What are the prospects for genetically engineered, disease resistant plants?

Collinge, David B.; Lund, Ole; Thordal‐Christensen, Hans

doi:10.1007/s10658-007-9229-2

Cited by 66 publications

(30 citation statements)

References 98 publications

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“…Some other chitinases do not show any antifungal activities (Taira et al, 2005). Chitinases also respond to abiotic stress either in growth or developmental processes (Collinge et al, 1993;Kasprzewska, 2003;Collinge et al, 2008;Sharma et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and expression of drought-induced protein 3 (DIP3) encoding a class III chitinase in upland rice

Guo

Bai²,

Su³

et al. 2013

Genet. Mol. Res.

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ABSTRACT.A drought-induced gene, DIP3, encoding a chitinase III protein was isolated from the roots of upland rice by reverse transcriptionpolymerase chain reaction (RT-PCR). Sequence analysis demonstrated that the cDNA and deduced protein showed high identity to Oryza sativa class III chitinase. The deduced protein contained a signal peptide sequence in the N-terminal region of 21aa and a conserved glycosyl hydrolase (GH) 18 domain. The secondary and 3D structures were analyzed and showed that it contained α-helix, β-sheets, extended strand and random coil structures and that it was approximately spheroidal. Real-time quantitative PCR analysis revealed that expression levels accumulated rapidly under different forms of abiotic stress (drought, salt and low temperature), peaked at different times and then decreased. These results implied that as a member of class III chitinases, DIP3 may function as a stress-induced protein involved in the regulation of plant stress response.

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Section: Introductionmentioning

confidence: 99%

Molecular cloning and expression of drought-induced protein 3 (DIP3) encoding a class III chitinase in upland rice

Guo

Bai²,

Su³

et al. 2013

Genet. Mol. Res.

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“…The low levels of pathogenic resistance by some transgenic crops coupled with the negative perception of genetically modified plants have resulted in a relatively small number of transgenic lines being brought to late stage field testing and even fewer that have been successfully brought to market. With the exception of virus-resistant plants, currently there are no commercially available transgenic plant species with increased resistance towards fungal and bacterial pathogens [87].…”

Section: Genetic Engineering For Pathogen Resistancementioning

confidence: 99%

“…So far, many transformation strategies have been used to increase resistance of crop plants against bacterial, fungal and viral pathogens including: introgressing R genes, introducing genes coding for antimicrobial compounds (chitinase or glucanase enzymes that break down fungal cell walls-chitin or glucan respectively), up regulating defense pathways (through promoter transfer), disarming host susceptibility genes, detoxifying pathogen virulence factors (toxins), increasing structural barriers and silencing essential pathogen genes (RNA silencing, RNA interference, or RNAi) [4,85,87]. For instance, Zhou et al (2009) introgressed two R genes (Xa23 and Rxo1) to develop rice cultivars resistant to bacterial blight and bacterial streak diseases.…”

Section: Genetic Engineering For Pathogen Resistancementioning

confidence: 99%

Identification, Mapping and Pyramiding of Genes/Quantitative Trait Loci (QTLs) for Durable Resistance of Crops to Biotic Stresses

Mekonnen¹,

Haileselassie²,

Tesfaye³

2017

J Plant Pathol Microbiol

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Biotic stresses significantly limit global crop production. Identification and use of resistant cultivars is currently seen as the best strategy, cheapest, durable and environmentally friendly method to manage biotic stresses. However, resistance gained through single gene/quantitative trait loci (QTLs) transfer leads to resistance breakdown within a short period. Hence, current breeding programs targeted at developing durable and/ broad spectrum resistant cultivars by pyramiding multiple resistant genes/QTLs. Despite its significant contributions to crop improvement, gene pyramiding through conventional breeding suffers from being laborious, time consuming, costly and less efficient. Recently, the use of modern molecular tools like molecular markers and genetic engineering has dramatically enhanced the gene pyramiding strategy for biotic stress resistance. Molecular markers are very helpful for precise identification, mapping and introgression of multiple desirable genes/QTLs underlying trait of interest. Moreover, Genetic engineering has enabled scientists to transfer novel genes from any source into plants in a single generation to develop cultivars with the desired agronomic traits. Therefore, the current paper targeted to review the different types of biotic stress resistance in plants and the methodologies for identification, mapping and pyramiding of resistance genes/QTLs to develop durable and/or broad spectrum biotic stress resistant cultivars. So far, numerous crops with durable/broad spectrum resistance to pathogens, insect pests and herbicides have been developed by pyramiding multiple resistant genes/QTLs using marker assisted selection and genetic engineering techniques to contribute to increased crop production and productivity to maintain food security globally.

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“…Recently, trans-plastomic Nicotiana benthamiana plants expressing important defense proteins in three-stacking combinations to obtain multiple resistance (insects, phytopathogens and abiotic stress) had synergistic and enhanced effects in comparison to plants expressing such genes individually (Chen et al, 2014). Single genes expression is particularly prone to rapid breakdown necessitating strategies, such as transgene stacking, to obtain not only higher but also more durable resistance (Collinge et al, 2008;Zhao et al, 2003;Datta et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Expressing stacked HRAP and PFLP genes in transgenic banana has no synergistic effect on resistance to Xanthomonas wilt disease

Abubaker

Tripathi

Kunert

et al. 2016

South African Journal of Botany

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Highlights• Stacked HRAP and PFLP transgenic plants had higher resistance to Xcm than nontransgenic plants.• Cumulative transcripts of both HRAP and PFLP in stacked transgenic did not correlate with resistance.• Transgenic plants accumulated more H 2 O 2 than non-transgenic plants in response to Xcm infection.• The response of transgenic plants to Xcm infection was characterized by up-regulation of genes encoding PR3 and GST.• Stacked HRAP and PFLP had no higher resistance against Xcm in comparison to individual transgenic plants. AbstractBanana production in Africa's great lakes region is threatened by the Banana Xanthomonas however, stacking might provide the benefit of durable resistance in case one transgene function is lost.

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What are the prospects for genetically engineered, disease resistant plants?

Cited by 66 publications

References 98 publications

Molecular cloning and expression of drought-induced protein 3 (DIP3) encoding a class III chitinase in upland rice

Molecular cloning and expression of drought-induced protein 3 (DIP3) encoding a class III chitinase in upland rice

Identification, Mapping and Pyramiding of Genes/Quantitative Trait Loci (QTLs) for Durable Resistance of Crops to Biotic Stresses

Expressing stacked HRAP and PFLP genes in transgenic banana has no synergistic effect on resistance to Xanthomonas wilt disease

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