Water‐stable electrospun collagen fibers from a non‐toxic solvent and crosslinking system

Jiang, Qiuran; Reddy, Narendra; Zhang, Simeng; Roscioli, Nicholas; Yang, Yiqi

doi:10.1002/jbm.a.34422

Cited by 97 publications

(75 citation statements)

References 42 publications

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“…Typically, volatile organic solvents are used for electrospinning, which can alter the conformation of the dissolved collagen peptides [108] and prevent reassembly into fibrils that exhibit the native staggered configuration and distinctive banding pattern. Such malformed fibers are not stable in water and will dissolve over time [109, 112]. In addition, the toxicity and high salt content of the solvents conventionally used in electrospinning have hampered its use in cell-contacting situations.…”

Section: 0 - Isolation and Reconstitution Of Collagen Into Hydrogelmentioning

confidence: 99%

“…In addition, the toxicity and high salt content of the solvents conventionally used in electrospinning have hampered its use in cell-contacting situations. However, more recent efforts have resulted in the ability to electrospin collagen from relatively benign solvents, such as mixtures of ethanol and water [110], and crosslinking strategies have been developed to enhance the stability of even very thin fibers [111, 112]. These resulting fiber meshes are not hydrogels themselves, however, they are typically hydrated for use with cells, and the resulting matrices resemble fibrillar hydrogels.…”

Section: 0 - Isolation and Reconstitution Of Collagen Into Hydrogelmentioning

confidence: 99%

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Strategies for directing the structure and function of three-dimensional collagen biomaterials across length scales

2014

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Collagen type I is a widely used natural biomaterial that has found utility in a variety of biological and medical applications. Its well characterized structure and role as an extracellular matrix protein make it a highly relevant material for controlling cell function and mimicking tissue properties. Collagen type I is abundant in a number of tissues, and can be isolated as a purified protein. This review focuses on hydrogel biomaterials made by reconstituting collagen type I from a solubilized form, with an emphasis on in vitro studies in which collagen structure can be controlled. The hierarchical structure of collagen from the nanoscale to the macroscale is described, with an emphasis on how structure is related to function across scales. Methods of reconstituting collagen into hydrogel materials are presented, including molding of macroscopic constructs, creation of microscale modules, and electrospinning of nanoscale fibers. The modification of collagen biomaterials to achieve desired structures and functions is also addressed, with particular emphasis on mechanical control of collagen structure, creation of collagen composite materials, and crosslinking of collagenous matrices. Biomaterials scientists have made remarkable progress in rationally designing collagen-based biomaterials and in applying them to both the study of biology and for therapeutic benefit. This broad review illustrates recent examples of techniques used to control collagen structure, and to thereby direct its biological and mechanical functions.

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Section: 0 - Isolation and Reconstitution Of Collagen Into Hydrogelmentioning

confidence: 99%

Section: 0 - Isolation and Reconstitution Of Collagen Into Hydrogelmentioning

confidence: 99%

Strategies for directing the structure and function of three-dimensional collagen biomaterials across length scales

2014

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“…However, the highly hydrated nature of conventional collagen gels renders them structurally weak and difficult to manipulate 22 . Several approaches have been taken to overcome the mechanical and geometrical drawbacks of collagen-based hydrogels 23–25 .…”

Section: Introductionmentioning

confidence: 99%

Tissue-engineered cornea constructed with compressed collagen and laser-perforated electrospun mat

Kong

Sun

Chen

et al. 2017

Sci Rep

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While Plastic Compressed (PC) collagen technique is often used to fabricate bioengineered constructs, PC collagen gels are too weak to be sutured or conveniently handled for clinical applications. To overcome this limitation, electrospun poly (lactic-co-glycolide) (PLGA) mats, which have excellent biocompatibility and mechanical properties, were combined with PC collagen to fabricate sandwich-like hybrid constructs. By laser-perforating holes with different sizes and spacings in the electrospun mats to regulate the mechanical properties and light transmittance of the hybrid constructs, we produced hybrid constructs with properties very suitable to apply in corneal tissue engineering. The maximum tensile stress of the optimal hybrid construct was 3.42 ± 0.22 MPa. The light transmittance of the hybrid construct after perforation was approximately 15-fold higher than before, and light transmittance increased gradually with increasing time. After immersing into PBS for 7 days, the transmittance of the optimal construct changed from 63 ± 2.17% to 72 ± 1.8% under 500 nm wavelength. The live/dead staining, cell proliferation assay and immunohistochemistry study of human corneal epithelial cells (HCECs) and human keratocytes (HKs) cultured on the optimal hybrid construct both demonstrated that the cells adhered, proliferated, and maintained their phenotype well on the material. In addition, after culturing for 2 weeks, the HCECs could form stratified layers. Thus, our designed construct is suitable for the construction of engineered corneal tissue.

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“…The improvement was limited since the bers substantially swelled aer incubated in PBS for 15 days. 6,7 Another common protein for tissue engineering, silk broin lacked surface cell-binding portions and necessitated surface functionalization to facilitate cell attachment and proliferation.…”

Section: Introductionmentioning

confidence: 99%

Intrinsically water-stable electrospun three-dimensional ultrafine fibrous soy protein scaffolds for soft tissue engineering using adipose derived mesenchymal stem cells

Cai

Sellers

et al. 2014

RSC Adv.

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Intrinsically water-stable electrospun threedimensional ultrafine fibrous soy protein scaffolds for soft tissue engineering using adipose derived mesenchymal stem cells" (2014 Soy protein, the plant protein from soybean, was electrospun into intrinsically water-stable scaffolds with large volume and ultrafine fibers oriented randomly and evenly in three dimensions (3D) to simulate native extracellular matrices of soft tissues. The 3D ultrafine fibrous scaffolds from proteins could be favored in soft tissue engineering. However, protein-based biomaterials usually suffered from poor water stability, while the highly crosslinked proteins which had water stability were usually difficult to be fabricated into fibers. Soy protein was a typical protein with intrinsic water stability, attributed to its 1.2% cysteine content. Soy protein has been developed into 3D non-fibrous structures, coarse fibers and films for tissue engineering applications, but not ultrafine fibrous structures. In this research, the disulfide crosslinks in soy protein were cleaved to facilitate its dissolution in an aqueous solvent system. The obtained solution was electrospun into bulky scaffolds composed of ultrafine fibers oriented randomly in three dimensions. Without external crosslinking, the fibrous soy protein scaffolds demonstrated longterm water stability, and maintained their fibrous structures after incubated in PBS for up to 28 days. In vitro study showed that the 3D soy protein scaffolds well supported uniform distribution and adipogenic differentiation of adipose derived mesenchymal stem cells. In summary, the 3D ultrafine fibrous soy protein structures could be good candidates as scaffolds in soft tissue engineering.

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Water‐stable electrospun collagen fibers from a non‐toxic solvent and crosslinking system

Cited by 97 publications

References 42 publications

Strategies for directing the structure and function of three-dimensional collagen biomaterials across length scales

Strategies for directing the structure and function of three-dimensional collagen biomaterials across length scales

Tissue-engineered cornea constructed with compressed collagen and laser-perforated electrospun mat

Intrinsically water-stable electrospun three-dimensional ultrafine fibrous soy protein scaffolds for soft tissue engineering using adipose derived mesenchymal stem cells

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