Which morphological forms of the fungus<i>Aureobasidium pullulans</i>are responsible for pullulan production?

Campbell, Bradley S.; Siddique, Abu-Baker M.; McDougall, Barbara M.; Seviour, Robert J.

doi:10.1016/s0378-1097(04)00076-x

Cited by 53 publications

(31 citation statements)

References 13 publications

(19 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…With NH 4 + as N source, EPS yields were markedly affected in an airlift reactor by the aeration rate applied (Roukas and Mantzouidou, 2001). They were optimal at 2 vvm, where the population was predominantly mycelia, which might question the widely accepted causal relationship between pullulan synthesis and unicellular forms of this fungus (Campbell et al, 2004;Simon et al, 1993). However, as with many similar studies, the EPS quantified in this work was assumed to be solely pullulan, yet no experimental evidence was presented to support the claim.…”

Section: The α-Glucan Pullulanmentioning

confidence: 70%

“…It seems clear (Gibbs and Seviour, 1996a) that NO 3 − favours production of unicells while mycelial growth occurs predominantly with NH 4 + . Enough direct and indirect evidence exists to support the view that pullulan production is restricted to unicellular swollen cells and chlamydospores (Campbell et al, 2004;Simon et al, 1993), while mycelial forms of this polymorphic fungus probably synthesize other EPS material (e.g. Orr et al, 2009).…”

Section: The α-Glucan Pullulanmentioning

confidence: 98%

See 1 more Smart Citation

Operating bioreactors for microbial exopolysaccharide production

Seviour¹,

McNeil²,

Fazenda³

et al. 2010

Critical Reviews in Biotechnology

View full text Add to dashboard Cite

There is considerable interest in exploiting the novel physical and biological properties of microbial exopolysaccharides in industry and medicine. For economic and scientific reasons, large scale production under carefully monitored and controlled conditions is required. Producing exopolysaccharides in industrial fermenters poses several complex bioengineering and microbiological challenges relating primarily to the very high viscosities of such culture media, which are often exacerbated by the producing organism's morphology. What these problems are, and the strategies for dealing with them are discussed critically in this review, using pullulan, curdlan, xanthan, and fungal β-glucans as examples of industrially produced microbial exopolysaccharides. The role of fermenter configuration in their production is also examined.

show abstract

Section: The α-Glucan Pullulanmentioning

confidence: 70%

Section: The α-Glucan Pullulanmentioning

confidence: 98%

Operating bioreactors for microbial exopolysaccharide production

Seviour¹,

McNeil²,

Fazenda³

et al. 2010

Critical Reviews in Biotechnology

View full text Add to dashboard Cite

show abstract

“…Yeast cells are mainly responsible for pullulan production (1). Till date most of the studies have been reported on mesophilic strain of this fungus which produces pullulan up to level of 30-36 g/l in flask shake experiments or in fermentor experiments at a temperature range of 24-32 o C at different physico-chemical conditions (4,7,8,9,11,13,15,17).…”

Section: Introductionmentioning

confidence: 99%

Production of Pullulan from a high yielding strain of Aureobasidium pullulans in non-stirred flask-type fermentation system

Singh¹,

Gaur

Jamal

et al. 2017

J Microbiol Biotech Res

View full text Add to dashboard Cite

Aureobasidium pullulans, popularly known as black yeast, is one of the most widespread saprophytic fungus associated with wide range of terrestrial and aquatic habitats. The fungus has widely been employed in production of an economically important polysaccharide pullulan. Total 50 isolates of Aureobasidium pullulans were isolated from different flowers and leaves samples, out of which 10 thermotolerant strains produced pullulan. One thermotolerant non-melanin pullulan producing strain, designated as produced highest pullulan (6.0±0.003 g/100ml)

show abstract

“…Different strains of A. pullulans produce substantially different titers and yields (on sugar substrates) of pullulan (Campbell et al, 2004;Gibson and Coughlin, 2002;West and Strohfus, 2001). Compared to the other strain, the NRRL Y-2311-1 produced a higher yield of unpigmented and reasonably higher molecular weight pullulan when cultivated under the conditions reported herein.…”

Section: Discussionmentioning

confidence: 99%

Enhanced production of pullulan by two strains of A. pullulans with different concentrations of soybean oil in sucrose solution in batch fermentations

Sena

Costelli

Gibson

et al. 2006

Braz. J. Chem. Eng.

View full text Add to dashboard Cite

-Aureobasidium pullulans is a microorganism that produces pullulan (homopolysaccharide) extracellularly through a fermentation process with sugars (maltose, d-xylose, sucrose and starch) as its carbon source. Pullulan is a linear polysaccharide of D-glycopyranose containing (1 → 4)-α and (1 → 6)-α linkages at a 2:1 ratio, is highly soluble in water and has various applications in the food, packaging, film and pharmaceutical industries. Lipids, primarily oils, having antifoaming properties as well as nutritional particularities, are considered an essential additional carbon source for the growth of microorganisms, especially fungi. These nutrient sources are very important for the maintenance of microorganism cells. In fact, these positive effects are only achieved when the right source is added at both the right time and the right dosage into the broth of the fermentation process. In this research on pullulan production with the strains NRRL Y-6220 and NRRL Y-2311-1, it was found that the latter strain achieved better results for undesirable pigment formation, pullulan titer, time of maximum production (96 hours) and biomass yields than strain NRRL Y-6220, which also showed suitable results for biomass yields and cell morphology. However, the dark pigmentation of the strain NRRL Y-6220, formed through the process, makes its application unacceptable for foods and pharmaceuticals. Strain NRRL Y-2311-1 was shown to be a promising potential industrial microorganism, whose applications should be studied more in depth.

show abstract

Which morphological forms of the fungusAureobasidium pullulansare responsible for pullulan production?

Cited by 53 publications

References 13 publications

Operating bioreactors for microbial exopolysaccharide production

Operating bioreactors for microbial exopolysaccharide production

Production of Pullulan from a high yielding strain of Aureobasidium pullulans in non-stirred flask-type fermentation system

Enhanced production of pullulan by two strains of A. pullulans with different concentrations of soybean oil in sucrose solution in batch fermentations

Contact Info

Product

Resources

About