Water-Soluble Non-Ionic PEGylated Porphyrins: A Versatile Category of Dyes for Basic Science and Applications

Villari, Valentina; Micali, N.; Nicosia, Angelo; Mineo, Placido

doi:10.1007/s41061-021-00348-4

Cited by 6 publications

(6 citation statements)

References 92 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the tested Star3600_Me systems (Me = nickel, zinc, tin, manganese, rhodium, copper, erbium, mercury, iron), only those containing Zn, Sn, Hg, Rh and Er ions can generate singlet oxygen species (the respective quantum yields are reported in Table 1 ). The inability of some metal to produce singlet oxygen (nickel, tin, manganese, copper, erbium, iron) porphyrins could be due to the static quenching phenomenon [ 30 ], hindering the attainment of the excited state [ 44 ].…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, the presence of charges in the porphyrin structure could determine the denaturation of proteins and/or hinder the penetration of the porphyrin itself inside cells, aside from the non-specific binding that can lead the porphyrin to be bound to proteins or biomolecules with complementary charges rather than the target protein [ 29 ]. An alternative viable option is the use of PEGylated porphyrins [ 30 ], where the presence of PEG chains in peripheral positions with an appropriate polymerization degree [ 30 , 31 ] renders the porphyrin suitable for biomedical applications (avoiding the aggregation phenomena), guaranteeing excellent water solubility, stealth behaviors towards the immune system and biocompatibility [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Water-Soluble Star Polymer as a Potential Photoactivated Nanotool for Lysozyme Degradation

Mezzina,

Nicosia,

Barone

et al. 2024

Polymers

Self Cite

View full text Add to dashboard Cite

The development of nanotools for chemical sensing and macromolecular modifications is a new challenge in the biomedical field, with emphasis on artificial peptidases designed to cleave peptide bonds at specific sites. In this landscape, metal porphyrins are attractive due to their ability to form stable complexes with amino acids and to generate reactive oxygen species when irradiated by light of appropriate wavelengths. The issues of hydrophobic behavior and aggregation in aqueous environments of porphyrins can be solved by using its PEGylated derivatives. This work proposes the design of an artificial photo-protease agent based on a PEGylated mercury porphyrin, able to form a stable complex with l-Tryptophan, an amino acid present also in the lysozyme structure (a well-known protein model). The sensing and photodegradation features of PEGylated mercury porphyrin were exploited to detect and degrade both l-Trp and lysozyme using ROS, generated under green (532 nm) and red (650 nm) light lasers. The obtained system (Star3600_Hg) and its behavior as a photo-protease agent were studied by means of several spectroscopies (UV-Vis, fluorescence and circular dichroism), and MALDI-TOF mass spectrometry, showing the cleavage of lysozyme and the appearance of several short-chain residues. The approach of this study paves the way for potential applications in theranostics and targeted bio-medical therapies.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Water-Soluble Star Polymer as a Potential Photoactivated Nanotool for Lysozyme Degradation

Mezzina,

Nicosia,

Barone

et al. 2024

Polymers

Self Cite

View full text Add to dashboard Cite

show abstract

“…The UV-Vis spectrum of PNH 2 ( Figure 4 a) shows the Soret band at λ = 418 nm, and the Q-bands at about 517, 557, 592 and 652 nm. According to Gouterman’s four orbitals model [ 81 , 82 ], the Soret band originates from the S 0 -S 2 electronic transition, while the Q-bands are due to the S 0 -S 1 electronic transitions [ 48 ].…”

Section: Resultsmentioning

confidence: 99%

“…[ 42 ], transition metal complexes [ 43 ], phthalocyanines [ 44 ] and porphyrinoids [ 45 , 46 ]. In particular, porphyrinoids are well-known for their high molar extinction coefficients in the visible range and their light-harvesting ability [ 47 , 48 ]. Their versatility is exploited in multi-faceted applications [ 48 ], such as for the preparation of sensors [ 49 , 50 , 51 , 52 , 53 ] or theranostic agents [ 54 , 55 , 56 , 57 ].…”

Section: Introductionmentioning

confidence: 99%

Photosensitized Thermoplastic Nano-Photocatalysts Active in the Visible Light Range for Potential Applications Inside Extraterrestrial Facilities

et al. 2022

Self Cite

View full text Add to dashboard Cite

Among different depollution methods, photocatalysis activated by solar light is promising for terrestrial outdoor applications. However, its use in underground structures and/or microgravity environments (e.g., extraterrestrial structures) is forbidden. In these cases, there are issues related to the energy emitted from the indoor lighting system because it is not high enough to promote the photocatalytic mechanism. Moreover, microgravity does not allow the recovery of the photocatalytic slurry from the depolluted solution. In this work, the synthesis of a filmable nanocomposite based on semiconductor nanoparticles supported by photosensitized copolyacrylates was performed through a bulk in situ radical copolymerization involving a photosensitizer macromonomer. The macromonomer and the nanocomposites were characterized through UV-Vis, fluorescence and NMR spectroscopies, gel permeation chromatography and thermogravimetric analysis. The photocatalytic activity of the sensitized nanocomposites was studied through photodegradation tests of common dyes and recalcitrant xenobiotic pollutants, employing UV-Vis and visible range (λ > 390 nm) light radiations. The sensitized nanocomposite photocatalytic performances increased about two times that of the unsensitized nanocomposite and that of visible range light radiation alone (>390 nm). The experimental data have shown that these new systems, applied as thin films, have the potential for use in indoor deep underground and extraterrestrial structures.

show abstract

“…Imaging techniques are another fundamental aspect of biomedical treatments because they enable the visualisation and localisation of diseased tissues and allow physicians to follow the progress during treatment. Various fluorescent probes are commonly used for imaging tumours, including tetraphenylethylene [ 38 ], porphyrin [ 13 , 39 , 40 ], fluorescein [ 41 ], and rhodamine B [ 42 ].…”

Section: Introductionmentioning

confidence: 99%

A Multifunctional Conjugated Polymer Developed as an Efficient System for Differentiation of SH-SY5Y Tumour Cells

et al. 2022

View full text Add to dashboard Cite

Polymer-based systems have been demonstrated in novel therapeutic and diagnostic (theranostic) treatments for cancer and other diseases. Polymers provide a useful scaffold to develop multifunctional nanosystems that combine various beneficial properties such as drug delivery, bioavailability, and photosensitivity. For example, to provide passive tumour targeting of small drug molecules, polymers have been used to modify and functionalise the surface of water-insoluble drugs. This approach also allows the reduction of adverse side effects, such as retinoids. However, multifunctional polymer conjugates containing several moieties with distinct features have not been investigated in depth. This report describes the development of a one-pot approach to produce a novel multifunctional polymer conjugate. As a proof of concept, we synthesised polyvinyl alcohol (PVA) covalently conjugated with rhodamine B (a tracking agent), folic acid (a targeting agent), and all-trans retinoic acid (ATRA, a drug). The obtained polymer (PVA@RhodFR) was characterised by MALDI-TOF mass spectrometry, gel permeation chromatography, thermal analysis, dynamic light-scattering, NMR, UV-Vis, and fluorescence spectroscopy. Finally, to evaluate the efficiency of the multifunctional polymer conjugate, cellular differentiation treatments were performed on the neuroblastoma SH-SY5Y cell line. In comparison with standard ATRA-based conditions used to promote cell differentiation, the results revealed the high capability of the new PVA@RhodFR to induce neuroblastoma cells differentiation, even with a short incubation time and low ATRA concentration.

show abstract

Water-Soluble Non-Ionic PEGylated Porphyrins: A Versatile Category of Dyes for Basic Science and Applications

Cited by 6 publications

References 92 publications

Water-Soluble Star Polymer as a Potential Photoactivated Nanotool for Lysozyme Degradation

Water-Soluble Star Polymer as a Potential Photoactivated Nanotool for Lysozyme Degradation

Photosensitized Thermoplastic Nano-Photocatalysts Active in the Visible Light Range for Potential Applications Inside Extraterrestrial Facilities

A Multifunctional Conjugated Polymer Developed as an Efficient System for Differentiation of SH-SY5Y Tumour Cells

Contact Info

Product

Resources

About