Water state in chemically and physically crosslinked chitosan membranes

Ostrowska-Czubenko, Jadwiga; Pieróg, Milena; Gierszewska, Magdalena

doi:10.1002/app.39357

Cited by 44 publications

(46 citation statements)

References 40 publications

(71 reference statements)

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“…As we have discussed earlier [6], the band at 1648 cm −1 is most probably composed of the amide I band of chitosan and the C=N stretching band of a Schiff base. Schiff bases, depending on the compound, absorb in the range 1620-1660 cm −1 [9].…”

Section: Resultssupporting

confidence: 52%

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pH-responsive hydrogel membranes based on modified chitosan: water transport and kinetics of swelling

2015

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pH-responsive membranes prepared by treating chitosan (Ch) with glutaraldehyde (GA) or with GA and sulfuric acid (SA) were studied. The structure and properties of the membranes were characterized by Fourier transform infrared (FTIR) spectroscopy, wide-angle X-ray diffraction (WAXD), scanning electron microscopy (SEM), atomic force microscopy (AFM), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and elemental analysis. The effect of the cross-linking process both on the dynamic swelling behaviour of the hydrogel membranes and on the mechanism of water transport through those membranes was investigated in buffer solutions of different pH (1.2-9.5). The mechanism of water transport through the hydrogel chitosan membranes was affected by membrane composition and pH of the swelling medium. Non-cross-linked Ch membrane showed a non-Fickian swelling behaviour in the pH range 6.5-9.5. Chitosan membrane cross-linked with GA (Ch/GA) showed less-Fickian or Fickian swelling behaviour in all buffer solutions. In the case of chitosan membrane cross-linked with GA and SA (Ch/GA/SA), at low pH (lower than the pK a of the hydrogel) the water transport was controlled more by polymer relaxation than by penetrant diffusion. The experimental data clearly suggested that the swelling process in all buffer solutions obeyed second-order kinetics. Values of an apparent swelling rate constant for Ch/ GA and Ch/GA/SA membranes were of the same order of magnitude for acidic and neutral swelling media but they increased for alkaline solutions.

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Section: Resultssupporting

confidence: 52%

“…The viscosity average molecular weight (M v ) of chitosan solutions determined by viscosity analysis was 730±16 kDa. The details of DDA and M v determinations are described elsewhere [6].…”

Section: Chitosan Characterizationmentioning

confidence: 99%

pH-responsive hydrogel membranes based on modified chitosan: water transport and kinetics of swelling

2015

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“…The droplets are crosslinked between positively charged amino groups of chitosan present in nHA/chitosan mixture and negatively charged ion of TPP. This ionic crosslink formation can be confirmed by the translation in antisymmetric deformation N-H vibrations in NH 3+ in chitosan at 1589 cm −1 to a lower wave number of present at 1558 cm −1 on all composite spectrums [42-44]. …”

Section: Discussionmentioning

confidence: 80%

Injectable porous nano-hydroxyapatite/chitosan/tripolyphosphate scaffolds with improved compressive strength for bone regeneration

Uswatta

Okeke

Jayasuriya

2016

Materials Science and Engineering: C

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In this study we have fabricated porous injectable spherical scaffolds using chitosan biopolymer, sodium tripolyphosphate (TPP) and nano-hydroxyapatite (nHA). TPP was primarily used as an ionic crosslinker to crosslink nHA/chitosan droplets. We hypothesized that incorporating nHA into chitosan could support osteoconduction by emulating the mineralized cortical bone structure, and improve the Ultimate Compressive Strength (UCS) of the scaffolds. We prepared chitosan solutions with 0.5%, 1% and 2% (w/v) nHA concentration and used simple coacervation and lyophilization techniques to obtain spherical scaffolds. Lyophilized spherical scaffolds had a mean diameter of 1.33 mm (n=25). Further, portion from each group lyophilized scaffolds were soaked and dried to obtain Lyophilized Soaked and Dried (LSD) scaffolds. LSD scaffolds had a mean diameter of 0.93 mm (n=25) which is promising property for the injectability. Scanning Electron Microscopy images showed porous surface morphology and interconnected pore structures inside the scaffolds. Lyophilized and LSD scaffolds had surface pores less than 10 and 2 µm, respectively. 2% nHA/chitosan LSD scaffolds exhibited UCS of 8.59 MPa compared to UCS of 2% nHA/chitosan lyophilized scaffolds at 3.93 MPa. Standardize UCS values were 79.98 MPa and 357 MPa for 2% nHA/chitosan lyophilized and LSD particles respectively. One-way ANOVA results showed a significant increase (p < 0.001) in UCS of 1% and 2% nHA/chitosan lyophilized scaffolds compared to 0% and 0.5% nHA/chitosan lyophilized scaffolds. Moreover, 2% nHA LSD scaffolds had significantly increased (p < 0.005) their mean UCS by 120% compared to 2% nHA lyophilized scaffolds. In a drawback, all scaffolds have lost their mechanical properties by 95% on the 2nd day when fully immersed in phosphate buffered saline. Additionally live and dead cell assay showed no cytotoxicity and excellent osteoblast attachment to both lyophilized and LSD scaffolds at the end of 14th day of in vitro studies. 2% nHA/chitosan scaffolds showed higher osteoblast attachment than 0% nHA/chitosan scaffolds.

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“…The water state of the hydrogels was evaluated using a DSC Q2000 (TA-Instruments, New Castle, DE) according to the procedure proposed by Ostrowska-Czubenko et al 18 Seven Tzero pans were loaded with approximately 5 mg of freeze-dried samples. The water state of the hydrogels was evaluated using a DSC Q2000 (TA-Instruments, New Castle, DE) according to the procedure proposed by Ostrowska-Czubenko et al 18 Seven Tzero pans were loaded with approximately 5 mg of freeze-dried samples.…”

Section: Hydrogels Preparationmentioning

confidence: 99%

“…18 The following equation was used to calculate the freezing water (W f ): The melting temperature and the melting enthalpy (ΔH m ) were calculated using the Universal Analysis 2000 software (version 4.7a; TA Instruments, Newcastle, DE).…”

Section: Hydrogels Preparationmentioning

confidence: 99%

Development of gelatin/chitosan/PVA hydrogels: Thermal stability, water state, viscoelasticity, and cytotoxicity assays

Rodríguez‐Rodríguez

García-Carvajal

Jiménez‐Palomar³

et al. 2018

J of Applied Polymer Sci

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Gelatin/chitosan/poly(vinyl alcohol) (PVA) hydrogels were fabricated with different polymer ratio using the freeze-drying process. The thermal stability, water state, rheological, and cytotoxic properties of the hydrogels were evaluated. Thermogravimetric/differential scanning calorimetry analyses showed a decomposition onset temperature below 242.7 AE 2.7 C. The samples did not show statistical differences (p < 0.05) on the onset temperature values. Nonfreezing water reached a constant value around of 1 g water/g polymer. Freezing water increased linearly with the increase of the water content independently of the polymer ratio. The hydrogels showed an equilibrium water content from 9 to 13 times their mass. The hydrogels exhibited a solid-viscoelastic behavior. The elastic modulus was higher with the increase of chitosan concentration (G 0 = 22 170 AE 85 Pa) independently of the temperature (5-55 C). In vitro assay showed that hydrogels are nontoxic in the HT29-MTX-E12 cell line. These results indicate that the gelatin/chitosan/PVA hydrogels could be considered for biomedical applications.

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Water state in chemically and physically crosslinked chitosan membranes

Cited by 44 publications

References 40 publications

pH-responsive hydrogel membranes based on modified chitosan: water transport and kinetics of swelling

pH-responsive hydrogel membranes based on modified chitosan: water transport and kinetics of swelling

Injectable porous nano-hydroxyapatite/chitosan/tripolyphosphate scaffolds with improved compressive strength for bone regeneration

Development of gelatin/chitosan/PVA hydrogels: Thermal stability, water state, viscoelasticity, and cytotoxicity assays

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