Western blot analysis of sera from Lyme borreliosis patients according to the genomic species of theBorrelia strains used as antigens

Assous, Marc V.; Postić, D.; Paul, G.; Névot, P; Baranton, Guy

doi:10.1007/bf01967256

Cited by 160 publications

(118 citation statements)

References 32 publications

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“…The genetic distances that separate these groups are consistent with data that were obtained by using a different collection of strains and a smaller number of enzyme loci (15). As reported previously, B. burgdorferi sensu stricto, B. garinii, and B. afzelii are preferentially associated with different chronic manifestations of Lyme disease (7,9,47). Briefly, it appears that the first symptom of the disease, erythema chronicum migrans, is associated with all three of these species, whereas arthritis and acrodermatitis chronica atrophicans are caused by B. burgdorferi sensu stricto and B. afzelii, respectively, and neuroborreliosis occurs after infection by B. garinii or B. burgdo$eri sensu stricto.…”

Section: Discussionsupporting

confidence: 71%

“…Several groups of workers, including our group, have demonstrated that there is an association between these three species and the different chronic manifestations of Lyme disease. B. afzelii and B. gan'nii have been found to be associated with acrodermatitis chronica atrophicans and neuroborreliosis, respectively, and arthritis, which is the predominant late complication of the disease in the United States, has been found to be associated with B. burgdofen' sensu stricto (7,9,47).…”

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confidence: 99%

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Analysis of the Genetic Polymorphism of Borrelia burgdorferi Sensu Lato by Multilocus Enzyme Electrophoresis

Balmelli¹,

Piffaretti²

1996

International Journal of Systematic Bacteriology

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In recent years, Borrelia burgdorferi sensu lato has been subdivided into three species, Borrelia burgdorferi sensu stricto, Borrelia gurinii, and Borrelia afzelii, and a new species restricted to Japan, Borrelia juponica, has been isolated from Ixodes ovatus. In addition, members of several new genomic groups have been found in America and in Europe, suggesting that there are additional genospecies. In order to study the diversity of B. burgdorferi sensu lato, we analyzed 54 isolates cultured from humans and from different tick species and obtained from diverse geographic areas, including Europe, the United States, Japan, and the People's Republic of China. In order to investigate the genetic relationship between microorganisms that are transmitted by soft ticks and microorganisms that cause Lyme disease, we also included three strains of relapsing fever spirochetes. The method which we used was multilocus enzyme electrophoresis; 12 genetic loci were characterized on the basis of the electrophoretic mobilities of their products, and 50 distinct allele profiles (electrophoretic types) were distinguished. The mean genetic diversity per locus was 0.747. A cluster analysis of a matrix of genetic distances for pairs of electrophoretic types revealed 11 divisions that were separated at genetic distances greater than 0.65. Five of these divisions corresponded to B. burgdorferi sensu stricto, B. gurinii, B. afzelii, B. juponica, and the newly proposed species "Borrelia andersonii." Our results also confirmed that there are two additional genomic groups in Europe and at least one additional group in the United States. The relapsing fever spirochetes were not clearly separated from the spirochetes associated with Lyme disease. In conclusion, we believe that the previously proposed subdivision of B. burgdorferi sensu lato into only four species should be reconsidered.

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Section: Discussionsupporting

confidence: 71%

mentioning

confidence: 99%

Analysis of the Genetic Polymorphism of Borrelia burgdorferi Sensu Lato by Multilocus Enzyme Electrophoresis

Balmelli¹,

Piffaretti²

1996

International Journal of Systematic Bacteriology

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“…There are differences in the antigen profile between various species of LB spirochetes that are specifically revealed in the molecular interactions of pathogen with molecular factors (receptors) of tick or host inner environment. Due to the above-mentioned molecular differences significant diversities exist in the LB clinical picture (Assous et al 1993, van Dam et al 1993. Prevalence rates of B. burgdorferi sensu lato in host-seeking Ixodes ricinus ticks in Europe were estimated by Hubálek and Halouzka (1998).…”

Section: Genospecies Of Borrelia Burgdorferi Sensu Latomentioning

confidence: 99%

Lyme borreliosis: insights into tick- / host-borrelia relations

Grubhoffer¹,

Golovchenko²,

Vancová³

et al. 2005

FOLIA PARASIT

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Abstract. Lyme borreliosis (LB) is a serious infectious disease of humans and some domestic animals in temperate regions of the Northern Hemisphere. It is caused by certain spirochetes in the Borrelia burgdorferi sensu lato (s.l.) species complex. The complex consists of 11 species (genospecies). Borrelia burgdorferi sensu stricto (s.s.), Borrelia garinii and Borrelia afzelii are the major agents of human disease. Borrelia burgdorferi s.l. species are transmitted mainly by ticks belonging to the Ixodes ricinus species complex plus a few additional species not currently assigned to the complex. B. burgdorferi infections may produce an acute or chronic disease with a wide array of clinical symptoms such as erythema migrans (EM), carditis, arthritis, neuroborreliosis, and acrodermatitis chronica atrophicans (ACA). Differences in LB spirochetes 'genospecies' and strains/isolates determine the occurrence and severity of this multi-system disease. Accurate and reliable identification of the LB spirochetes in ticks as well as knowledge of their prevalence are essential for prevention against the disease and development of an effective vaccine. An overview of the knowledge of molecular factors with emphasis on potential protein-carbohydrate interactions in the tickborrelia system is the main focus of this review.

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“…In Europe, at least three genospecies are recognized as human pathogens: B. burgdorferi sensu stricto, B. garinii and B. afzelii (Assous et al 1993, Van Dam et al 1993, Wang et al 1999. Depending on the geographical location, various species of the hard-bodied (ixodid) ticks transmit B. burgdorferi s.l.…”

mentioning

confidence: 99%

Effect of salivary gland extract from Ixodes ricinus ticks on the proliferation of Borrelia burgdorferi sensu stricto in vivo

Machácková

Oborník²,

Kopecký³

2006

FOLIA PARASIT

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Abstract. Saliva-activated transmission (SAT) of Borrelia burgdorferi sensu stricto was demonstrated using real-time PCR and salivary gland extract (SGE) from partially fed Ixodes ricinus ticks. C3H/HeN mice were injected intradermally with 1.5 × 10 3 spirochetes mixed with 40 µg of SGE per mouse. The control group was inoculated with the same dose of spirochetes without SGE. The accelerating effect of SGE on spirochete proliferation was demonstrated on day 1 post infection, when a 4.2-fold increase in spirochetes was found in the skin and a 10-fold increase in the blood, compared with control mice. The data represent the first direct evidence of a SAT effect of I. ricinus SGE on infection with the Lyme disease agent B. burgdorferi.

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Western blot analysis of sera from Lyme borreliosis patients according to the genomic species of theBorrelia strains used as antigens

Cited by 160 publications

References 32 publications

Analysis of the Genetic Polymorphism of Borrelia burgdorferi Sensu Lato by Multilocus Enzyme Electrophoresis

Analysis of the Genetic Polymorphism of Borrelia burgdorferi Sensu Lato by Multilocus Enzyme Electrophoresis

Lyme borreliosis: insights into tick- / host-borrelia relations

Effect of salivary gland extract from Ixodes ricinus ticks on the proliferation of Borrelia burgdorferi sensu stricto in vivo

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