Genome-Wide Identification of the Soybean LysM-RLK Family Genes and Its Nitrogen Response

Abstract

Lysin-Motif receptor-like kinase (LysM-RLK) proteins are widely distributed in plants and serve a critical role in defending against pathogens and establishing symbiotic relationships. However, there is a lack of comprehensive identification and analysis of LysM-RLK family members in the soybean genome. In this study, we discovered and named 27 LysM-RLK genes in soybean. The majority of LysM-RLKs were highly conserved in Arabidopsis and soybean, while certain members of subclades III, VI, and VII are unique to soybean. The promoters of these LysM-RLKs contain specific cis-elements associated with plant development and responses to environmental factors. Notably, all LysM-RLK gene promoters feature nodule specificity elements, while 51.86% of them also possess NBS sites (NIN/NLP binding site). The expression profiles revealed that genes from subclade V in soybean roots were regulated by both rhizobia and nitrogen treatment. The expression levels of subclade V genes were then validated by real-time quantitative PCR, and it was observed that the level of GmLYK4a and GmLYK4c in roots was inhibited by rhizobia but induced via varying concentrations of nitrate. Consequently, our findings provide a comprehensive understanding of the soybean LysM-RLK gene family and emphasize the role of subclade V in coupling soybean symbiotic nitrogen fixation and nitrogen response.

Genomic mechanisms of plant growth-promoting bacteria in the production of leguminous crops

“…In addition, genomics also unveil some PGPR which researcher analyze and make comparison of their genetic variation that contribute to plant growth-promotion potential. This knowledge does assist to optimize and choose various microbial strains that contribute to nitrogen fixation (Yao et al, 2023).Biotechnological and novel genomic processes in plants can provide solutions that can reveal the effectiveness of the objectives (Figueiredo et al, 2023). Novel techniques comprising biotechnology and the production of bio-based material can perform the function of improving agricultural sustainability.…”