Wavelength-coded volume holographic imaging endoscope for multidepth imaging

Howlett, Isela D.; Han, Wanglei; Rice, Photini F. S.; Barton, Jennifer K.; Kostuk, Raymond K.

doi:10.1117/1.jbo.22.10.100501

Cited by 3 publications

(1 citation statement)

References 22 publications

(27 reference statements)

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“…Standard wide‐field endoscopy provides wide field‐of‐view (FoV) and three‐dimensional (3D) information of tissue samples; however, wide‐field endoscopy has poor optical sectioning ability to reject out‐of‐focus background, which limits its medical use for imaging thick biological samples . Many recent improvements in optical fluorescence endoscopy have development of optical sectioning capabilities, particularly centered on increasing acquisition speed, and enhancing image quality of resolution and contrast .…”

Section: Introductionmentioning

confidence: 99%

Speckle illumination holographic non‐scanning fluorescence endoscopy

Lin

Singh

et al. 2018

Journal of Biophotonics

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Optical sectioning endoscopy such as confocal endoscopy offers capabilities to obtain three-dimensional (3D) information from various biological samples by discriminating between the desired in-focus signals and out-of-focus background. However, in general confocal images are formed through point-by-point scanning and the scanning time is proportional to the 3D space-bandwidth product. Recently, structured illumination endoscopy has been utilized for optically sectioned wide-field imaging, but it still needs axial scanning to acquire images from different depths of focal plane. Here, we report wide-field, multiplane, optical sectioning endoscopic imaging, incorporating 3D active speckle-based illumination and multiplexed volume holographic gratings, to simultaneously obtain images of fluorescently labeled tissue structures from different depths, without the need of scanning. We present the design, and implementation, as well as experimental data, demonstrating this endoscopic system's ability to obtain optically sectioned multiplane fluorescent images of tissue samples, with cellular level resolution in wide-field fashion, and no need for mechanical or optical axial scanning.(A) Schematic drawing of the SIHN endoscopy to simultaneously acquire multiplane images from different depths. (B) Uniform, and (C) SIHN illuminated images of standard fluorescence beads (25 μm in diameter) for the two axial planes. (D) Intensity profile on fluorescently labeled signal (ie, in-focus) and background (ie, out-of-focus) of microspheres.

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Section: Introductionmentioning

confidence: 99%

Speckle illumination holographic non‐scanning fluorescence endoscopy

Lin

Singh

et al. 2018

Journal of Biophotonics

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Simultaneous multi-color optical sectioning fluorescence microscopy with wavelength-coded volume holographic gratings

et al. 2020

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Optical sectioning fluorescence microscopy provides high contrast images of volumetric samples and has been widely used for many biological applications. However, simultaneously acquiring multi-color fluorescence images require additional optical elements and devices, which are bulky, wavelength specific, and not cost-effective. In this paper, wavelength-coded volume holographic gratings (WC-VHGs) based optical sectioning fluorescence microscopy is proposed to simultaneously offer multi-color fluorescence images with fine out-of-focus background rejection. Due to wavelength degeneracy, multiplexed WC-VHGs are capable of acquiring multi-wavelength fluorescence images in a single shot, and displaying the laterally separated multi-wavelength images onto CCD. In our system optical sectioning capability is achieved through speckle illumination and HiLo imaging method. To demonstrate imaging characteristics of our system, dual-wavelength fluorescence images of both standard fluorescent microspheres and ex vivo mT/mG mice cardiac tissue are presented. Current results may find important applications in hyperspectral imaging for biomedical research.

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Advanced volume holographic filter to improve the SNR of polychromatic sources in a noisy environment

Alcaraz

Blanche

2021

Opt. Express

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We present a new type of filter that improves the SNR of systems where polychromatic signal and noise are located at different distances within the same line of sight. The filter is based on holographic technology that allows for the discrimination of wavefronts by range. In using a combination of two holographic elements, a pre-disperser and a thick volume hologram, we were able to significantly increase the spectral bandwidth of the filter, from 9nm without the pre-disperser to 70nm with both holographic elements. Laboratory proof of concept demonstrated that such a filter is capable of an SNR improvement of 15 dB for a monochromatic source, and up to 7.6 dB for a polychromatic source. This filter can find applications in astronomic observation, satellite or space debris tracking, and free-space optical communication.

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Wavelength-coded volume holographic imaging endoscope for multidepth imaging

Cited by 3 publications

References 22 publications

Speckle illumination holographic non‐scanning fluorescence endoscopy

Speckle illumination holographic non‐scanning fluorescence endoscopy

Simultaneous multi-color optical sectioning fluorescence microscopy with wavelength-coded volume holographic gratings

Advanced volume holographic filter to improve the SNR of polychromatic sources in a noisy environment

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