparison of moribund samples with control, 1,960 differential expression genes were identified with 764 genes were up-regulated. In the contrast of two comparison libraries, 300 mutual DEGs with 95 up-regulated genes and 205 down-regulated genes. All the DEGs were performed GO and KEGG analysis, overall a total of 85 immune-related genes were obtained and these gene were groups into 13 functions and 4 KEGG pathways, such as protease inhibitors, heat shock proteins, oxidative stress, pathogen recognition immune receptors, PI3K/AKT/mTOR pathway, MAPK signaling pathway and Ubiquitin Proteasome Pathway. Ten genes that valuable in immune responses against WSSV were selected from those DEGs to furture discuss the response of host to WSSV. Results from this study contribute to a better understanding of the immune response of M. nipponense to WSSV, provide information for identifying novel genes in the absence of genome of M. nipponense. Furthermore, large number of transcripts obtained from this study could provide a strong basis for future genomic research on M. nipponense.

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Section: Discussionmentioning

confidence: 99%

A transcriptome study on Macrobrachium nipponense hepatopancreas experimentally challenged with white spot syndrome virus (WSSV)

Zhao

Sun

et al. 2018

PLoS ONE

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“…As for the Hsp40 gene, its responses to viral infection have been reported in halibut Paralichthys olivaceus [55]. In another study using the WSSV challenged tiger shrimp P. monodon, ubiquitin gene was down-regulated during the first 12 hours, but reversed in the following period [56]. Lastly, a study in red swamp crayfish, Procambarus clarkii, listed DnaJ (Hsp40), ubiquitin, and innexin (detailed below) proteins for their possible anti-WSSV roles [57].…”

Section: Nimav-1_lva Genes Involved In Host-pathogen Interactionmentioning

confidence: 93%

The Complete Genome of an Endogenous Nimavirus (Nimav-1_LVa) From the Pacific Whiteleg Shrimp Penaeus (Litopenaeus) Vannamei

Bao

Tang

Alcivar–Warren³

2020

Genes

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White spot syndrome virus (WSSV), the lone virus of the genus Whispovirus under the family Nimaviridae, is one of the most devastating viruses affecting the shrimp farming industry. Knowledge about this virus, in particular, its evolution history, has been limited, partly due to its large genome and the lack of other closely related free-living viruses for comparative studies. In this study, we reconstructed a full-length endogenous nimavirus consensus genome, Nimav-1_LVa (279,905 bp), in the genome sequence of Penaeus (Litopenaeus) vannamei breed Kehai No. 1 (ASM378908v1). This endogenous virus seemed to insert exclusively into the telomeric pentanucleotide microsatellite (TAACC/GGTTA)n. It encoded 117 putative genes, with some containing introns, such as g012 (inhibitor of apoptosis, IAP), g046 (crustacean hyperglycemic hormone, CHH), g155 (innexin), g158 (Bax inhibitor 1 like). More than a dozen Nimav-1_LVa genes are involved in the pathogen-host interactions. We hypothesized that g046, g155, g158, and g227 (semaphorin 1A like) were recruited host genes for their roles in immune regulation. Sequence analysis indicated that a total of 43 WSSV genes belonged to the ancestral/core nimavirus gene set, including four genes reported in this study: wsv112 (dUTPase), wsv206, wsv226, and wsv308 (nucleocapsid protein). The availability of the Nimav-1_LVa sequence would help understand the genetic diversity, epidemiology, evolution, and virulence of WSSV.

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“…Our results also showed that levels of ubiquitin transcripts varied depending on the WSSV post-infection time interval, as well as the viral load, both in cabs and shrimp. Vidya et al (2013) suggested that one of the viral strategies to keep progressive infection involves modifying host ubiquitination.…”

Section: Differential Expression In N Granulata and L Vannamei Relate...mentioning

confidence: 99%

Susceptibility of Neohelice granulata (Decapoda, Varunidae) to white spot syndrome virus (WSSV)

Moser

Marques

2022

Aquacult Int

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White Spot Syndrome Virus (WSSV) continues to be the most severe viral pathogen to the shrimp industry worldwide. Paci c white shrimp Litopenaeus vannamei is particularly affected by WSSV and this virus has been detected in a wide range of wild crustaceans, including penaeid and non-penaeid shrimp, cray sh, as well as crabs. Crabs have been considered as a dangerous threat to shrimp farms because they are generally believed to be highly tolerant to WSSV and to remain infected for long periods without signs of disease. The burrowing crab Neohelice (= Chasmagnathus) granulata can be commonly found in the surroundings of the shrimp farms in southern Brazil. Here, we investigated the susceptibility of N. granulata to WSSV infection in comparison to L. vannamei. WSSV infectability and host susceptibility were investigated by viral challenge (intramuscular injection) in both species. Viral load in challenged animals was quanti ed by qPCR in both hemolymph and gills. Furthermore, the transcript levels of sixteen target genes related to the molecular defense status were assessed. The results revealed that N. granulate experimentally infected by WSSV, as well as those naturally infected, showed lethargy, lack of appetite and later gross signs of the disease. Moreover, N. granulate showed to be less susceptible to WSSV when compared to L. vannamei. While no death was observed in crabs before a post-challenge interval of 120 h; shrimp death was seen as early as 48 hours post-infection. Comparative viral load was also assessed by qPCR in gills of captured wild crabs and farmed shrimp naturally infected by WSSV. Transcript levels of target genes were also investigated after WS-challenge between 24 and 48 hpi in shrimp and between 96 and 120 hpi in crab. Differences in gene transcripts were particularly noteworthy with the increase of viral copies at 10 6 copies.µl − 1 . These results indicated that WSSV infection modulated genes related of important cellular responses, such as apoptosis, chaperone function and ion storage, in both shrimp and crab.

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White spot syndrome virus Manipulates Ubiquitin Gene Expression in Penaeus monodon

Cited by 8 publications

References 6 publications

A transcriptome study on Macrobrachium nipponense hepatopancreas experimentally challenged with white spot syndrome virus (WSSV)

A transcriptome study on Macrobrachium nipponense hepatopancreas experimentally challenged with white spot syndrome virus (WSSV)

The Complete Genome of an Endogenous Nimavirus (Nimav-1_LVa) From the Pacific Whiteleg Shrimp Penaeus (Litopenaeus) Vannamei

Susceptibility of Neohelice granulata (Decapoda, Varunidae) to white spot syndrome virus (WSSV)

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