Strategies for directing the structure and function of three-dimensional collagen biomaterials across length scales

Walters, Brandan; Stegemann, Jan P.

doi:10.1016/j.actbio.2013.08.038

Cited by 190 publications

(154 citation statements)

References 226 publications

(257 reference statements)

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“…Collagen is often used as an excellent matrix for cell attachment due to its integrin binding sites [89]. The threedimensional porous structure of collagen hydrogels allows sufficient exchange of oxygen, wastes and nutrients inside the scaffold [90] for short-term or long-term MCS culture [91].…”

Section: Natural Polymersmentioning

confidence: 99%

Advances in multicellular spheroids formation

Cui¹,

Hartanto²,

Zhang³

2017

J. R. Soc. Interface.

394

336

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Three-dimensional multicellular spheroids (MCSs) have a complex architectural structure, dynamic cell -cell/cell -matrix interactions and bio-mimicking in vivo microenvironment. As a fundamental building block for tissue reconstruction, MCSs have emerged as a powerful tool to narrow down the gap between the in vitro and in vivo model. In this review paper, we discussed the structure and biology of MCSs and detailed fabricating methods. Among these methods, the approach in microfluidics with hydrogel support for MCS formation is promising because it allows essential cell -cell/cell -matrix interactions in a confined space.

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Section: Natural Polymersmentioning

confidence: 99%

Advances in multicellular spheroids formation

Cui¹,

Hartanto²,

Zhang³

2017

J. R. Soc. Interface.

394

336

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“…12,38 Acid solubilization is most commonly used for minimally crosslinked collagens, such as rat tail tendon, while a combination of neutral salt solution with proteolytic extraction (pepsin digestion) is often used in order to fully denature highly crosslinked collagens from bovine or porcine skin. 11,20,39 Many combinations of collagen sources, solubilization techniques, and solvents are in use for preparation of collagen stock solutions. Further, collagen stock solutions, even those produced commercially, have been found to have significant batch-to-batch variation.…”

Section: Collagen Source and Solubilizationmentioning

confidence: 99%

Review of Collagen I Hydrogels for Bioengineered Tissue Microenvironments: Characterization of Mechanics, Structure, and Transport

Antoine

Vlachos

Rylander³

2014

Tissue Engineering Part B: Reviews

464

353

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Type I collagen hydrogels have been used successfully as three-dimensional substrates for cell culture and have shown promise as scaffolds for engineered tissues and tumors. A critical step in the development of collagen hydrogels as viable tissue mimics is quantitative characterization of hydrogel properties and their correlation with fabrication parameters, which enables hydrogels to be tuned to match specific tissues or fulfill engineering requirements. A significant body of work has been devoted to characterization of collagen I hydrogels; however, due to the breadth of materials and techniques used for characterization, published data are often disjoint and hence their utility to the community is reduced. This review aims to determine the parameter space covered by existing data and identify key gaps in the literature so that future characterization and use of collagen I hydrogels for research can be most efficiently conducted. This review is divided into three sections: (1) relevant fabrication parameters are introduced and several of the most popular methods of controlling and regulating them are described, (2) hydrogel properties most relevant for tissue engineering are presented and discussed along with their characterization techniques, (3) the state of collagen I hydrogel characterization is recapitulated and future directions are proposed. Ultimately, this review can serve as a resource for selection of fabrication parameters and material characterization methodologies in order to increase the usefulness of future collagenhydrogel-based characterization studies and tissue engineering experiments.

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“…A quitosana, é um exemplo desse tipo de material, sendo muito utilizado para a fabricação de scaffolds na reparação de tecidos [1,4,5]. A quitosana é um copolímero composto por N-acetil-2-amino-2-desoxi-D-glucopiranose e 2-amino-2-desoxi-D-glucopiranose, onde os dois tipos de unidades de repetição estão ligadas por (1→4)-β-glicosídica, estas unidades estão distribuídas em diferentes graus, dependendo das porções acetiladas.…”

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Avaliação do método de obtenção de scaffolds quitosana/curcumina sobre a estrutura, morfologia e propriedades térmicas

et al. 2016

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RESUMOPesquisas tem sido realizada com objetivo de combinar propriedades dos materiais com a finalidade de melhorar o processo de regeneração. Portanto scaffolds como sistemas de liberação controlada de fármacos tem sido estudado a fim de proporcionar o crescimento do tecido ao mesmo tempo que a região esteja sendo tratada. Diante disso, essa pesquisa tem como objetivo desenvolver scaffolds de quitosana e avaliar a inserção do fármaco curcumina por meio dos métodos de reação de solução e adsorção visando obter um sistema quitosana/curcumina para liberação controlada do fármaco. Os scaffolds foram produzidos pelo método de agregação de esferas, onde as esferas foram inicialmente obtidas a partir do gotejamento da solução de quitosana e quitosana/curcumina em solução de sulfato de sódio (5%) e posteriormente agregadas pela solução de gelatina (5%). Foram realizadas as caracterizações de espectroscopia no infravermelho com transformada de Fourier (FTIR), microscopia ótica (MO), microscopia eletrônica de varredura (MEV) e calorimetria exploratória diferencial (DSC). Por FTIR sugeriu-se a interação da quitosana com a curcumina. A partir das avaliações morfológicas por MO e MEV foi possível identificar o fármaco no interior das estruturas pelos dois métodos utilizado. E por DSC observou-se para amostra de quitosana/curcumina o desaparecimento do pico de fusão característico da curcumina, indicando assim que a curcumina foi encapsulada na quitosana no estado amorfo, o que provavelmente ocasionará uma liberação mais lenta do fármaco. Dessa forma, constatou-se que as estruturas obtidas são promissoras para o estudo de liberação controlada de fármaco.Palavras-chave: Curcumina, Quitosana, Scaffolds. ____________________________________________________________________________________ ABSTRACTResearches have been conducted in order to match material properties to improve the regeneration process. Thereby, scaffolds as controlled drug delivery systems have been studied in order to enhance the obtained structure by providing tissue growth while the region is being treated. Thus, this research aims to develop scaffolds of chitosan, and avaluatinh the insertion of curcumin drug by the methods of , solutions reaction and adsorption, aiming at obtaining a chitosan/curcumin for controlled drug release system. The scaffolds were produced by beads aggregation method, in which the beads were originally obtained from the dropping of chitosan solution and chitosan / curcumin in sodium sulphate solution (5%) and then attached by the gelatin solution (5%). The following characterizations were performed: Fourier Transform Infrared Spectroscopy (FTIR), Optical Microscopy (OM), Scanning Electron Microscopy (SEM) and Differential Scanning Calorimetry (DSC). By FTIR analysis, it was possible to verify the likely interaction of chitosan with curcumin. From the morphological analysis by OM and SEM it was possible to identify the drug inside the structures by both methods. DSC analysis showed the total disappearance of the characteristic melting ...

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Strategies for directing the structure and function of three-dimensional collagen biomaterials across length scales

Cited by 190 publications

References 226 publications

Advances in multicellular spheroids formation

Advances in multicellular spheroids formation

Review of Collagen I Hydrogels for Bioengineered Tissue Microenvironments: Characterization of Mechanics, Structure, and Transport

Avaliação do método de obtenção de scaffolds quitosana/curcumina sobre a estrutura, morfologia e propriedades térmicas

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