Genotypic differences in proembryoid development and green plantlets regeneration through androgenesis in barley varieties

Wiethölter, Paula; Fernandes, M.; Brammer, Sandra Patussi; Minella, Euclydes; Iorkzeski, Edson Jair

doi:10.1590/s0103-84782008000100039

Cited by 2 publications

(3 citation statements)

References 11 publications

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“…In these experiments, the ratio of albino/green plants produced was very high (in particular, for hybrid BG 1-4/98 -79.76%, for genotype BG 367 -100%). Wietholter et al (2008) whereas other researchers (Cistue et al, 2004) also reported 100% ratio of albino/green plants in some barley cultivars [6,7]. On the other hand, in embryogenesis and regeneration frequency there were significant differences between embryoids plated on N6 culture medium and embryoids cultivated on B5 medium.…”

Section: Resultsmentioning

confidence: 91%

Production of wheat doubled haploids by in vitro anther culture

Turasheva

2015

INT J BIOL CHEM

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Production of wheat doubled haploids by in vitro anther cultureThe in vitro culture response of anthers, originating from high yielding and resistance to lodging F 1 soft wheat hybrid plants selected under drought conditions, was investigated. The spikes were collected when the microspores were at the mid-uninucleate stage and after 3 days at 4 0 C for cold pre-treatment, the anthers were cultured in N6 and Gamborg-Eveleg B5 (B5) solid medium. Progeny from 17 different F1 families were used and 9 of them responded to in vitro anther culture and 7 of them produced fertile green plants. Doubled haploid green plants were fertile and produced seeds. Induction of haploid development depends on genotype, stage of male gametophyte development, composition of nutrient media and conditions of cultivation. The effect of the composition of media and different genotypes on wheat anther culture was investigated. From the genotypes produced regenerants, the high yielding genotypes from local breeding collection of wheat produced more albino plants (35.2%) compared to high yielding genotypes from CYMMIT collection (24.92%) ones. Independent of culture medium composition a ratio of doubled haploid/haploid was 4.57:1 and a ratio of haploid/aneuploid was 18.5:1, in particular 81.28 % of green plants were doubled haploid, 17.76% were haploid and the rest (0.96%) were aneuploid

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Section: Resultsmentioning

confidence: 91%

Production of wheat doubled haploids by in vitro anther culture

Turasheva

2015

INT J BIOL CHEM

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“…Em cevada, diversas análises genéticas têm mostrado que a regeneração e a indução estão sob controle de diferentes sistemas poligênicos e são influenciadas por genes com efeitos dominantes, em que um alto número de alelos dominantes refletiria em uma alta resposta à cultura (MARCHETTI et al, 1995;WIETHÖLTER et al, 2008) .…”

Section: Resultsunclassified

“…Assim, após uma geração, obtém-se descendentes homozigotos, altamente desejáveis pelos programas de melhoramento. Na cevada, a cultura de anteras é uma das mais eficientes técnicas para obtenção de haplóides e duplo-haplóides e vários estudos sugerem que alguns fatores genéticos parecem estar envolvidos na capacidade de produção de plantas duplo-haplóide (FAROUGHI-WEHR et al, 1982;LARSEN et al, 1991;WIETHÖLTER et al, 2008). Entretanto, mesmo que a capacidade androgenética esteja associada à presença de genes específicos, o sucesso no desenvolvimento dessas plântulas também depende do estádio fisiológico da planta doadora, do pré-tratamento a frio antes da cultura, do estágio do micrósporo, do meio de cultura, da incubação das anteras e do procedimento de regeneração (OLSEN, 1987;MORAES-FERNANDES et al, 1999).…”

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Desenvolvimento de populações duplo-haplóides de cevada cervejeira associadas à atividade das enzimas (1-3, 1-4)-β-glucanases

et al. 2011

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RESUMO Populações duplo-haplóides apresentam especial vantagem para análises genéticas, uma vez que a informação ABSTRACT Doubled haploid populations offer special advantages in genetic analyses, since the information they provide may be maximized due to the fact that all loci are homozygous. The aim of this study was to develop two barley (Hordeum vulgare ssp.vulgare L.) doubled-haploid (DHs) populations segregating to (1-3, 1-4)--glucanases activity, utilizing the anther culture protocol. Two crosses were performed with contrasting cultivars to (1-3, 1-4)--glucanases activity. Parental cultivars used were 'MN 698' and 'CEV 97047' for the development of 'green malt' population (MV) and Embrapa 127 and 'CEV 96025' for the development of "dry malt" population (MS). For the MS and MV populations, 10,734 and 4,139 anthers were cultured, respectively

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Genotypic differences in proembryoid development and green plantlets regeneration through androgenesis in barley varieties

Abstract: ABSTRACT

Cited by 2 publications

References 11 publications

Production of wheat doubled haploids by in vitro anther culture

Production of wheat doubled haploids by in vitro anther culture

Desenvolvimento de populações duplo-haplóides de cevada cervejeira associadas à atividade das enzimas (1-3, 1-4)-β-glucanases

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