What do we use: Platelet‐rich plasma or platelet‐leukocyte gel?

Everts, Peter A.; Zundert, André van; Schönberger, Jacques P.A.M.; Devilee, Roger J.J.; Knape, J. T. A.

doi:10.1002/jbm.a.31570

Cited by 95 publications

(80 citation statements)

References 11 publications

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“…In fact, several studies have pointed out the importance of leukocytes in PRP 35 for their anti-infectious action 36,37 and immune regulation, 38,39 outlining their capability to produce large amounts of VEGF. 40 At the same time, the presence of some cytokines and the density and composition of the fibrin matrix (which supports their release) should be considered as another key parameter of any platelet concentrate.…”

Section: Discussionmentioning

confidence: 99%

Platelet-Rich Plasma for Androgenetic Alopecia

Schiavone¹,

Raskovic²,

Greco³

et al. 2014

Dermatologic Surgery

126

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Section: Discussionmentioning

confidence: 99%

Platelet-Rich Plasma for Androgenetic Alopecia

Schiavone¹,

Raskovic²,

Greco³

et al. 2014

Dermatologic Surgery

126

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“…The influence of buffy coat including leukocytes is still unclear [21]. Some authors recommend the elimination of leukocytes [22], while several studies have shown that leukocytes in buffy coat play a part in the effects of PRP [23]. In our experiment, the buffy coat layer was eliminated because the aim of this study is to investigate effects of PRP simply depending on different platelet concentrations.…”

Section: Discussionmentioning

confidence: 99%

Effects of Platelet-Rich Plasma on Intestinal Anastomotic Healing in Rats: PRP Concentration is a Key Factor

Yamaguchi

Terashima

Yoneyama

et al. 2012

Journal of Surgical Research

115

114

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“…[17,22] Others ignore the effects of leukocytes or, in other words, the need for the removal of leukocytes in PRP. [23] More importantly, to our knowledge, there are no clinical and/or preclinical studies revealing the amount of leukocyte and platelet content of PRP clearly.…”

Section: Discussionmentioning

confidence: 93%

Does leukocyte-poor or leukocyte-rich platelet-rich plasma applied with biopolymers have superiority to conventional platelet-rich plasma applications on chondrocyte proliferation?

Şirin

Yılmaz

Isyar

et al. 2017

Eklem Hastalik Cerrahisi

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ÖZAmaç: Bu çalışmada trombositten zengin plazma (TZP) içeriğindeki lökosit konsantrasyonunun ve TZP'nin ilaç taşıyıcı sistem kullanılarak uygulanmasının in vitro koşullarda kondrosit proliferasyonu üzerine olası etkileri araştırıldı. Hastalar ve yöntemler: Medikal veya konservatif tedaviye yanıt vermemiş ve total diz artroplastisi geçirmiş dokuz erkek hastanın (ort. yaş 65 yıl; dağılım 49-81 yıl) kanı iki formülasyon hazırlamak üzere kullanıldı: Düşük konsantrasyon lökositli TZP (2000-4000 lökosit/µL) saf TZP (S-TZP) olarak, yüksek konsantrasyon lökositli TZP (9000-11000 lökosit/µL) ise lökositten zengin TZP (L-TZP) olarak belirlendi. Numuneler kontrol grubu (grup 1), direkt S-TZP uygulanan kondrosit kültürleri (grup 2), direkt L-TZP uygulanan kondrosit kültürleri (grup 3), S-TZP uygulanan hidrojel ile kültürlenen kondrositler (grup 4) ve L-TZP uygulanan hidrojel ile kültürlenen kondrositler (grup 5) olmak üzere beş gruba ayrıldı. Tüm gruplarda hücre morfolojisi, canlılık ve proliferasyon bir prekondrosit belirteci olan evreye özgü embriyonik antijen 1'in (SSEA-1) ifadesi ile karşılaştırıldı. Bulgular: Maksimum hücre proliferasyonu ve SSEA-1 ifadesi grup 4'te gerçekleşti; SSEA-1 ifadesi ve hücre proliferasyonu arasında istatistiksel olarak anlamlı ilişki vardı. Sonuç: Çalışmamız TZP'nin lökosit konsantrasyonunun ve hidrojel gibi taşıyıcı sistemlerin önemini ve taşıyıcı sistem ile uygulanan L-TZP'nin kıkırdak hasarının tedavisinde TZP'nin konvansiyonel uygulamalarından daha etkin olduğunu gösterdi.Anahtar sözcükler: Eşkültür teknikleri; hidrojel; trombositten zengin plazma; evreye özgü embriyonik antijenler. ABSTRACTObjectives: This study aims to investigate the possible effects of leukocyte concentration in the content of platelet-rich plasma (PRP) and the administration of PRP using a drug delivery system on chondrocyte proliferation in vitro conditions. Patients and methods: Blood from nine male patients (mean age 65 years; range 49 to 81 years) with advanced stage osteoarthritis who had not responded to medical or conservative treatments and underwent total knee arthroplasty was used to prepare two formulations: PRP with low concentration leukocytes (2000-4000 leukocytes/µL) was designated as pure PRP (P-PRP), whereas PRP with high concentration leukocytes (9000-11000 leukocytes/µL) as leukocyte-rich PRP (L-PRP). Samples were divided into five groups as control group (group 1), chondrocyte cultures with P-PRP applied directly (group 2), chondrocyte cultures with L-PRP applied directly (group 3), chondrocytes co-cultured with P-PRP applied hydrogel (group 4), and chondrocytes co-cultured with L-PRP applied hydrogel (group 5). In all groups; cell morphology, viability and proliferation were compared with the expression of stagespecific embryonic antigen-1 (SSEA-1), a precondrocyte marker. Results: Maximum cell proliferation and SSEA-1 expression occurred in group 4, with a statistically significant correlation between SSEA-1 expression and cell proliferation. Conclusion: Our study showed the importance of leuko...

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What do we use: Platelet‐rich plasma or platelet‐leukocyte gel?

Cited by 95 publications

References 11 publications

Platelet-Rich Plasma for Androgenetic Alopecia

Platelet-Rich Plasma for Androgenetic Alopecia

Effects of Platelet-Rich Plasma on Intestinal Anastomotic Healing in Rats: PRP Concentration is a Key Factor

Does leukocyte-poor or leukocyte-rich platelet-rich plasma applied with biopolymers have superiority to conventional platelet-rich plasma applications on chondrocyte proliferation?

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