What is the relationship between the metabolism of preimplantation embryos and their developmental competence?

Barnett, Deborah K.; Bavister, Barry D.

doi:10.1002/(sici)1098-2795(199601)43:1<105::aid-mrd13>3.3.co;2-1

Cited by 29 publications

(38 citation statements)

References 190 publications

(393 reference statements)

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“…NADH can also be reoxidized to regenerate NAD by converting pyruvate to lactate via lactate dehydrogenase (Horton et al, 1996). Elevated levels of glucose have been shown to inhibit the development of embryos in vitro for a number of species including hamster, mouse, cattle, and sheep (for review see, Barnett and Bavister, 1996). In contrast, glucose has been shown to not inhibit embryo development in the pig but rather is necessary for pig embryos to develop to the blastocyst stage in vitro (Petters et al, 1990).…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis of in vivo‐ and in vitro‐produced porcine blastocysts by small amplified RNA‐Serial analysis of gene expression (SAR‐SAGE)

Miles

Blomberg²,

Krisher

et al. 2007

Molecular Reproduction Devel

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Production of embryos in vitro has enormous potential for research and commercial applications. Unfortunately, in vitro production of porcine embryos is extremely inefficient. Despite the characterization of distinct phenotypes, little is known about the molecular mechanisms and altered physiological processes of in vitro-produced embryos. The objective of this study was to compare global gene expression patterns from in vivo- (IVO) and in vitro-produced (IVP) porcine embryos using small amplified RNA-serial analysis of gene expression (SAR-SAGE). Whole-cell RNA from pools of Day 6 IVO and IVP blastocysts was used to construct SAR-SAGE libraries. Sequence analysis of the IVO and IVP libraries yielded 98,771 and 98,408 tags, respectively. A total of 20,029 and 23,453 putative transcripts were detected in the IVO and IVP libraries, respectively. Statistical analyses of SAGE tag frequencies between the IVO and IVP libraries indicated that 938 and 193 tags were differentially expressed at a P < 0.05 and P < 0.001 level of significance, respectively, suggesting significant deviations in transcriptome profiles from IVO and IVP embryos. Categorization of differentially expressed transcripts into functional groupings indicated a significant deviation in gene expression from IVP blastocysts compared with IVO blastocysts for a number of biological processes including cellular metabolism, organization, and response to stress. Real-time PCR confirmed differential expression for several transcripts from independent IVO and IVP blastocysts. These results demonstrate compromised gene expression in IVP blastocysts compared with IVO blastocysts for a number of biological processes, particularly processes involved in mitochondrial function; thereby providing potential target pathways for improvement of IVP methods.

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Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis of in vivo‐ and in vitro‐produced porcine blastocysts by small amplified RNA‐Serial analysis of gene expression (SAR‐SAGE)

Miles

Blomberg²,

Krisher

et al. 2007

Molecular Reproduction Devel

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“…Pyruvate and lactate, in the absence of glucose, are able to support in vitro development of bovine embryos (Kim et al, 1993), glucose, however, is widely used as a supplement and is the major energy substrate in most of the culture media. Moreover, it has been demonstrated that inclusion of glucose in culture media affects negatively the development of early-stage embryos in hamsters (Schini & Bavister, 1988;Barnett & Bavister, 1996;Barnett et al, 1997), mice (Chatot et al, 1989;Lawitts and Biggers, 1991;Scott & Whittingham, 1996), rats (Kishi et al, 1991;Miyoshi et al, 1994), cattle (Kim et al, 1993), sheep (Thompson et al, 1992) and humans (Conaghan et al, 1993;Quinn, 1995). Moreover, it has been demonstrated that inclusion of glucose in culture media affects negatively the development of early-stage embryos in hamsters (Schini & Bavister, 1988;Barnett & Bavister, 1996;Barnett et al, 1997), mice (Chatot et al, 1989;Lawitts and Biggers, 1991;Scott & Whittingham, 1996), rats (Kishi et al, 1991;Miyoshi et al, 1994), cattle (Kim et al, 1993), sheep (Thompson et al, 1992) and humans (Conaghan et al, 1993;Quinn, 1995).…”

Section: Introductionmentioning

confidence: 99%

Supplementation of fructose in chemically defined protein-free medium enhances the in vitro development of bovine transgenic cloned embryos

Bhuiyan

Kang

2007

Zygote

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The present study evaluated the possible embryotrophic role of fructose supplementation in chemically defined protein-free KSOM on in vitro development of bovine transgenic cloned embryos. Bovine fetal fibroblasts transfected with expression plasmids for bovine prion protein (PrP) mutant gene with GFP marker gene were used as donor nuclei for reconstruction of slaughterhouse-derived in vitro matured oocytes. The reconstructed oocytes were cultured in KSOM supplemented with 0.01% PVA (KSOM-PVA) at 39 degrees C in a humidified atmosphere of 5% CO2, 5% O2 and 90% N2 for 192 h. In Experiment 1, when reconstructed oocytes were cultured in KSOM-PVA supplemented with glucose (0.2 mM), fructose (1.5 mM) or combined glucose and fructose (0.2 and 1.5 mM, respectively), significantly (p < 0.05) higher blastocyst (19.2%) and hatching/hatched blastocyst (13.1%) formation rates were obtained in combined fructose and glucose supplemented medium than glucose supplemented counterpart (10.0% and 5.7%, respectively). In Experiment 2, when reconstructed oocytes were cultured in KSOM-PVA supplemented with 0.0, 0.2, 1.5, 3.0 and 5.6 mM fructose in combination with 0.2 mM glucose, the blastocyst formation rate was significantly higher (17.6%) in 1.5 mM fructose supplemented group than that of no fructose supplemented counterpart (9.7%; p > 0.05). In conclusion, supplementation of combined fructose (1.5 mM) and glucose (0.2 mM) in chemically defined protein-free KSOM enhances the in vitro development of bovine transgenic cloned embryos.

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“…A culture medium is a foreign environment for the human embryo. Hence, the design of media is complicated, because the components must be selected, and their concentrations determined in order to minimize stress for the cultured embryo (2,14,15). It also became clear that early embryos show an evolving need for energy substrates, moving from a pyruvatelactate preference -while the embryos are under maternal genetic control -to glucose-based metabolism after activation of the embryonic genome (7,16).…”

Section: Evolution Of Embryo Culture Mediamentioning

confidence: 99%

Embryo culture media for human IVF: which possibilities exist?

Gruber¹,

Klein²

2011

J Turkish German Gynecol Assoc

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The last three decades have seen considerable progress in the development of culture media for ART and infertility treatment. Basic research on the metabolism of mammalian preimplantation embryos demonstrated the specific needs in the evolving stage of the embryo growing in vitro. Two different philosophies led to two different culture strategies for human preimplantation embryos: the 'back-to-nature' or sequential culture principle, and 'let-the-embryo-choose' or one-step culture principle. Both systems are commercially available and the discussion between the different groups of scientists is ongoing. As a matter of fact, all ART culture media currently used are not optimal for the growing human preimplantation embryo. However, further research is needed to reduce stress to the human preimplantation embryo and determine how many embryos from a treatment cycle are capable of producing a live birth.

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What is the relationship between the metabolism of preimplantation embryos and their developmental competence?

Cited by 29 publications

References 190 publications

Comparative transcriptome analysis of in vivo‐ and in vitro‐produced porcine blastocysts by small amplified RNA‐Serial analysis of gene expression (SAR‐SAGE)

Comparative transcriptome analysis of in vivo‐ and in vitro‐produced porcine blastocysts by small amplified RNA‐Serial analysis of gene expression (SAR‐SAGE)

Supplementation of fructose in chemically defined protein-free medium enhances the in vitro development of bovine transgenic cloned embryos

Embryo culture media for human IVF: which possibilities exist?

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