“…Laboratory diagnosis of dengue can be accomplished by viral isolation from samples of patients, detection of the viral genome, detection of viral antigens, especially the NS1 protein, and serological studies. Basic serological tests have been used for the diagnosis of dengue infection, such as hemagglutination inhibition (HI), complement fixation (CF), the neutralization test (NT), capture and sandwich types of ELISA, and point-of-care (PoC) tests [6,11,12]. Despite the availability of different laboratory techniques for the diagnosis of dengue, some obstacles must be overcome for the development of techniques and new tools, such as obtaining antigens of dengue viruses on a large scale to capture specific antibodies present in suspected sera.…”