Well-Defined Peapod-like Magnetic Nanoparticles and Their Controlled Modification for Effective Imaging Guided Gene Therapy

Abstract

Due to their unique properties, one-dimensional (1D) magnetic nanostructures are of great significance for biorelated applications. A facile and straightforward strategy to fabricate 1D magnetic structure with special shapes is highly desirable. In this work, well-defined peapod-like 1D magnetic nanoparticles (Fe3O4@SiO2, p-FS) are readily synthesized by a facile method without assistance of any templates, magnetic string or magnetic field. There are few reports on 1D gene carriers based on Fe3O4 nanoparticles. BUCT-PGEA (ethanolamine-functionalized poly(glycidyl methacrylate) is subsequently grafted from the surface of p-FS nanoparticles by atom transfer radical polymerization to construct highly efficient gene vectors (p-FS-PGEA) for effective biomedical applications. Peapod-like p-FS nanoparticles were proven to largely improve gene transfection performance compared with ordinary spherical Fe3O4@SiO2 nanoparticles (s-FS). External magnetic field was also utilized to further enhance the transfection efficiency. Moreover, the as-prepared p-FS-PGEA gene carriers could combine the magnetic characteristics of p-FS to well achieve noninvasive magnetic resonance imaging (MRI). We show here novel and multifunctional magnetic nanostructures fabricated for biomedical applications that realized efficient gene delivery and real-time imaging at the same time.

Smart Delivery Systems Based on Poly(glycidyl methacrylate)s‐Coated Organic/Inorganic Core–Shell Nanohybrids

“…Schematic illustration of the fabrication process of peapod‐like Fe 3 O 4 @SiO 2 @PGEA nanohybrids for effective imaging‐guided GT. Reproduced with permission . Copyright 2016, American Chemical Society.…”

“…In order to promote both cellular uptake and transfection activity, Xu and Zhao et al grafted PGEA onto the surface of rare peapod-like one-dimensional magnetic nanoparticles (Fe 3 O 4 @SiO 2 , p-FS) as gene carriers via ATRP method (Figure 9). [54] Furthermore, Fe 3 O 4 @SiO 2 @PGEA possesses excellent performance in noninvasive MRI and the transfection efficiency can be further enhanced by external magnetic field. Thus, real-time imaging guided high efficient GT can be realized by integrating MRI and magnetofection function of 1D peapod-like magnetic nanoparticles.…”

Elongated Nanoparticle Aggregates in Cancer Cells for Mechanical Destruction with Low Frequency Rotating Magnetic Field

“…Magnetic nanomaterials with remarkable magnetic properties and biocompatibility have been intensively investigated in the field of cancer treatment 1-4. Magnetic nanoparticles (MNPs) are promising nanoplatforms for brain cancer therapy, owing to its capacity to cross blood-brain barrier (BBB) under the remote control of static magnetic field 5-10.…”

Hollow Nanostars with Photothermal Gold Caps and Their Controlled Surface Functionalization for Complementary Therapies

“…The resultant unique starlike Au@SiO 2 with six sharp horns was found to favor cell endocytosis and drug loading. In order to realize efficient gene therapy, low toxic hydroxyl‐rich ethanolamine (EA)‐functionalized poly(glycidyl methacrylate) (PGMA) (BUCT‐PGEA) was proposed to functionalize starlike Au@SiO 2 via surface‐initiated atom transfer radical polymerization (ATRP) to produce starlike Au@SiO 2 ‐PGEA. Thus, the exterior cationic surfaces of the starlike Au@SiO 2 ‐PGEA nanohybrids could be utilized to condense DNA and the interior cavity for anticancer drug loading.…”