Characterizing the respective nitrogen (N) use efficiency requires understanding the N flow of inputs and outputs from a commercial broiler barn. In this study, an N mass balance was performed for one entire growing cycle. The objectives were to quantify, sample, and analyze all N components entering and leaving the barn. The N from feed, chickens, and bedding material was considered as inputs, the outputs included the N accretion in mature broilers, the total N emissions (N TNE ), the N accumulation in litter, and the N of mortality. Of particular relevance was the determination of an appropriate method to mirror the heterogenic texture of the litter. Litter samples were collected weekly according to a defined procedure. The major N input was feed N, accounting for 99% of the total N input. After the 36-day growing cycle, the N outputs were portioned as follows: 59% (1741.3 kg N) in mature broilers, 37% (1121.3 kg N) accumulated in litter, and 4% in NTNE (114.3 kg N). The N accumulations in broiler tissue and litter agree well with other studies. The measured emissions were consistently lower compared to other references, due to the fact that these references were mainly based on studies where broilers were raised on built-up litter. In contrast to in situ quantified N emissions in this study, other published values were assumed to be the difference of N between inputs and outputs. This study illustrates that extensive sampling of litter is a prerequisite for calculating litter masses. The accurate specification of the litter texture proved to be crucial within the mass balance approach. With this information, the feasible improvements within management practices can be identified.Keywords: ammonia; emissions; mass balance; litter sampling In Europe, the livestock sector strongly influences the nitrogen (N) cycle because large amounts of nutrients, water, and energy are related to the actions of that sector (Steinfeld and Wasenaar, 2007;Erisman et al., 2008). Broiler operations have become larger and more concentrated in recent years due to a growing demand for broiler meat, the production of which is the second largest worldwide (Niu et al., 2009). As a result, the concentration of nutrients in the form of waste products such as manure (litter) and gaseous emissions (Gous, 2010) has increased. The nutrient of the greatest concern in gaseous emissions is nitrogen (N) in the form of ammonia (NH 3 ). Ammonia is formed from the breakdown of nitrogenous waste products in broiler manure (undigested proteins and uric acid) by exogenous enzymes produced by microorganisms (Atapattu et al., 2008).One abatement strategy of NH 3 involves the optimization of dietary composition (protein level) by providing the best feed conversion ratio for broilers and minimizing the excretion of manure (Elwinger and Svensson, 1996;Ferguson et al., 1998;Robertson et al., 2002). Additionally, feeding additives can improve the feed conversion rate of broilers (Ritz et al., 2004;Schiavone et al., 2008). Ammonia is removed from the ...