Melatonin Protects Ovary From Diabetes Induced Oxidative Damages in Laboratory Mice.

Abstract

Diabetes is a combination of different metabolic disorders as a result of insulin deficiency and improper action. Improper neutralization of reactive oxygen species produced during metabolism results in oxidative stress in living organisms. Melatonin is a known antioxidant, neutralizes reactive oxygen species in living organisms. We have evaluated the effectiveness of low doses (25µg, 50µg and 100µg/100g B.wt.) of melatonin on diabetes caused oxidative damages in the ovary of mice. The induction of diabetes increased the lipid peroxidation (MDA level) and decreased the antioxidant enzyme (SOD and CAT) activity, reduced glutathione (GSH) level, Nrf2 and HO-1 reactivity in the ovary of mice. Melatonin supplementation suppressed the MDA level and increased the SOD, and CAT activity, GSH levels, Nrf2 and HO-1 reactivity in the ovary of experimental mice in a dose-dependent pattern. Therefore, the present study may suggest that melatonin ameliorates the adverse effects of diabetes by reducing the oxidative stress in the ovary of experimental mice.