Production of Proteins prM/M and E of Dengue Virus-3 in Pichia pastoris: Simplified Purification and Evaluation of Their Use as Antigens in Serological Diagnosis of Dengue
Abstract:Dengue is a major arbovirus affecting humans today. With the growing number of cases, it is essential to have large-scale production of antigens for the development of diagnostic kits for the rapid detection of patients infected by the virus and consequent proper medical intervention for them. In this work, we express the prM/M and E proteins of dengue virus-3 in yeast Pichia pastoris KM71H. The proteins were produced in soluble form in the supernatant of the culture and were purified by precipitation with amm… Show more
“…To develop diagnostic kits for the rapid detection of the virus, it is essential to produce the antigens at large scale. Teixeira et al [25] developed a method to produce the prM/M and E proteins of dengue virus-3 using the yeast Pichia pastoris as an efficient cell factory. The proteins were secreted in the medium of the culture and could subsequently be easily purified by ammonium sulfate precipitation.…”
This Special Issue is a continuation of the first, second, and third “Yeast Biotechnology” Special Issue series of the journal Fermentation (MDPI) [...]
“…To develop diagnostic kits for the rapid detection of the virus, it is essential to produce the antigens at large scale. Teixeira et al [25] developed a method to produce the prM/M and E proteins of dengue virus-3 using the yeast Pichia pastoris as an efficient cell factory. The proteins were secreted in the medium of the culture and could subsequently be easily purified by ammonium sulfate precipitation.…”
This Special Issue is a continuation of the first, second, and third “Yeast Biotechnology” Special Issue series of the journal Fermentation (MDPI) [...]
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.