White-Light-Exciting, Layer-by-Layer-Assembled ZnCdHgSe Quantum Dots/Polymerized Ionic Liquid Hybrid Film for Highly Sensitive Photoelectrochemical Immunosensing of Neuron Specific Enolase

Abstract

ZnCdHgSe quantum dots (QDs) functionalized with N-acetyl-l-cysteine were synthesized and characterized. Through layer-by-layer assembling, the ZnCdHgSe QDs was integrated with a polymerized 1-decyl-3-[3-pyrrole-1-yl-propyl]imidazolium tetrafluoroborate (PDPIT) ionic liquid film modified indium tin oxide (ITO) electrode to fabricated a photoelectrochemical interface for the immobilization of rabbit antihuman neuron specific enolase (anti-NSE). After being treated with glutaraldehyde vapor and bovine serum albumin successively, an anti-NSE/ZnCdHgSe QDs/PDPIT/ITO sensing platform was established. Simplely using a white-light LED as an excitation source, the immunoassay of neuron specific enolase (NSE) was achieved through monitoring the photocurrent variation. The polymerized ionic liquid film was demonstrated to be an important element to enhance the photocurrent response of ZnCdHgSe QDs. The anti-NSE/ZnCdHgSe QDs/PDPIT/ITO based immunosensor presents excellent performances in neuron specific enolase determination. The photocurrent variation before and after being interacted with NSE exhibits a good linear relationship with the logarithm of its concentration (log cNSE) in the range from 1.0 pg mL(-1) to 100 ng mL(-1). The limit of detection of this immunosensor is able to reach 0.2 pg mL(-1) (S/N = 3). The determination of NSE in clinical human sera was also demonstrated using anti-NSE/ZnCdHgSe QDs/PDPIT/ITO electrode. The results were found comparable with those obtained by using enzyme-linked immunosorbent assay method.

Nanoplatelet cryoaerogels with potential application in photoelectrochemical sensing

“…[1][2][3] One particular advantage of this sensor type is the independence of the excitation signal (light) and the detection signal (current), which does not only yield low detection limits but could also enable the detection of multiple analytes by one and the same probe. 1,4 To date, numerous types of semiconductor NP sensitised photoelectrodes for sensing applications have already been developed, including self-assembled NP monolayers, [5][6][7][8][9] and layer-by-layer, [10][11][12][13][14] and cross-linked 15,16 assemblies. Multilayered systems in general were shown to exhibit distinctly higher photocurrents than comparable monolayer systems, with the photocurrent being observed to scale with the number of layers deposited.…”

Colorimetric detection of Al(iii) in vermicelli samples based on ionic liquid group coated gold nanoparticles

“…We design copper ions sensors by click chemistry 18 and organic molecular ligands-coated Au NPs. 44,45 Ionic liquids are helpful for the enhancement of the sensitivity. 20 Making use of meso-2,3-dimercaptosuccinic acid (DMSA)functionalized Au NPs, chromium ions can be determined.…”

“…43 Ionic liquids can be modied on the surface of nanoparticles, such as Au NPs, quantum dots, to carry out protein assays with the changes of electrochemical signals. 44,45 Ionic liquids are helpful for the enhancement of the sensitivity. Besides electrochemical sensors, other kinds of ionic liquid sensors are also reported.…”

Paper-Based Surface-Enhanced Raman Scattering Lateral Flow Strip for Detection of Neuron-Specific Enolase in Blood Plasma

“…Therefore, great efforts have been devoted to developing portable biosensors toward POC testing. Recently, several biosensors have been employed for NSE biomarker detection, including electrochemistry, − chemiluminescence, and field-effect transistor . However, most of these biosensors were tested in buffer solutions and did not demonstrate the direct detection of NSE biomarker in human fluidic samples.…”