Wdr13 and streptozotocin-induced diabetes

Abstract

Type I diabetes, though contributes to only 5–10% of total diabetes cases, is a rising concern in today’s world. Our previous studies have shown that the absence of WDR13 in mouse results in pancreatic β-cell hyper-proliferation. Also, amelioration of the diabetic phenotype on introgression of Wdr13-null (Wdr13-/0) mutation in genetically diabetic mice (Leprdb/db) [type II diabetes] was observed. It was thus, interesting to see the role of WDR13 in streptozotocin-mediated diabetes in mice, a model for type I diabetes. Wdr13-/0 mice along with its wild type (Wdr13+/0 mice) littermates were administered streptozotocin intraperitoneally for 5 consecutive days. Blood glucose levels and body weights of these mice were monitored for subsequent 5 weeks and then they were sacrificed for physiological and histological analyses. Results showed that Wdr13-/0 mice exhibited higher serum insulin levels, better glucose clearance and significantly higher number of proliferating β-cells; reiterating the finding that absence of WDR13 helps in β-cell hyper-proliferation and recovery from diabetes; further underscoring WDR13 as a key target molecule for diabetes treatment/amelioration.

Bioinformatics Analysis of next generation sequencing data for Risk Prediction in Patients with Type 1 diabetes mellitus

“…As a result, 477 up regulated and 383 down regulated genes, at least 4-fold change between T1DM and normal control samples. Wang et al [42], Mishra et al [43], Deaton et al [44] and You et al [45] demonstrated that altered expression of SPHK1, WDR13, GIGYF1 and DNMT3A were found to be substantially related to type 2 diabetes mellitus. Altered expression of SPHK1 [46] was easily found in hypertension.…”

Melatonin Protects Ovary From Diabetes Induced Oxidative Damages in Laboratory Mice.

“…Diabetes was induced by intraperitoneal administration of multiple low doses of streptozotocin (MLD-STZ) to experimental female mice. The STZ was dissolved in freshly prepared 0.1M citrate buffer (pH = 4.5) and was administered (50mg/kg body weight/mice) 10 intraperitoneal for consecutive 5days. .…”

Absence of WDR13 in p53-Null Mice Increases Longevity

“…Earlier studies on WDR13 suggest its involvement in cell cycle regulation in different tissues like pancreas (7)(8)(9), liver (10) adipose (11), colon (12) and uterus (13). Wdr13 expression has also been reported in many human cancers like breast cancer, glioma, Ewing's sarcoma, lung cancer, melanoma, testes cancer, ovary teratocarcinoma, and urothelial cancer (Human protein atlas http://www.proteinatlas.org/ENSG00000101940-WDR13/cancer).…”