Wavelength distinguishable signal quenching and enhancing toward photoactive material 3,4,9,10-perylenetetracarboxylic dianhydride for simultaneous assay of dual metal ions

Huang, Liaojing; Deng, Hanmei; Zhong, Xia; Zhu, Minghui; Yuan, Ruo; Yuan, Yali

doi:10.1016/j.bios.2019.111702

Cited by 19 publications

(7 citation statements)

References 34 publications

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“…The Au NPs deposited electrochemically on the PTCDA@C 60 /ITO electrode further increases the photocurrent of the electrode due to the good electron transfer capability of Au NPs (curve c). When S0-MB is immobilized on the Au NPs/PTCDA@C 60 /ITO electrode via the Au–S bond, the photocurrent increases obviously due to the approaching of the sensitizer (MB) to the PTCDA@C 60 composite. , Further modification of MCH weakens the photocurrent signal slightly due to the blocking effect of MCH (curve e). After this, the hybridization between S0-MB and Ab1-S1-Fc makes the photocurrent decrease sharply due to the weakening of the enhancing effect of MB and the strengthening of the inhibiting effect of Fc on the Au NPs/PTCDA@C 60 /ITO electrode (curve f).…”

Section: Resultsmentioning

confidence: 99%

Sensitive Dual-Mode Biosensors for CYFRA21-1 Assay Based on the Dual-Signaling Electrochemical Ratiometric Strategy and “On–Off–On” PEC Method

Zhang

Xiao

et al. 2021

Anal. Chem.

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Herein, an electrochemical (EC)–photoelectrochemical (PEC) dual-mode biosensor was constructed for cytokeratin 19 fragment 21-1 (CYFRA21-1) assay based on the dual-signaling electrochemical ratiometric strategy and “on–off–on” PEC method. The indium tin oxide (ITO) electrode was modified by 3,4,9,10-perylenetetracarboxylic dianhydride (PTCDA)@C60 and gold nanoparticles (Au NPs), and the double-stranded DNA composed of thiol/methylene blue (MB)-labeled single-stranded DNA (ssDNA) (S0-MB) and antibody/ferrocene (Fc)-labeled ssDNA (Ab1-S1-Fc) was immobilized on the Au NPs/PTCDA@C60/ITO electrode via the Au–S bond between Au NPs and thiol of S0-MB. With the help of another antibody-labeled ssDNA (Ab2-S2), the presence of CYFRA21-1 triggered a typical antigen–antibody sandwich immune reaction (Ab1, CYFRA21-1, and Ab2) and proximity hybridization between Ab1-S1-Fc and Ab2-S2. This caused the release of Ab1-S1-Fc from the modified electrode and the change of S0-MB to a hairpin structure, resulting in a decrease (an increase) of the oxidation peak current of Fc (MB) and an increase of the photocurrent due to the enhancing (inhibiting) effect of MB (Fc) on the photoelectric performance of the Au NPs/PTCDA@C60/ITO electrode. Thus, CYFRA21-1 was detected by the developed EC–PEC dual-mode sensing platform sensitively, and the linear response ranges of 0.001–40 ng/mL with a detection limit of 0.3 pg/mL for the EC technique and 0.0001–4 ng/mL with a detection limit of 0.03 pg/mL for the PEC method were obtained. Furthermore, by changing the specific antibodies of disease-related biomarkers, the developed dual-mode biosensing platform could be readily extended to detect other antigens, implying its great potential applications in biological analysis and early disease diagnosis.

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Section: Resultsmentioning

confidence: 99%

Sensitive Dual-Mode Biosensors for CYFRA21-1 Assay Based on the Dual-Signaling Electrochemical Ratiometric Strategy and “On–Off–On” PEC Method

Zhang

Xiao

et al. 2021

Anal. Chem.

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“…Almost all of the PEC sensors for Pb 2+ detection were designed based on DNA sensing. [69][70][71][72][73][74][75][76][77] Zhao et al used porphyrin-based covalent organic framework thin films as cathodic materials, and the steric hindrance effect was generated by using CdSe@SiO2 as the quenching agent to construct an On-Off-On type PEC sensor for Pb 2+ detection (Fig. 3C).…”

Section: Heavy Metalsmentioning

confidence: 99%

Research progress on photoelectrochemical sensors for contamination analysis in agricultural fields

et al. 2022

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The establishment of rapid and efficient contamination detection methods in agricultural fields is of great significance for safeguarding agricultural production and human health, and for ensuring the healthy development of agricultural industries. As a booming analytical method, photoelectrochemical (PEC) sensors, which combine the advantages of electrochemical analysis and optical analysis, have received increasing research attention. Due to the complete separation of the excitation source (light) and the detection signal (current), the background signal of PEC sensors is greatly decreased, and their sensitivity is high. This review aims to cover the advances in PEC sensors for contamination analysis in agricultural fields over the past five years, including analysis of heavy metals, antibiotics, pesticides, and toxins. The future prospects in this field are also discussed.

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“…After the electrodes are modified with VS2 (curve b) and AuNPs/VS2 (curve c), the Rct value continues to decrease to 132 Ω and 67 Ω, respectively, proving the good conductivity of nanostructures. When arch DNA, MCH and cruciform DNA are added to the electrode surface in turn (curves d, e and f), the Rct value increases greatly to 810 Ω, 960 Ω, and 1432 Ω, respectively, since both negatively charged DNA strands and MCH can reduce the electron transfer efficiency and increase the resistance of the system [35]. Finally, with the further immobilization of target cell MCF-7 and biotin-tagged HRP on the electrode surface (curve g and h), the Rct value increases to 1710 Ω and 2053 Ω.…”

Section: Electrochemical Characterizationmentioning

confidence: 99%

Aptamer Embedded Arch-Cruciform DNA Assemblies on 2-D VS2 Scaffolds for Sensitive Detection of Breast Cancer Cells

et al. 2021

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Arch-cruciform DNA are self-assembled on AuNPs/VS2 scaffold as a highly sensitive and selective electrochemical biosensor for michigan cancer foundation-7 (MCF-7) breast cancer cells. In the construction, arch DNA is formed using two single-strand DNA sequences embedded with the aptamer for MCF-7 cells. In the absence of MCF-7 cells, a cruciform DNA labeled with three terminal biotin is bound to the top of arch DNA, which further combines with streptavidin-labeled horseradish peroxidase (HRP) to catalyze the hydroquinone-H2O2 reaction on the electrode surface. The presence of MCF-7 cells can release the cruciform DNA and reduce the amount of immobilized HRP, thus effectively inhibiting enzyme-mediated electrocatalysis. The electrochemical response of the sensor is negatively correlated with the concentration of MCF-7 cells, with a linear range of 10 − 1 × 105 cells/mL, and a limit of detection as low as 5 cells/mL (S/N = 3). Through two-dimensional materials and enzyme-based dual signal amplification, this biosensor may pave new ways for the highly sensitive detection of tumor cells in real samples.

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Wavelength distinguishable signal quenching and enhancing toward photoactive material 3,4,9,10-perylenetetracarboxylic dianhydride for simultaneous assay of dual metal ions

Cited by 19 publications

References 34 publications

Sensitive Dual-Mode Biosensors for CYFRA21-1 Assay Based on the Dual-Signaling Electrochemical Ratiometric Strategy and “On–Off–On” PEC Method

Sensitive Dual-Mode Biosensors for CYFRA21-1 Assay Based on the Dual-Signaling Electrochemical Ratiometric Strategy and “On–Off–On” PEC Method

Research progress on photoelectrochemical sensors for contamination analysis in agricultural fields

Aptamer Embedded Arch-Cruciform DNA Assemblies on 2-D VS2 Scaffolds for Sensitive Detection of Breast Cancer Cells

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