What do we know about the structure of human thymic Hassall’s corpuscles? A histochemical, immunohistochemical, and electron microscopic study

Mikušová, Renáta; Mestanova, Veronika; Polák, Štefan; Varga, Ivan

doi:10.1016/j.aanat.2017.02.006

Cited by 20 publications

(12 citation statements)

References 40 publications

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“…Thus, the number of lymphocytes in the cortical area of the thymus lobules of lambs of the second month of age. At the limits of the medullary zone of the lobules of the thymus, the number of lymphocytes in the field of view increases proportionally, rescherches found by (Mikušová et al, 2017), thymic bodies are also defined herein, also the thymocytes of this zone have a morphology of medium and small lymphocytes, have a high degree of differentiation, according to (Palumbo, 2008), in the medullary zone of the thymus, thymocytes undergo antigen-dependent maturation. This area is formed by a dense network of epithelial reticulocytes and its cells are small.…”

Section: Discussionmentioning

confidence: 66%

“…The need to saturate the market of food and industrial raw materials predetermines a further increase herd of livestock, increased productivity and improved product quality. This in turn requires an in-depth knowledge of the structural and functional organization of the organism as a whole and of its different parts, including the organs of the lymphoid system (Mikušová et., 2017).…”

Section: Introductionmentioning

confidence: 99%

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Study of anatomical, histological and cytological characteristics of the thymus of lambs

Rahmoun¹,

Lieshchova²,

Chaanbi³

et al. 2020

TAVM

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Thymus of lambs of the Ouled-Djellal breed from eastern Algeria, aged one to 10 months, were used to study the anatomical, histological and cytological characteristics of these organs. Modern techniques have been used to demonstrate the morphometric parameters and masses of the thymus at different ages, a regression of the morphometric and mass indexes has been visualized according to the age of the lambs. Staining techniques with Hematoxylin and eosin revealed the normal structure of the thymus with its tissue components. The thymus is bilobed. The lobe is surrounded by a thin capsule and subdivided into lobules separated by septae, the cortical and medullary areas were clearly visible, the interlobular blood vessels enter the lobule in all its aspects to continue its way as interlobular blood vessels. From the changes observed in the involution of the thymus according to the age of the animals which occur relatively late, the percentage of the surface found for the capsule was very variable, which increases from the first to the tenth month, the demarcation of the cortex and the medulla beginning during the second month of age of the animal. The interlobular connective tissue (ILC) presents sums, the maximum of which was found in the fourth month of age of the animal, while the minimum was found in the thymus of ninth month lambs Concerning the Hassal corpuscles, their sums are in high extension until the tenth month, rich in T lymphocytes, whose use of immunohistochemistry, the anti-CD 157+ antibody allowed us to visualize to locate them clearly, which invade and form most of the thymus cortex. In conclusion, the significant changes in thymus development in sheep of the Ouled-Djellal breed are similar to those of other species.

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Section: Discussionmentioning

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Study of anatomical, histological and cytological characteristics of the thymus of lambs

Rahmoun¹,

Lieshchova²,

Chaanbi³

et al. 2020

TAVM

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“…This homologous structure to the mouse keratinocyte-like mTEC is Hassall's corpuscle (HC), which are distinct, closely packed, fully keratinized swirls of cells that contain filaggrin. 85,86 Notably, already in 1989, one study proposed that HCs are terminally differentiated mTEC 87 and this was supported in a recent study, 88 although their development is still considered a mystery. HCs have previously been considered rare or non-existing in mice 89 or described as small.…”

Section: Keratinocyte-like Mtec and The Role Of These In γδ T Cell mentioning

confidence: 91%

γδ T cells and inflammatory skin diseases

Jee

Mraz

Geisler

et al. 2020

Immunological Reviews

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γδ and αβ T cells develop in the thymus from common progenitor cells. 1 Unlike αβ T cells, the majority of murine γδ T cells are functionally committed during intra-thymic differentiation. 2-4 This underlines the importance of the thymic microenvironment in the regulation of the peripheral effector functions of γδ T cells. Central to the thymic microenvironment is the thymic epithelial cells (TEC), divided into cortical TEC (cTEC) and medullary TEC (mTEC). 5 Particularly, the mTEC seem to regulate the functional commitment of γδ T cells within the thymus. Recently, it has become clear that TEC, especially mTEC, are more heterogeneous than previously assumed. Interestingly, subsets of mTEC with expression profiles such as tuft cells and keratinocytes (KC) have been identified. 6,7 KC constitute the majority of cells within the outermost layer of the skin, the epidermis. The epidermis acts as our first line of defense against assaults from pathogens, chemicals, and allergens, thereby playing a critical role in providing protection from injury. Although KC are not classified as immune cells, they play important immunological roles in the first line of defense against pathogens and allergens. Thus, KC express pattern recognition receptors such as Toll-like receptors (TLR) 8 and NOD-like receptors (NLR), 9 some constitutively and others following stimulation, that are able to recognize pathogen-associated molecular patterns and damage-associated molecular patterns, which trigger the production and release of cytokines, chemokines, and anti-microbial peptides that in turn activate immune cells in the skin. 10-12 The immune cells found in the epidermis are T cells and a subset of antigen-presenting cells termed Langerhans cells (LC). 10,12,13 In naïve mice, the primary subset of T cells in the epidermis is a special subset of γδ T cells called dendritic epidermal T cells (DETC) due to their dendritic morphology. 14

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“…The total area of the thymus lobe and the area of the medulla were counted. The presence of Hassall's corpuscules, which are heterocellular, consisting of thymic epithelial cells (major cellular component), macrophages, interdigitating dendritic cells, myoid cells, and, occasionally, mast cells and lymphocytes [28] was evaluated and were counted in the medulla. The data are presented as the median per mm 2 of thymus medulla in each group.…”

Section: Methodsmentioning

confidence: 99%

Gender-Related Effect of Sodium Dichloroacetate on the Number of Hassall’s Corpuscles and RNA NKCC1 Expression in Rat Thymus

Stanevičiūtė

Juknevičienė

Balnytė

et al. 2019

BioMed Research International

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The aim was to investigate the effect of dichloroacetate (DCA) on thymus weight, Hassall’s corpuscle number (HCs), and NKCC1 RNA expression in Wistar rats aged 4–5 weeks. They were investigated in the controls and DCA-treated gonad-intact and castrated males and females. The treatment lasted 4 weeks with DCA 200 mg/kg/day. At the end of the experiment, rat thymus was weighted, and its lobe was taken for the expression of NKCC1 RNA determined by the PCR method and of Hassall’s corpuscles by immunohistochemistry. DCA caused a thymus weight decrease in DCA-treated gonad-intact rats of both genders as compared with their controls (p < 0.05), and no such impact was found in castrated DCA-treated males and females. DCA caused an increase of the HCs in gonad-intact males (p < 0.05), and no such increase in the DCA-treated gonad-intact females was found. There was gender-related difference in the HCs when comparing DCA-treated gonad-intact males and females: males showed significantly higher HCs (p < 0.05); no gender-related differences were found in the castrated DCA-treated groups. The Slc12a2 gene RNA expression level was found to be significantly decreased only in gonad-intact and in castrated DCA-treated males. The authors discuss the gender-related DCA effects on the thymus.

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What do we know about the structure of human thymic Hassall’s corpuscles? A histochemical, immunohistochemical, and electron microscopic study

Cited by 20 publications

References 40 publications

Study of anatomical, histological and cytological characteristics of the thymus of lambs

Study of anatomical, histological and cytological characteristics of the thymus of lambs

γδ T cells and inflammatory skin diseases

Gender-Related Effect of Sodium Dichloroacetate on the Number of Hassall’s Corpuscles and RNA NKCC1 Expression in Rat Thymus

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