When AIE meets enzymes

Feng, Guangfu; Liao, Sijie; Liu, Yufeng; Zhang, Huaizu; Luo, Xingyu; Zhou, Xiangming; Fang, Jun

doi:10.1039/d2an00679k

Cited by 9 publications

(4 citation statements)

References 98 publications

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“…Moreover, hydrophilic peptides have been utilized as enzyme-responsive units and water-solubility promoters for the "turn-on" detection of other enzymes, including proteases, casein kinase II, furin, and autophagy-specific enzymes [147][148][149][150][151][152][153][154]. Several excellent review articles have extensively covered this topic, focusing on the AIE effect [155][156][157][158]. The AIE principle can be effectively combined with other photophysical quenching mechanisms, such as excited-state intramolecular proton transfer (ESIPT) and photoinduced electron transfer [159][160][161][162][163].…”

Section: Aie-based Assaysmentioning

confidence: 99%

“…Moreover, hydrophilic peptides have been utilized as enzyme-responsive units and water-solubility promoters for the “turn-on” detection of other enzymes, including proteases, casein kinase II, furin, and autophagy-specific enzymes [ 147 , 148 , 149 , 150 , 151 , 152 , 153 , 154 ]. Several excellent review articles have extensively covered this topic, focusing on the AIE effect [ 155 , 156 , 157 , 158 ].…”

Section: Fluorescent Assaysmentioning

confidence: 99%

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Advancements in Biosensors Based on the Assembles of Small Organic Molecules and Peptides

Deng,

Chang,

Liu

et al. 2023

Biosensors

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Over the past few decades, molecular self-assembly has witnessed tremendous progress in a variety of biosensing and biomedical applications. In particular, self-assembled nanostructures of small organic molecules and peptides with intriguing characteristics (e.g., structure tailoring, facile processability, and excellent biocompatibility) have shown outstanding potential in the development of various biosensors. In this review, we introduced the unique properties of self-assembled nanostructures with small organic molecules and peptides for biosensing applications. We first discussed the applications of such nanostructures in electrochemical biosensors as electrode supports for enzymes and cells and as signal labels with a large number of electroactive units for signal amplification. Secondly, the utilization of fluorescent nanomaterials by self-assembled dyes or peptides was introduced. Thereinto, typical examples based on target-responsive aggregation-induced emission and decomposition-induced fluorescent enhancement were discussed. Finally, the applications of self-assembled nanomaterials in the colorimetric assays were summarized. We also briefly addressed the challenges and future prospects of biosensors based on self-assembled nanostructures.

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Section: Aie-based Assaysmentioning

confidence: 99%

Section: Fluorescent Assaysmentioning

confidence: 99%

Advancements in Biosensors Based on the Assembles of Small Organic Molecules and Peptides

Deng,

Chang,

Liu

et al. 2023

Biosensors

View full text Add to dashboard Cite

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“…Such materials have been used in a broad range of fields, including organic optoelectronic devices, biosensing, bioimaging, photodynamic therapy and photothermal therapy [ 34 , 35 , 36 , 37 ]. By conjugating fluorogens with the target ligands, many AIE chemo/biosensors have been designed for the determination of various analytes, such as ions, small molecules, microenvironment sensing, biological macromolecules, cellular processes and pathogens [ 38 , 39 , 40 ]. The aggregation of AIEgens can be triggered by different stimuli, including solubility change, hydrophobic assembly, hydrogen bonding, target–receptor binding and electrostatic interactions.…”

Section: Introductionmentioning

confidence: 99%

Background-Quenched Aggregation-Induced Emission through Electrostatic Interactions for the Detection of Poly(ADP-ribose) Polymerase-1 Activity

et al. 2023

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Poly(ADP-ribose) polymerase-1 (PARP1) is a potential biomarker and therapeutic target for cancers that can catalyze the poly-ADP-ribosylation of nicotinamide adenine dinucleotide (NAD+) onto the acceptor proteins to form long poly(ADP-ribose) (PAR) polymers. Through integration with aggregation-induced emission (AIE), a background-quenched strategy for the detection of PARP1 activity was designed. In the absence of PARP1, the background signal caused by the electrostatic interactions between quencher-labeled PARP1-specitic DNA and tetraphenylethene-substituted pyridinium salt (TPE-Py, a positively charged AIE fluorogen) was low due to the fluorescence resonance energy transfer effect. After poly-ADP-ribosylation, the TPE-Py fluorogens were recruited by the negatively charged PAR polymers to form larger aggregates through electrostatic interactions, thus enhancing the emission. The detection limit of this method for PARP1 detection was found to be 0.006 U with a linear range of 0.01~2 U. The strategy was used to evaluate the inhibition efficiency of inhibitors and the activity of PARP1 in breast cancer cells with satisfactory results, thus showing great potential for clinical diagnostic and therapeutic monitoring.

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“…Abnormal manifestations of enzyme activities could be indicative of humans’ health status. Thus, enzymes can be used as biomarkers for disease diagnosis [ 2 , 3 , 4 ]. For example, changes in the activity and content of proteases may cause some diseases, such as cancers, acquired immune deficiency syndrome, and neurodegenerative diseases [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Biosensors for the Detection of Enzymes Based on Aggregation-Induced Emission

et al. 2022

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Enzymes play a critical role in most complex biochemical processes. Some of them can be regarded as biomarkers for disease diagnosis. Taking advantage of aggregation-induced emission (AIE)-based biosensors, a series of fluorogens with AIE characteristics (AIEgens) have been designed and synthesized for the detection and imaging of enzymes. In this work, we summarized the advances in AIEgens-based probes and sensing platforms for the fluorescent detection of enzymes, including proteases, phosphatases, glycosidases, cholinesterases, telomerase and others. The AIEgens involve organic dyes and metal nanoclusters. This work provides valuable references for the design of novel AIE-based sensing platforms.

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When AIE meets enzymes

Abstract: With the rapid development of physiopathology and the surge in demand for comprehension of micro-scale physiological events, AIE-based bio-probes are found superior to presenting precise and practical results in enzyme...

Cited by 9 publications

References 98 publications

Advancements in Biosensors Based on the Assembles of Small Organic Molecules and Peptides

Advancements in Biosensors Based on the Assembles of Small Organic Molecules and Peptides

Background-Quenched Aggregation-Induced Emission through Electrostatic Interactions for the Detection of Poly(ADP-ribose) Polymerase-1 Activity

Biosensors for the Detection of Enzymes Based on Aggregation-Induced Emission

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