INTRODUCTIONWeed seeds represent the main source of annual weed invasion and establishes a weed seed bank in agricultural production and development system (Montaldo, 1995). The weed seed bank reproduces the history of weeds, weeds control and is an indicator of future competition between weeds and crops. The addition of new seeds into the seed bank is affected by how effective the weed control method is. Variations in the weed seed bank are due to direct effect of tillage practices on the existing weed seeds in soil and the plenty of seed producing weeds. Tillage practices influence the weed seed bank where some weed seeds are encouraged to germinate (Roberts and Feast, 1972), although other weed seeds are buried and remain viable in the deep soil for a long period of time (Roberts and Feast, 1972;Mohler and Galford, 1997). Tillage practices work as a filter that influences weed species and weed seed distribution in the soil seed bank. Research show changes in weed species composition as a significance of tillage practices (Cussans, 1975). Different tillage systems can affect the weed species composition of agricultural plant populations varying from traditional tillage to conservation tillage (Ball and Miller, 1993). Dorado et al. (1999) studied the weed seed bank response to crop rotation and tillage i.e. moldboard plow and NT (no-tillage) and they found largest and most diverse weed seed bank in no-tillage system but, according to Wrzesinska et al. (2013) weed species composition and diversity of soil seed bank after ploughing and direct drilling (DD) was same, although in NT treatment this number was lower by three species. Weed control is becoming harder in conservation tillage because of decrease or complete removal of mechanical operations and for this reason, it is important to gain advanced research information about the soil weed seed bank (Forcella et al., Pak. J. Agri. Sci., Vol. 54(4), 907-917; 2017 ISSN (Print) ISSN (Online) Weeds are the major limiting factor for taking higher yields of rain-fed wheat. Temporal and vertical seed bank distribution and weed flora dynamics associated with crop productivity were studied in wheat crop preceding different tillage systems integrated with glyphosate herbicide in a field experiment for two consecutive years. Seven tillage treatments viz. T1: Once Mould board plowing followed by 8 cultivations, T2: No-till followed by twice application of glyphosate, T3: Once Mould board plowing followed by twice application of glyphosate, T4: Once Mould board plowing followed by 4 cultivations, T5: Once disc harrowing followed by twice application of glyphosate, T6: Once disc harrowing followed by 4 cultivations and T7: Once chisel plowing followed by twice application of glyphosate were applied in the fallow periods (summer seasons) of both years. Results showed that seeds of 12 species of winter weeds belonging to nine families were observed in the study area. Seed of five weed species i.e. Chenopodium album, Fumaria indica, Convolvulus arvensis, Asphodelus ten...