Identification, Mapping and Pyramiding of Genes/Quantitative Trait Loci (QTLs) for Durable Resistance of Crops to Biotic Stresses

Mekonnen, Tilahun; Haileselassie, T; Tesfaye, Kassahun

doi:10.4172/2157-7471.1000412

Cited by 5 publications

(3 citation statements)

References 63 publications

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“…To respond to fast evolution of a pathogen, a breeder should have access to wide pool of R genes and tools for their fast introduction to breeding lines. Genetic engineering would extend possibilities of gene pyramiding as it allows transferring gene of interest from any source, regardless of crossing barriers, into a crop plant (Mekonnen et al 2017). Recent advances in development of homozygous potato inbred lines are also promising and provide another approach for generating late blight resistant diploid hybrids.…”

Section: Discussionmentioning

confidence: 99%

Marker-assisted pyramiding of potato late blight resistance genes Rpi-rzc1 and Rpi-phu1 on di- and tetraploid levels

et al. 2020

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Late blight is a disease with the biggest economic impact on potato cultivation worldwide. Pyramiding of the resistance genes originating from potato wild relatives is a breeding strategy that has a potential to produce potato cultivars durably resistant to late blight. Growing such cultivars would allow limiting the intensive chemical control of the disease. The goal of this work was to transfer the late blight resistance gene Rpi-rzc1 from Solanum ruiz-ceballosii to the tetraploid level of cultivated potato and to pyramid it with the Rpi-phu1 gene. We obtained two diploid and, through 4x-2x cross, a tetraploid potato population segregating for the Rpi-rzc1 presence, as well as one diploid and one tetraploid population where both genes were introgressed. In total, 754 progeny clones were tested for resistance to late blight in detached leaflet assays. Pathogen isolates avirulent on plants with both genes and virulent on plants with the Rpi-phu1 were used. The selection was assisted by two PCR markers flanking the Rpi-rzc1 gene and a newly designed, highly specific intragenic marker indicating the Rpi-phu1 gene presence. We obtained 26 diploid and 49 tetraploid potato clones with pyramid of both genes that should enhance the durability and spectrum of their late blight resistance and that can be exploited in potato breeding. The specificity of the marker for the Rpi-phu1 gene and the precision of the Rpi-rzc1 mapping were improved in this work.

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Section: Discussionmentioning

confidence: 99%

Marker-assisted pyramiding of potato late blight resistance genes Rpi-rzc1 and Rpi-phu1 on di- and tetraploid levels

et al. 2020

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“…The most efficient strategy to manage harmful pests is enhancing the host plant's resistance to these pathogenic organisms [37]. Therefore, breeding toward durable resistance against a broad spectrum of these pests is the most practical approach for preventing crop loss [38] 9 and 11 for BL resistance were identified in a previous study [20]. The major QTLs on chromosomes 9 and 11 were located in the Pi2/9 [45] and Pik/Pikm [46] regions, respectively.…”

Section: Discussionmentioning

confidence: 99%

Identification of QTLs for Blast, Bacterial Blight, and Planthopper Resistance Using SNP-Based Linkage Maps from Two Recombinant Inbred Rice Lines

Jairin¹,

Vejchasarn²,

Somjai³

et al. 2019

AJPS

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Rice is the most significant global food security. Several biotic factors limit rice production, breeding biotic-resistant rice has, therefore, become an increasingly important goal. Two elite rice lines, IR71033-121-15 (IR71033) and IR57514-PMI-5-B-1-2 (IR57514), provide potential genes for biotic stress resistance traits. In this study, genotyping by sequencing (GBS) for single nucleotide polymorphism (SNP)-based linkage map construction was used to detect quantitative trait loci (QTLs) for blast (BL), bacterial blight (BB), whitebacked planthopper (WBPH), and brown planthopper (BPH) resistance. IR71033 was derived from Oryza minuta and carried BL, BB, WBPH, and BPH resistance QTLs. IR57514 is a well-adapted rainfed lowland line that carries BL and BB resistance QTLs. Two sets of recombinant inbred line (RIL) populations derived from crosses of KDML105 × IR71033 and KDML105 × IR57514 were used to dissect the genetic basis of disease and insect pest resistance. The RIL populations were evaluated for BL, BB, WBPH, and BPH resistance from 2016 to 2018 at four rice research centers in Thailand. From these, we identified a large number of SNPs through GBS and constructed high-resolution linkage maps. By combining phenotypic evaluation with the GBS data, a total of 24 QTLs on four chromosomes were detected that confered pest resistance and explained 7.3%-61.

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“…Reliable biotechnological method have also be developed for identification of GM crops for biosafety and legitimacy of transgenes (Fraiture et al, 2015;Lin and Pan 2016;Nazir et al, 2019). Mekonnen et al, (2017); ISAAA (2018); Nazir et al, (2019) On the contrary, concerns over the lack of knowledge on the safety of GM crops are used as arguments to ban them, or at least to regulate them heavily. Many citizens, politicians and countries generally take those arguments to reinforce misconceptions about GM crop.…”

Section: Concerns and Controversies On The Safety/risks Of Gmomentioning

confidence: 99%

Trend of Biotechnology Applications in Pest Management: A Review

Alemu

2020

Int J Appl Sci Biotechnol

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There are many constraints that severely affect the global agricultural production and productivity which include the ever increasing of population growth, degradation of natural resources, climate changes and emerging pests. Among these factors, biotic constraints or pests are known to cause 25-50% or complete loss of plant production. Accordingly, various plant protection technologies have been deployed with the trend of focusing on the use modern biotechnological tools that are proven to be most effective and mandatory. The review covers a wide array of pest management methods ranging from the conventional biological control methods up to molecular breeding techniques. Furthermore, the application of new genetic engineering techniques fueled by new breakthroughs and innovations are the cornerstone of this review. Accordingly, the continuous increasing trend of GM crops cultivation in both crop type and hectare has urged many countries to deploy the technology as a key strategy to enhance their bioeconomy. In fact, plant protection is the discipline that immensely benefit from biotechnology than any other disciplines for ensuring food security and sustainable development. However, in order to fully exploit the enormous potential of biotechnology, appropriate biosafety regulatory frameworks and proper stewardship programs need to be effectively implemented. This integrated approach can promptly help respond to the ever-dynamic threat of pests and hence reliably combat food insecurity and ably contribute to sustainable development. Int. J. Appl. Sci. Biotechnol. Vol 8(2): 108-131

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Identification, Mapping and Pyramiding of Genes/Quantitative Trait Loci (QTLs) for Durable Resistance of Crops to Biotic Stresses

Cited by 5 publications

References 63 publications

Marker-assisted pyramiding of potato late blight resistance genes Rpi-rzc1 and Rpi-phu1 on di- and tetraploid levels

Marker-assisted pyramiding of potato late blight resistance genes Rpi-rzc1 and Rpi-phu1 on di- and tetraploid levels

Identification of QTLs for Blast, Bacterial Blight, and Planthopper Resistance Using SNP-Based Linkage Maps from Two Recombinant Inbred Rice Lines

Trend of Biotechnology Applications in Pest Management: A Review

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