Wheat biotechnology: A minireview

Khurana, Paramjit; Patnaik, Debasis

doi:10.2225/vol4-issue2-fulltext-4

Cited by 12 publications

(3 citation statements)

References 106 publications

(127 reference statements)

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“…Up till now, several such methods have been developed. Among them, the most often listed ones are 1) infiltration, 2) silicon carbide fiber-mediated transformation, 3) electroporation of cells and tissues, 4) electrophoresis of embryos, 5) microinjection, 6) transformation via the pollentube pathway and 7) liposome-mediated transformation [34,35,40,43,47]. The majority of these were thought to be solutions for the effective transformation of recalcitrant species, such as monocots or some legumes, as Agrobacteriummediated transformation was at that time not available for this group of plants.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: The myth of plant transformation

Rao

Bakhsh

Kiani

et al. 2009

Biotechnology Advances

102

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Section: Introductionmentioning

confidence: 99%

RETRACTED: The myth of plant transformation

Rao

Bakhsh

Kiani

et al. 2009

Biotechnology Advances

102

View full text Add to dashboard Cite

“…It serves as a vital energy source, providing 73% of calories, 7% carbohydrates, 6% dietary fiber, 12% water, 2% fats, 6.2% proteins, and 1.8% minerals. Wheat straw, on the other hand, is employed as animal feed, contributing to increased milk production in cattle, and is also utilized as a roofing material in rural areas, enhancing plaster hardness and toughness (Debasis and Khurana, 2001). Wheat crops exhibit adaptability to both summer and winter conditions, enabling year-round cultivation in many countries, making it the second-largest cultivated cereal crop globally, following rice.…”

Section: Introductionmentioning

confidence: 99%

Identification of Cell Wall Invertase Activities in Selected Wheat Cultivars from Sindh for Crop Improvement

Memon,

Bux,

Samoo

2024

Plant Prot.

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CWI markers, also known as Cell Wall Invertase markers, play a pivotal role in wheat breeding and crop improvement. These markers help identify wheat varieties with desirable traits related to yield potential, stress tolerance, drought tolerance, and overall crop performance. The CWI gene, found in bread wheat, has been identified on chromosomes 4A, 5B, and 5D, all of which contain the conserved WECPDF domain. The main role of the Cell wall invertase (CWI) gene is to hydrolyze sucrose into glucose and fructose. Harnessing the power of CWI markers contributes to the advancement of sustainable agriculture practices and the development of high-performing wheat varieties to meet the demands of diverse agricultural landscapes. In this study, we investigated the prevalence of CWI markers, specifically CWi21 and CWi22, in wheat varieties cultivated in Sindh, Pakistan. We collected a set of thirteen wheat genotypes cultivated in the Sindh province for the screening of Cell Wall Invertase markers through polymerase chain reactions. Our analysis revealed the diversity of the markers CWi21 and CWi22 in wheat genotypes. The marker CWi21 was present in 30% of the wheat varieties, while CWi22 was detected in 70% of the wheat varieties.

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“…Additionally, this approach helps in easy identification of deleterious mutations which are usually in recessive state and remains unidentified in other approaches but in case of haploids that express in hemizygous state, plants can express both dominant and recessive Cereal Research Communications 47, 2019 mutations. Doubled haploidy technology plays an important role in developing transgenics with the desired traits (Patnaik and Khurana 2001;Foster et al 2010;Chauhan and Khurana 2011).…”

Section: Introductionmentioning

confidence: 99%

New protocol for colchicine induced efficient doubled haploidy in haploid regenerants of tetraploid and hexaploid wheats at in vitro level

Sharma

Chaudhary

Manoj

et al. 2019

Cereal Research Communications

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An investigation to standardize the protocol for in vitro application of colchicine for enhancing the doubled haploid production in wheat was done. Two tetraploid (PDW-314 and A-9-30-1); and two hexaploid (DH-40 and C-306) wheat genotypes were used as maternal parents, whereas, the pollen sources involved Zea mays (cv. Bajaura Makka) and Imperata cylindrica. During the rabi seasons of years 2013-14 and 2014-15, wheat × maize and wheat × I. cylindrica hybridization was carried out followed by treatment of their haploids produced as a result of elimination of chromosomes of maize and I. cylindrica respectively, with varied doses of colchicine for different durations The various doses of colchicine were categorized into two groups: lower doses for longer durations (0.01, 0.025, 0.05% each for 5, 7, 9, 11 hrs) and higher doses for shorter durations (0.05, 0.075, 0.10, 0.15, 0.20, 0.25% each for 5, 4, 3, 2 hrs). The response of different concentrations of colchicine applied for varied durations revealed significant differences for various doubled haploidy parameters viz., per cent survived plants, per cent doubled haploid formation and per cent doubled haploid seed formation. In hexaploid and tetraploid wheats, colchicine doses of 0.075% for 4 hrs and 0.15% for 4 hrs, respectively were established as optimum for enhanced doubled haploid production.

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Wheat biotechnology: A minireview

Cited by 12 publications

References 106 publications

RETRACTED: The myth of plant transformation

RETRACTED: The myth of plant transformation

Identification of Cell Wall Invertase Activities in Selected Wheat Cultivars from Sindh for Crop Improvement

New protocol for colchicine induced efficient doubled haploidy in haploid regenerants of tetraploid and hexaploid wheats at in vitro level

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