Weekly Variation of Rotavirus A Concentrations in Sewage and Oysters in Japan, 2014–2016

Ito, Erika; Pu, Jian; Miura, Takayuki; Kazama, Shinobu; Nishiyama, Masateru; Ito, Hiroaki; Konta, Yoshimitsu; Nguyen, Gia Thanh; Omura, Tatsuo; Watanabe, Tôru

doi:10.3390/pathogens8030089

Cited by 8 publications

(4 citation statements)

References 23 publications

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“…RVA was the third most detected virus, with similar or higher detection rates reported in shellfish from Italy, Argentina, Morocco and Japan, ranging from 12.9% to 57.8% [78,[88][89][90]. Compared to our results, other studies detected RVA less frequently in bivalve samples (varying from 0% to 8.3%) in Brazil, Thailand, Italy, South Korea and Singapore [15,18,21,66,74,91].…”

Section: Discussionsupporting

confidence: 81%

Detection and Molecular Characterization of Enteric Viruses in Bivalve Mollusks Collected in Arraial do Cabo, Rio de Janeiro, Brazil

Nascimento

Sarmento

Leonardo

et al. 2022

Viruses

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Viral bivalve contamination is a recognized food safety hazard. Therefore, this study investigated the detection rates, seasonality, quantification, and genetic diversity of enteric viruses in bivalve samples (mussels and oysters). We collected 97 shellfish samples between March 2018 and February 2020. The screening of samples by qPCR or RT-qPCR revealed the detection of norovirus (42.3%), rotavirus A (RVA; 16.5%), human adenovirus (HAdV; 24.7%), and human bocavirus (HBoV; 13.4%). There was no detection of hepatitis A virus. In total, 58.8% of shellfish samples tested positive for one or more viruses, with 42.1% of positive samples contaminated with two or more viruses. Norovirus showed the highest median viral load (3.3 × 106 GC/g), followed by HAdV (median of 3.5 × 104 GC/g), RVA (median of 1.5 × 103 GC/g), and HBoV (median of 1.3 × 103 GC/g). Phylogenetic analysis revealed that norovirus strains belonged to genotype GII.12[P16], RVA to genotype I2, HAdV to types -C2, -C5, and -F40, and HBoV to genotypes -1 and -2. Our results demonstrate the viral contamination of bivalves, emphasizing the need for virological monitoring programs to ensure the quality and safety of shellfish for human consumption and as a valuable surveillance tool to monitor emerging viruses and novel variants.

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Section: Discussionsupporting

confidence: 81%

Detection and Molecular Characterization of Enteric Viruses in Bivalve Mollusks Collected in Arraial do Cabo, Rio de Janeiro, Brazil

Nascimento

Sarmento

Leonardo

et al. 2022

Viruses

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“…RVA was selected as a highly prevalent enteric pathogen, usually found to circulate in the environment ( Ito et al, 2019 ; Steyer et al, 2011 ), in order to additionally evaluate the virus concentration techniques applied for the surface water, wastewater and BMS samples. RVA detection was performed on all samples collected for the present study, by the application of real-time RT-PCR that amplifies the fragment of VP2 segment of different RVA genotypes infecting humans and domestic animals ( Gutiérrez-Aguirre et al, 2008 ).…”

Section: Methodsmentioning

confidence: 99%

SARS-CoV-2 circulation in Croatian wastewaters and the absence of SARS-CoV-2 in bivalve molluscan shellfish

Brnić¹,

Lojkić²,

Škoko³

et al. 2022

Environmental Research

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The circulation of SARS-CoV-2 in the environment has been confirmed numerous times, whilst research on the bioaccumulation in bivalve molluscan shellfish (BMS) has been rather scarce. The present study aimed to fulfil the knowledge gap on SARS-CoV-2 circulation in wastewaters and surface waters in this region and to extend the current knowledge on potential presence of SARS-CoV-2 contamination in BMS. The study included 13 archive wastewater and surface water samples from the start of epidemic and 17 influents and effluents from nine wastewater treatment plants (WWTP) of different capacity and treatment stage, sampled during the second epidemic wave. From that period are the most of 77 collected BMS samples, represented by mussels, oysters and warty venus clams harvested along the Dalmatian coast. All samples were processed according to EN ISO 15216-1 2017 using Mengovirus as a whole process control. SARS-CoV-2 detection was performed by real-time and conventional RT-PCR assays targeting E, N and nsp14 protein genes complemented with nsp14 partial sequencing. Rotavirus A (RVA) real-time RT-PCR assay was implemented as an additional evaluation criterion of virus concentration techniques. The results revealed the circulation of SARS-CoV-2 in nine influents and two secondary treatment effluents from eight WWTPs, while all samples from the start of epidemic (wastewaters, surface waters) were negative which was influenced by sampling strategy. All tertiary effluents and BMS were SARS-CoV-2 negative. The results of RVA amplification were beneficial in evaluating virus concentration techniques and provided insights into RVA dynamics within the environment and community. In conclusion, the results of the present study confirm SARS-CoV-2 circulation in Croatian wastewaters during the second epidemic wave while extending the knowledge on wastewater treatment potential in SARS-CoV-2 removal. Our findings represent a significant contribution to the current state of knowledge that considers BMS of a very low food safety risk regarding SARS-CoV-2.

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“…Even when RVs in the aquatic milieu may be present at an insignicantly low level, concentrating them in this way may potentiate the degree of infectiousness. Human consumption of crustaceans whose basin of production was inltrated by urban contaminants has resulted in several outbreaks of RV gastroenteritis worldwide 46,[51][52][53][54][55] with potentially huge economic costs. Although, bacterial indicators of fecal pollution have been used to reduce the risk of shellsh-associated infections in many countries, their inadequacy for virus detection has necessitated the need to develop new sanitary regulatory strategies based on viral analysis, for improved monitoring standards.…”

Section: Public Health Risks Associated With the Viral Presence In Watermentioning

confidence: 99%

Epidemiological significance of the occurrence and persistence of rotaviruses in water and sewage: a critical review and proposal for routine microbiological monitoring

Omatola

Olaniran

2022

Environ. Sci.: Processes Impacts

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Weekly Variation of Rotavirus A Concentrations in Sewage and Oysters in Japan, 2014–2016

Cited by 8 publications

References 23 publications

Detection and Molecular Characterization of Enteric Viruses in Bivalve Mollusks Collected in Arraial do Cabo, Rio de Janeiro, Brazil

Detection and Molecular Characterization of Enteric Viruses in Bivalve Mollusks Collected in Arraial do Cabo, Rio de Janeiro, Brazil

SARS-CoV-2 circulation in Croatian wastewaters and the absence of SARS-CoV-2 in bivalve molluscan shellfish

Epidemiological significance of the occurrence and persistence of rotaviruses in water and sewage: a critical review and proposal for routine microbiological monitoring

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