Wheat (Triticum aestivum): In Vitro Production and Utilization of Doubled Haploids

Picard, E.; Rode, Andrei; Doussinault, Gérard; Rousset, M.; Rives, M.

doi:10.1007/978-3-642-61499-6_5

Cited by 16 publications

(8 citation statements)

References 40 publications

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“…Walsh et al (1973) observed that there were differences in vigour of DH 1 and DH 2 seedlings, and recommended that DH lines should be advanced one generation from DH 1 before their performance relative to conventional check cultivars is evaluated. In this study, the yield performance and 1000 kernel weight of the third generation of DH lines (DH 3 seeds) were investigated using a randomized complete block design.…”

Section: Morphological Variations Among Microsporederived H 0 Plantsmentioning

confidence: 99%

“…Therefore, DH lines have the potential for use in plant breeding, mutagenesis, in vitro selection, molecular mapping and plant transformation . To confirm the advantages of DH lines in plant breeding, genetic stability of DH progenies has been studied widely in the past 20 yr as reviewed in wheat De Buyser and Henry 1986;Hu and Yang 1986;Picard 1990;Maheshwari et al 1995), barley (Choo et al 1985Pickering and Devaux 1992), rice (Chen 1986;Alejar et al 1995) and corn (Wu 1986;Genovesi 1990). Results indicate that about 90% of the doubled lines were genetically uniform.…”

mentioning

confidence: 99%

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Performance of isolated microspore-derived doubled haploids of wheat (Triticum aestivum L.)

Hu¹,

Kasha²

1997

Can. J. Plant Sci.

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. 1997. Performance of isolated microspore-derived doubled haploids of wheat (Triticum aestivum L.). Can. J. Plant Sci. 77: 549-554. Ploidy level, genetic stability and field performance of isolated microspore-derived wheat plants were evaluated. The ploidy levels of isolated microspore-derived wheat plants from cv. Chris and reciprocal crosses of Chris × Sinton were determined by two methods, namely chromosome counts of root tip cells and flow cytometric measurement of nuclear DNA content from leaves. Both methods gave similar results with the frequencies of spontaneous chromosome doubling and completely fertile plants among microspore-derived H 0 plants of wheat being about 80% and 75%, respectively, based on two H 0 populations. Only 1.7% were aneuploid and 16% were haploid. Spontaneous mutation frequencies were low with 1 of 124 Chris DH plants having a recessive mutant for lemma awns while three were grass-like plants in the H 0 generation. The field-measured traits of microspore-derived DH lines were similar to the check by the third generation. Ninety-two percent and 70% of Chris DH lines had 1000-kernel weights and yields similar to the check, respectively. These results indicate that microspore-derived DH lines should have good potential for applications in plant breeding, in vitro selection, plant transformation and genetic studies.

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Section: Morphological Variations Among Microsporederived H 0 Plantsmentioning

confidence: 99%

mentioning

confidence: 99%

Performance of isolated microspore-derived doubled haploids of wheat (Triticum aestivum L.)

Hu¹,

Kasha²

1997

Can. J. Plant Sci.

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“…Androgenetic capacity is defined as the ability of the male gametic cell to reverse its development, giving rise to haploid green plantlets that become completely homozygous and fully fertile after spontaneous or colchicine induced genome duplication (PICARD et al, 1990;PETERS et al, 1999).…”

Section: A Obtenção Na Cevada De Plantas Haplóides In Vitro E a Posmentioning

confidence: 99%

Genotypic differences in proembryoid development and green plantlets regeneration through androgenesis in barley varieties

et al. 2008

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“…Desta forma, a utilização de câmaras de cultivo e/ou casas de vegetação com alto rigor no controle de temperatura tem se mostrado indispensável na produção de plantas haploides androgenéticos de trigo. Os trabalhos realizados até o momento indicam que as variações entre baixas e altas temperaturas afetam mais significativamente a resposta androgenética do que sua ação em faixas isoladas (somente alta ou baixa temperatura) (HEBERLE-BORS; REINERT, 1979, HENRY;BUYSER, 1990;HUANG, 1990;PICARD et al, 1990;FERRIE;KELLER, 1995;DEVAUX;PICKERING, 2005 REINERT, 1979, HENRY;BUYSER, 1990;FERRIE;KELLER,1995).…”

Section: Touraev; Heberle-bors 2005)unclassified

“…Segundo Picard et al (1990), isso se deve à sincronização do ciclo celular, bem como a indução da formação de maior números de polens "P" que seriam os mais responsivos à inversão da rota gametofítica para a esporofítica. Em cevada, o pré-tratamento aplicado nos micrósporos isolados interfere na taxa de regeneração de plantas albinas, plantas verdes bem como, na fertilidade das mesmas (HUANG, 1990;LABBANI et al, 2005;OLESZCZUK;ZIMNY, 2006).…”

Section: Pré-tratamento De Frio Das Anterasunclassified

Formação de estruturas androgenéticas por cultura de anteras de trigo em função de doses de nitrogênio e boro aplicadas às plantas doadoras

Michel¹

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Grande), pelo auxilio no desenvolvimento e construção da câmara de crescimento das plantas.Ao meu irmão Flávio Michel pelo auxilio na construção da câmara para cultivo das plantas.Aos professores da ESALQ/USP que participaram do programa DINTER pelos ensinamentos e compreensão ao longo do curso.À secretária da Pós-Graduação em Fitotecnia Luciane Lopes Toledo pelo profissionalismo e solicitude em responder as demandas ao longo do curso. À Secretária da Pós-Graduação em Fisiologia e Bioquímica de Plantas Maria SolizeteGranziol Silva pelo apoio ao longo da estadia na Esalq.À minha esposa Silvania Segati pelo apoio e compreensão ao longo do desenvolvimento do trabalho e pelo auxílio na condução dos experimentos. À empresa Schumacher Insumos para Indústria LTDA, pela doação do substrato (perlita expandida) para a execução dos experimentos. À Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) peloProjeto DINTER e pela concessão de bolsa de estudo durante o desenvolvimento do estágio na ESALQ/USP, em Piracicaba.Enfim, a todos que de uma forma ou de outra contribuíram para a realização desse trabalho.

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Wheat (Triticum aestivum): In Vitro Production and Utilization of Doubled Haploids

Cited by 16 publications

References 40 publications

Performance of isolated microspore-derived doubled haploids of wheat (Triticum aestivum L.)

Performance of isolated microspore-derived doubled haploids of wheat (Triticum aestivum L.)

Genotypic differences in proembryoid development and green plantlets regeneration through androgenesis in barley varieties

Formação de estruturas androgenéticas por cultura de anteras de trigo em função de doses de nitrogênio e boro aplicadas às plantas doadoras

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