West Nile Virus Equine Serosurvey in the Czech and Slovak Republics

Hubálek, Zdeněk; Ludvíková, E.; Jahn, P.; Treml, F.; Rudolf, Ivo; Svobodová, Petra; Šikutová, Silvie; Betášová, Lenka; Bíreš, J.; Mojžíš, Miroslav; Tinák, M.; Boldižár, Martin; Citsoňová, Gabriela; Staššíková, Zuzana

doi:10.1089/vbz.2012.1159

Cited by 17 publications

(18 citation statements)

References 28 publications

(30 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The WNV seroprevalence rate in horses reported in this study is similar or higher than in neighboring countries. For example, in Slovak Republic, the reported seropositivity was 8.3% in 2008–2011, 4.8% in 2012 and 0% in 2013 [ 42 , 43 ]. However, a higher proportion of seropositive horses (13.5%) was detected in 2010–2011 in Ukraine [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

A Survey on West Nile and Usutu Viruses in Horses and Birds in Poland

Bażanów

Vuren

Szymański³

et al. 2018

Viruses

View full text Add to dashboard Cite

West Nile virus (WNV) and Usutu virus (USUV) are members of the family Flaviviridae which, natural life cycles involve mosquito–bird–mosquito transmission. Both represent emerging viruses in Europe with potential to cause neuroinvasive disease in humans. This study investigates the seroprevalence of serum neutralizing antibodies to WNV and to USUV in birds and in horses in Poland. Antibodies against WNV and USUV were detected in 5 (35.7%) and in 1 (7.14%) of 14 birds and in 62 (15.08%) and in 115 (27.98%) of 411 horses, respectively. Twenty-one WNV serologically positive horses (33.87%) and 67 USUV serologically positive horses (58.26%) did not travel outside Polish borders. Given the high abundance of potentially competent mosquito species in Poland, high populations of horses and different bird species, our findings highlight implementation of active control programs, including monitoring of geographic spread and dynamics of WNV and USUV transmission in both primary and accidental hosts. It is also important to improve public health awareness about the disease these viruses may cause.

show abstract

Section: Discussionmentioning

confidence: 99%

A Survey on West Nile and Usutu Viruses in Horses and Birds in Poland

Bażanów

Vuren

Szymański³

et al. 2018

Viruses

View full text Add to dashboard Cite

show abstract

“…In horses, infection with WNV and JEV can lead to severe neurological disease and death, while USUV, SLEV and TBEV infection are mainly asymptomatic but do induce antibody responses (Hubálek et al., ). Horses often serve as sentinels for active virus circulation in serological surveillance programmes specifically for WNV, USUV and JEV (Lupulovic et al., ; Barbic et al., ; Hubalek et al., ; Silva et al., ).…”

Section: Introductionmentioning

confidence: 99%

A Serological Protein Microarray for Detection of Multiple Cross-Reactive Flavivirus Infections in Horses for Veterinary and Public Health Surveillance

Cleton

Maanen

Bergervoet

et al. 2016

Transbound Emerg Dis

View full text Add to dashboard Cite

The genus Flavivirus in the family Flaviviridae includes some of the most important examples of emerging zoonotic arboviruses that are rapidly spreading across the globe. Japanese encephalitis virus (JEV), West Nile virus (WNV), St. Louis encephalitis virus (SLEV) and Usutu virus (USUV) are mosquito-borne members of the JEV serological group. Although most infections in humans are asymptomatic or present with mild flu-like symptoms, clinical manifestations of JEV, WNV, SLEV, USUV and tick-borne encephalitis virus (TBEV) can include severe neurological disease and death. In horses, infection with WNV and JEV can lead to severe neurological disease and death, while USUV, SLEV and TBEV infections are mainly asymptomatic, however, and induce antibody responses. Horses often serve as sentinels to monitor active virus circulation in serological surveillance programmes specifically for WNV, USUV and JEV. Here, we developed and validated a NS1-antigen protein microarray for the serological differential diagnosis of flavivirus infections in horses using sera of experimentally and naturally infected symptomatic as well as asymptomatic horses. Using samples from experimentally infected horses, an IgG and IgM specificity of 100% and a sensitivity of 95% for WNV and 100% for JEV was achieved with a cut-off titre of 1 : 20 based on ROC calculation. In field settings, the microarray identified 93-100% of IgG-positive horses with recent WNV infections and 87% of TBEV IgG-positive horses. WNV IgM sensitivity was 80%. Differentiation between closely related flaviviruses by the NS1-antigen protein microarray is possible, even though we identified some instances of cross-reactivity among antibodies. However, the assay is not able to differentiate between naturally infected horses and animals vaccinated with an inactivated WNV whole-virus vaccine. We showed that the NS1-microarray can potentially be used for diagnosing and distinguishing flavivirus infections in horses and for public health purposes within a surveillance setting. This allows for fast, cheap, syndrome-based laboratory testing for multiple viruses simultaneously for veterinary and public health purposes.

show abstract

“…The above rare traces of WNV infections in the Czech Republic before 2008 were most likely due to WNV lineage 1. Sera collected from 163 horses, originating from 43 out of 77 administrative districts of the Czech Republic between 2008 and 2011, all proved negative for WNV antibodies [12]. Because of the rapidly changing epidemiological situation regarding WNF in Europe, we decided to perform virological surveillance of mosquitoes for WNV and related pathogenic flaviviruses (e.g.…”

Section: Introductionmentioning

confidence: 99%

West Nile virus lineage 2 isolated from Culex modestus mosquitoes in the Czech Republic, 2013: expansion of the European WNV endemic area to the North?

et al. 2014

View full text Add to dashboard Cite

We report the detection and isolation of four almost identical strains of West Nile virus (WNV) lineage 2 from Culex modestus mosquitoes collected at three fish ponds in South Moravia, Czech Republic, during August 2013. Phylogenetic analysis demonstrated that the Czech WNV strains isolated are closely related to Austrian, Italian and Serbian strains reported in 2008, 2011 and 2012, respectively. Our findings show the current northernmost range of lineage 2 WNV in Europe.

show abstract

West Nile Virus Equine Serosurvey in the Czech and Slovak Republics

Cited by 17 publications

References 28 publications

A Survey on West Nile and Usutu Viruses in Horses and Birds in Poland

A Survey on West Nile and Usutu Viruses in Horses and Birds in Poland

A Serological Protein Microarray for Detection of Multiple Cross-Reactive Flavivirus Infections in Horses for Veterinary and Public Health Surveillance

West Nile virus lineage 2 isolated from Culex modestus mosquitoes in the Czech Republic, 2013: expansion of the European WNV endemic area to the North?

Contact Info

Product

Resources

About