Wheat Lysin-Motif-Containing Proteins Characterization and Gene Expression Patterns under Abiotic and Biotic Stress

Abstract

Lysin motif (LysM)-containing proteins (LYPs) are important pattern recognition receptors in plants. However, the evolutionary history and characteristics of LYP genes remain largely unclear in wheat. In this study, 62 LYPs were identified at genome wide in wheat. Based on phylogenetic and domain analysis, wheat LYPs were classified into 6 subgroups (group LysMe, LysMn, LYP, LYK, LysMFbox). Syntenic analysis showed the evolution of LYP genes in wheat. RNA-seq data showed that 22 genes were not expressed at any tissue or stress stimulation period. Some LYP and LYK genes were tissue-or stage-specific. The majority of TaLYK5s, TaLYK6s, TaLYP2s and TaLysMns genes were induced under chitin, flg22 and fungal treatment. qRT-PCR analysis showed that 4 genes were upregulated during Puccinia triticina infection with a peak at 18 h post inoculation. Our findings suggested that wheat LYPs may have specific roles in response to fungal infection and provided insights into the function and characteristics of wheat LYP genes.

Genome-Wide Identification of the Soybean LysM-RLK Family Genes and Its Nitrogen Response

“…The abundance of these cis-elements in the promoter regions of soybean LysM-RLKs suggests their crucial role in facilitating the plant's adaptation to environmental fluctuations. Moreover, previous studies have explored the responses of LysM genes to biotic and abiotic stresses in wheat and banana [43,44]. However, the functional implications of the candidate LysM-RLK genes, identified via our bioinformatic analysis, in biological and abiotic stress remain uncertain, warranting further investigation and deliberation.…”