Whey protein enhances normal inflammatory responses during cutaneous wound healing in diabetic rats

Ebaid, Hossam; Salem, Amir M.H.; Sayed, Abdalla A.; Metwalli, Ali

doi:10.1186/1476-511x-10-235

Cited by 92 publications

(82 citation statements)

References 44 publications

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“…54 Neutrophils have been shown to act as an early response immune cells that play a key role in the healing of an epithelium-debridement wound. 31 A large number of NK cells infiltrate into the cornea and participate in the modulation of Figure 9 Schematic diagram deciphering the role of balanced IL-1beIL-1 receptor antagonist (IL-1Ra) signaling in mediating epithelial wound healing and its defects in the diabetic corneas.…”

Section: Il-1ra and Diabetic Cornea Wound Healingmentioning

confidence: 99%

Targeting Imbalance between IL-1β and IL-1 Receptor Antagonist Ameliorates Delayed Epithelium Wound Healing in Diabetic Mouse Corneas

Yan

Gao

Sun

et al. 2016

The American Journal of Pathology

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fanxq@sh163. net.Patients with diabetes mellitus often develop corneal complications and delayed wound healing. How diabetes might alter acute inflammatory responses to tissue injury, leading to delayed wound healing, remains mostly elusive. Using a streptozotocin-induced type I diabetes mellitus mice and corneal epithelium-debridement wound model, we discovered that although wounding induced marked expression of IL-1b and the secreted form of IL-1 receptor antagonist (sIL-1Ra), diabetes suppressed the expressions of sIL-1Ra but not IL-1b in healing epithelia and both in whole cornea. In normoglycemic mice, IL-1b or sIL-1Ra blockade delayed wound healing and influenced each other's expression. In diabetic mice, in addition to delayed reepithelization, diabetes weakened phosphatidylinositol 3-kinaseeAkt signaling, caused cell apoptosis, diminished cell proliferation, suppressed neutrophil and natural killer cell infiltrations, and impaired sensory nerve reinnervation in healing mouse corneas. Local administration of recombinant IL-1Ra partially, but significantly, reversed these pathological changes in the diabetic corneas. CXCL10 was a downstream chemokine of IL-1beIL-1Ra, and exogenous CXCL10 alleviated delayed wound healing in the diabetic, but attenuated it in the normal corneas. In conclusion, the suppressed early innate/inflammatory responses instigated by the imbalance between IL-1b and IL-1Ra is an underlying cause for delayed wound healing in the diabetic corneas. Local application of IL-1Ra accelerates reepithelialization and may be used to treat chronic corneal and potential skin wounds of diabetic patients. (Am J Pathol 2016 http://dx

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Section: Il-1ra and Diabetic Cornea Wound Healingmentioning

confidence: 99%

Targeting Imbalance between IL-1β and IL-1 Receptor Antagonist Ameliorates Delayed Epithelium Wound Healing in Diabetic Mouse Corneas

Yan

Gao

Sun

et al. 2016

The American Journal of Pathology

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“…In addition, TGF-β directly stimulates collagen synthesis and decreases extracellular matrix degradation by fibroblasts (Blakytny and Jude 2006). Collagen is critical for the strength and integrity of extracellular matrix and for epithelialization during the later stages of wound healing (Brancato and Albina 2011;Ebaid et al 2011). In our study, WHR was significantly higher in rats from the ES group than in those from the DC group, and the accelerated wound healing observed in the ES group might be due to increased growth factor synthesis.…”

Section: Discussionmentioning

confidence: 99%

High-Voltage Pulsed Current Stimulation Enhances Wound Healing in Diabetic Rats by Restoring the Expression of Collagen, <i>α</i>-Smooth Muscle Actin, and TGF-<i>β</i>1

Kim

Cho

Lee

2014

Tohoku J. Exp. Med.

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Impaired wound healing is a common complication of diabetes mellitus and a major morbidity that leads to pain and severely diminished quality of life. Diabetic wounds are commonly associated with defective immune cell responses or abnormality of extracellular matrix. Various types of electrical stimulation interventions have been used to promote tissue healing. However, it is unclear whether high-voltage pulsed current stimulation (HVPCS) enhances diabetic wound healing. In this study, the effects of HVPCS on wound healing were investigated in diabetic rats. Three groups of rats (10 per group) were used: non-diabetic control, diabetic control, and diabetic rats that were administered HVPCS for 40 minutes daily for 1 week. Rats from control groups were administered sham interventions. Dorsal incision wounds were generated in all animals, and wound-healing rate was determined during one-week intervention. After interventions, we measured the relative expression levels of collagen type I (collagen-I), α-smooth muscle actin (α-SMA), and transforming growth factor-β1 (TGF-β1) mRNAs in the wounded skin. Wound closure was delayed in diabetic control rats compared to the non-diabetic control rats, and the diabetic control rats showed the reduced expression levels of collagen-I, α-SMA and TGF-β1 mRNAs. Importantly, compared to diabetic control rats, rats with HVPCS showed accelerated wound closure and healing (p < 0.01) and restored expression levels of collagen-I (p = 0.02), α-SMA (p = 0.04), and TGF-β1 (p = 0.01) mRNAs. In conclusion, HVPCS may be beneficial for enhancing the healing of diabetic wounds by restoring the expression levels of TGF-β1, collagen-I, and α-SMA.

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“…The dose and supplementation period was established in accordance with the literature [9][10][11] . The animals in the one control and one normal control group groups underwent the same procedure, but they only received water.…”

Section: E T H O D Smentioning

confidence: 99%

“…The results of all the physical-chemical analyses, sensory tests and physiological parameters were expressed as mean ± standard error of the mean and submitted to Anova followed by Tukey's test (p<0.05) due to normal distribution and because these tests are used in this type of study 4,[9][10][11]28 . We used the Statistical Analysis System (SAS, Institute Inc., Cary, North Carolina, United States, 2006) statistical software version 9.1 and GraphPad Prism 5.0 program (Prism Software ® , Inc., La Jolla, California, United States).…”

Section: E T H O D Smentioning

confidence: 99%

Functional properties and sensory testing of whey protein concentrate sweetened with rebaudioside A

et al. 2016

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Objective: To develop a natural dietary product with functional benefits for diabetic patients. Whey protein concentrate was obtained through the separation membrane processes and sweetened with rebaudioside A. This product was submitted to sensory testing in humans and used to evaluate possible functional properties in male Wistar rats models with diabetesMellitus induced by streptozotocin. Methods: Two concentrates were produced. Only the second showed protein content of 74.3 and 17.3% of lactose was used as supplementation in induced diabetic rats. This concentrate was obtained from the concentration by reverse osmosis system (180 k Daltons), followed by nanofiltration in a 500 k Daltons membrane and spray drying at 5.0% solution of the first concentrate developed. The concentrate was sweetened with rebaudioside A (rebaudioside A 26 mg/100 g concentrate). All procedures were performed at the Center for Studies in Natural Products, at the Universidade Estadual de Maringá. Three experimental groups were established (n=6): two groups of diabetic animals, one control group and one supplemented group; and a control group of normal mice (non-diabetic). The supplemented group received concentrates sweetened with rebaudioside A in a dose of 100 mg/kg bw/day by an esophageal tube for 35 days. Fasting, the fed state and body weight were assessed weekly for all groups. At the end of the supplementation period, the following were analyzed: plasma parameters of glucose, total cholesterol, triglycerides and fructosamine; the serum levels of aspartate aminotransferase and alanine aminotransferase, water and food intake. Organs and tissues were removed and weighed to assess mass and anatomical changes. Results: The product presented 74% of proteins and 17% of lactose and showed satisfactory sensory testing by the addition of 26 mg of rebaudioside A/100 g concentrate. Supplementation of the product reduced hyperglycemia, plasma fructosamine levels, triglycerides and total cholesterol, and improved body weight gain of streptozotocin-induced diabetic rats. Conclusion: Whey protein concentrate with substantial content of protein (above 70%) and low lactose was obtained through the membrane separation processes. The addition of rebaudioside A at the concentration of 26 mg/100 g rebaudioside A proved to be as sweet as sucralose with satisfactory sensory testing, which indicates that this is a non-caloric natural sweetener that can replace artificial sweeteners. The product (whey protein concentrate sweetened with rebaudioside A) presented important functional properties and reduced the metabolic disorders caused by the syndrome.

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Whey protein enhances normal inflammatory responses during cutaneous wound healing in diabetic rats

Cited by 92 publications

References 44 publications

Targeting Imbalance between IL-1β and IL-1 Receptor Antagonist Ameliorates Delayed Epithelium Wound Healing in Diabetic Mouse Corneas

Targeting Imbalance between IL-1β and IL-1 Receptor Antagonist Ameliorates Delayed Epithelium Wound Healing in Diabetic Mouse Corneas

High-Voltage Pulsed Current Stimulation Enhances Wound Healing in Diabetic Rats by Restoring the Expression of Collagen, <i>α</i>-Smooth Muscle Actin, and TGF-<i>β</i>1

Functional properties and sensory testing of whey protein concentrate sweetened with rebaudioside A

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