Production of Pullulan from a high yielding strain of Aureobasidium pullulans in non-stirred flask-type fermentation system

Abstract

Aureobasidium pullulans, popularly known as black yeast, is one of the most widespread saprophytic fungus associated with wide range of terrestrial and aquatic habitats. The fungus has widely been employed in production of an economically important polysaccharide pullulan. Total 50 isolates of Aureobasidium pullulans were isolated from different flowers and leaves samples, out of which 10 thermotolerant strains produced pullulan. One thermotolerant non-melanin pullulan producing strain, designated as produced highest pullulan (6.0±0.003 g/100ml)

A Novel Media Optimized for Production of Pullulan in Flask Type Fermentation System

“…Similar trend in pullulan production was observed by in other works of same worker (Singh et al, 2012;Singh et al, 2016;Singh et al, 2017). Similarly maximum biomas production was also observed at 96h, since formation of biomass directly depends on pullulan formation.…”

“…Different workers have reported pullulan production at different pH range (Singh et al, 2012;Singh et al, 2016;Singh et al, 2017;Thirumavalan et al, 2008;Thirumavalan et al, 2009).…”

“…Carbon sources play a vital role in the production of pullulan (Singh et al, 2012;Singh et al, 2016;Singh et al, 2017) (Fig. 4).…”

“…The sugars such as sucrose, glucose, fructose, maltose, starch support pullulan production by A. pullulans (Singh, Gaur, Tiwari and Gaur, 2012;Singh, Gaur, Bansal, Biswas, Pandey et al, 2016;Singh, Gaur, Bansal, Jamal, Pandey et al, 2017). A number of complex carbon sources have been reported for pullulan production, including peat hydrolysate, cornmeal hydrolysates, corn syrup, fuel ethanol fermentation stillage, carob pod, grape skin pulp, olive oil and sucrose, beet molasses, hydrolyzed potato starch, spent grain liquor, jaggery, cashew fruit juice, coconut water and milk, Jerusalem artichoke and on mixture of potato starch hydrolysate and sucrose (Boa and LeDuy, 1984;Boa and LeDuy, 1987;Leather and Gupta, 1994;Roukas and Biliaderis, 1995;Isralidies, Smith, Harthill, Barnett, Bambalov and Scanlon, 1998;Youseef, Biliaders and Roukas, 1998;Roukas, 1998;Roukas and Liakopoulou, 1999;Barnett, Smith, Scanlon, Israilides, 1999;Roukas, 1999;Vijayendra, Bansal, Prasad and Nand, 2001;Thirumavalan, Manikkandan and Dhaneseskar, 2008;Thirumavalan, Manikkandan and Dhaneseskar, 2009;Xia, Xu, Liu, Xu, Wang and Li, 2017;Chao, Ma, Chang and Xue, 2017).…”

Valorization of cassava waste for pullulan production by Aureobasidium pullulans MTCC 1991

“…Magnesium plays a vital role in cell growth and influences the metabolic pathway for the production of exopolysaccharide. 44 The interaction effect of MgSO 4 ⋅7H 2 O with yeast extract, K 2 HPO 4 , (NH 4 ) 2 SO 4 , and NaCl on pullulan yield was illustrated in Figure 4(c), (f), (h), and (j), respectively. The results showed that the maximum pullulan production was obtained at 0.3 g/L than 0.1 g/L MgSO 4 ⋅7H 2 O concentration (Figure 3).…”