Waving away from the poison: recovery of the nuclear structure after chemotropic response in roots against poison

Kurup, Aditya; Aiswarya, K S; Manoj, Bejoy; Rafeeka, Rinsa Salahudeen; Singh, Mayanglambam Suheshkumar

doi:10.1117/12.2609961

Cited by 2 publications

(2 citation statements)

References 16 publications

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“…Selective plane illumination microscopy (SPIM) or light sheet fluorescence microscopy (LSFM) is a fast non-destructive imaging modality that has great application in live and 3D imaging with very low photo-toxicity and photobleaching. [7][8][9] Along with these benefits sMx-SPIM provides the imaging of biological specimens at different magnifications and resolutions at the same time. In biological research, fluorescence microscopy has been widely used for imaging.…”

Section: Introductionmentioning

confidence: 99%

A study on mitochondrial dynamics and membrane potential in mammalian cells using home-built simultaneous multiple-level magnification selective plane illumination microscopy (sMx-SPIM)

K.S.,

Narendradev,

Narumbam

et al. 2024

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXII

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Mitochondria are vital organelles responsible for energy production and they undergo dynamic morphological changes influenced by the cell’s metabolic state. Mitochondrial membrane potential is an essential component in the process of energy storage in the cells. The membrane potential plays a crucial role in the functioning of mitochondria, and it can influence mitochondrial velocity through various mechanisms. Here we report correlation studies on the mitochondrial dynamics and mitochondrial membrane potential in mammalian cells using our home-built sMx-SPIM imaging system. The mitochondria are tracked manually by a self-developed algorithm to find the speed. The speed of mitochondria was determined by targeting mitochondria stained with MitoTracker Deep Red CMXRos dye and measurement of mitochondrial membrane potential was done by tetramethylrhodamine methyl ester (TMRM). Experimental studies were conducted on mammalian cells in alive conditions, and results are provided.

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Section: Introductionmentioning

confidence: 99%

A study on mitochondrial dynamics and membrane potential in mammalian cells using home-built simultaneous multiple-level magnification selective plane illumination microscopy (sMx-SPIM)

K.S.,

Narendradev,

Narumbam

et al. 2024

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXII

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“…3 Selective plane illumination microscopy (SPIM) or light sheet fluorescence microscopy (LSFM) is a versatile and virtually non-destructive imaging tool that combines optical sectioning with multiple-view imaging and provides great penetration depth, minimal photo-toxicity, and photo-bleaching, and many more benefits over the confocal microscope. 4,5 These advantages make it better for 3D and live imaging for wide spatiotemporal range. 6 Hence the integration of LSFM with multispectral imaging provides a significant advantage in bioimaging.…”

Section: Introductionmentioning

confidence: 99%

Multi-spectral imaging in mammalian cells using a home-built LSFM system

K.S.¹,

S.R.²,

Sharma³

et al. 2023

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI

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Light sheet fluorescence microscopy (LSFM) works on fluorescence imaging, employing target-specific fluorescent proteins to label regions of interest in a sample. In this way, analyzing and manipulating information from different targets in a specimen requires labeling the specimen using multiple fluorophores. Multispectral imaging helps identify several target molecules simultaneously at once, thereby retrieving information from the entire volume of the sample. Here we report multispectral imaging studies on mammalian cells using our in-house built multispectral LSFM imaging system. Experimental studies were conducted on mammalian cells in alive conditions, and results are provided.

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Waving away from the poison: recovery of the nuclear structure after chemotropic response in roots against poison

Cited by 2 publications

References 16 publications

A study on mitochondrial dynamics and membrane potential in mammalian cells using home-built simultaneous multiple-level magnification selective plane illumination microscopy (sMx-SPIM)

A study on mitochondrial dynamics and membrane potential in mammalian cells using home-built simultaneous multiple-level magnification selective plane illumination microscopy (sMx-SPIM)

Multi-spectral imaging in mammalian cells using a home-built LSFM system

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