Well-defined hydroxyapatite–polycation nanohybrids via surface-initiated atom transfer radical polymerization for biomedical applications

Due to their unique properties, one-dimensional (1D) magnetic nanostructures are of great significance for biorelated applications. A facile and straightforward strategy to fabricate 1D magnetic structure with special shapes is highly desirable. In this work, well-defined peapod-like 1D magnetic nanoparticles (Fe3O4@SiO2, p-FS) are readily synthesized by a facile method without assistance of any templates, magnetic string or magnetic field. There are few reports on 1D gene carriers based on Fe3O4 nanoparticles. BUCT-PGEA (ethanolamine-functionalized poly(glycidyl methacrylate) is subsequently grafted from the surface of p-FS nanoparticles by atom transfer radical polymerization to construct highly efficient gene vectors (p-FS-PGEA) for effective biomedical applications. Peapod-like p-FS nanoparticles were proven to largely improve gene transfection performance compared with ordinary spherical Fe3O4@SiO2 nanoparticles (s-FS). External magnetic field was also utilized to further enhance the transfection efficiency. Moreover, the as-prepared p-FS-PGEA gene carriers could combine the magnetic characteristics of p-FS to well achieve noninvasive magnetic resonance imaging (MRI). We show here novel and multifunctional magnetic nanostructures fabricated for biomedical applications that realized efficient gene delivery and real-time imaging at the same time.

show abstract

“…FS was first modified by APTES to form FS-NH 2 with the terminal amino groups . Then, FS-NH 2 was reacted with BIBB to produce FS-Br.…”

Section: Methodsmentioning

confidence: 99%

“…FS was first modified by APTES to form FS-NH 2 with the terminal amino groups. 29 Then, FS-NH 2 was reacted with BIBB to produce FS-Br. 0.1 g of FS was added in the mixed solvents of 9 mL of ethanol and 1 mL of water.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Well-Defined Peapod-like Magnetic Nanoparticles and Their Controlled Modification for Effective Imaging Guided Gene Therapy

Wang

Zhao

et al. 2016

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…Other polymers such as dextran and polymers derived from pyrrolidine have been reported for DNA as well as RNA delivery . Of these, PDMAEMA is easily modified via copolymerization techniques and relatively good gene transfection efficiencies of various PDMAEMA‐based derivatives have been reported . However, neither PEI nor PDMAEMA are biodegradable, raising concerns on the clearance of the polymer from the body after administration.…”

Section: Introductionmentioning

confidence: 99%

Incorporation of Poly[(R)‐3‐hydroxybutyrate] into Cationic Copolymers Based on Poly(2‐(dimethylamino)ethyl methacrylate) to Improve Gene Delivery

Loh

Ong

Tung

et al. 2013

Macromolecular Bioscience

View full text Add to dashboard Cite

The synthesis and gene transfection efficiency of a series of novel amphiphilic triblock copolymers with two hydrophilic poly(2-(dimethylamino)ethyl methacrylate) (DMAEMA) blocks flanking a central hydrophobic poly[(R)-3-hydroxybutyrate] (PHB) block is reported. These copolymers have significantly lower toxicity compared to the polyethyleneimine (PEI) and PDMAEMA homopolymer. Unexpectedly, the incorporation of PHB significantly improves the gene transfection efficiency as compared to PEI or PDMAEMA homopolymers.

show abstract

“…Microcontact printing (μCP), a versatile method to print patterned self-assembled monolayers (SAMs) by using a poly(dimethylsiloxane) (PDMS) stamp on substrate surfaces, is relatively a more simple, effective, and inexpensive patterning approach for fabricating shaped micro/nanostructures on substrate surfaces . Surface-initiated controlled radical polymerization (SI-CRP) technique − is a widely used method to prepare polymer brushes with controlled grafting densities, functionality, and thicknesses, while surface-initiated atom transfer radical polymerization (SI-ATRP) is one of the most popular SI-CRP methods toward the polymerization of different vinyl monomers with different functional groups for inexpensive initiation systems and mild controllable polymerization conditions. , Therefore, SI-ATRP is no doubt the best partner of μCP to prepare patterned polymer brushes on substrate surfaces.…”

Section: Introductionmentioning

confidence: 99%

“…20 Surface-initiated controlled radical polymerization (SI-CRP) technique 21−24 is a widely used method to prepare polymer brushes with controlled grafting densities, functionality, and thicknesses, while surface-initiated atom transfer radical polymerization (SI-ATRP) is one of the most popular SI-CRP methods toward the polymerization of different vinyl monomers with different functional groups for inexpensive initiation systems and mild controllable polymerization conditions. 25,26 Therefore, SI-ATRP is no doubt the best partner of μCP to prepare patterned polymer brushes on substrate surfaces.…”

Section: ■ Introductionmentioning

confidence: 99%

Noncovalent Microcontact Printing for Grafting Patterned Polymer Brushes on Graphene Films

Gao

Wang

et al. 2013

Langmuir

View full text Add to dashboard Cite

This article describes a simple and universal approach to prepare patterned polymer brushes on graphene-based substrate surfaces by microcontact printing (μCP) of initiator molecules and subsequent surface initiated atom transfer radical polymerization (SI-ATRP) method. Four different initiators are designed and have strong adhesion with graphene-based substrates through noncovalent interaction. Optical and fluorescence microscopy, atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS) were used to characterize the successful polymerization of vinyl monomers on substrate surfaces. To demonstrate the broad applicability of this strategy, polymer brushes with different functionalities including cationic and anionic polyelectrolyte, thermally and pH responsive polymers, as well as polymer patterns on different graphene-based surfaces are fabricated. Binary polymer brushes can also be easily prepared by further initiating the initiator backfilled in the bare areas.

show abstract

Well-defined hydroxyapatite–polycation nanohybrids via surface-initiated atom transfer radical polymerization for biomedical applications

Cited by 13 publications

References 29 publications

Well-Defined Peapod-like Magnetic Nanoparticles and Their Controlled Modification for Effective Imaging Guided Gene Therapy

Well-Defined Peapod-like Magnetic Nanoparticles and Their Controlled Modification for Effective Imaging Guided Gene Therapy

Incorporation of Poly[(R)‐3‐hydroxybutyrate] into Cationic Copolymers Based on Poly(2‐(dimethylamino)ethyl methacrylate) to Improve Gene Delivery

Noncovalent Microcontact Printing for Grafting Patterned Polymer Brushes on Graphene Films

Contact Info

Product

Resources

About