Landfill leachate is a complex environmental problem. Here, we used immobilized Ganoderma australe and crude enzyme to treat landfill leachate. The treatment by immobilized G. australe achieved 50% and 32% removals for leachate BOD5 and COD, respectively. Totals of 58%, 57%, and 62% of BOD, COD, and NH3-N percentage removal were obtained when the leachate previously treated with immobilized G. australe was continuously treated with crude enzymes. Using a continuous treatment with crude enzymes achieves higher percentages of COD and NH3-N removal.