Water-Soluble and Biocompatible MnO@PVP Nanoparticles for MR Imaging <I>In Vitro</I> and <I>In Vivo</I>

Abstract

The uniform-sized manganese oxide nanoparticles (the oleic-capped MnO NPs) were synthesized by the thermal decomposition of Mn-oleate complex and were transferred into water with the help of cationic surfactant of cetyltrimethyl ammonium bromide (CTAB), then the poly(vinylpyrrolidone) (PVP) membrane was further coated on to them with the aid of anionic dispersant of poly(styrenesulfonate) (PSS) by layer-by-layer electrostatic assembly to render them water soluble and biocompatible. They were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), X-ray diffraction (XRD), Fourier-transform infrared (FTIR) and MTT assay. In vitro cellular uptake test revealed the MnO@PVP NPs were low cytotoxic, biocompatible and could be used as a T,-positive contrast agent for passive targeting magnetic resonance imaging (MRI). Interestingly, signal enhancement in cerebral spinal fluid (CSF) spaces in vivo experiment suggested that the MnO@PVP NPs can pass through the blood brain barrier (BBB). These results show that MnO@PVP NPs are good candidates as MRI contrast agents with the lack of cytotoxicity and have great potential applications in magnetic nano-device and biomagnetic field.

Facile synthesis of perovskite CeMnO3 nanofibers as an anode material for high performance lithium-ion batteries

“…The peaks at 520 and 433 cm À1 refer to the metal-oxygen stretching vibrations of Ce-O and Mn-O. 40,43 The SEM images of Ce(NO 3 ) 3 -MnAc/PVP NFs and CeMnO 3 NFs are shown in Fig. 3.…”

Applications and Biological Activity of Nanoparticles of Manganese and Manganese Oxides in In Vitro and In Vivo Models

“…The relaxivity value specifies the ability to contrast the image by brightening specific tissues; therefore, agents with higher relaxivity can be administered at a lower dose, which increases their safety. Another nanomaterial, MnO NPs coated with polyvinylpyrrolidone (PVP), turned out to have good contrasting properties in MRI, enhancing the brightness in a dose-dependent manner [ 24 ]. Mn 3 O 4 NPs modified with polyethylene glycol (PEG) and fluorescent dye from the cyanine group (Cy 7.5 ), developed by Zhan et al [ 25 ], allowed a dual-modality imaging based on magnetic resonance and fluorescence.…”

“…MnO NPs functionalised with PVP, designed as an MRI contrasting agent, were investigated for their uptake and cytotoxicity. After a 24-h treatment of human lung carcinoma cells (SPCA-1), no differences in viability between treated and control cells were observed [ 24 ]. Cytotoxicity study, performed by Bi et al [ 38 ] as a part of new theranostic tool development, showed no cytotoxic effect of MnO 2 nanosheets on L929 fibroblast cells.…”

<p>Manganese Oxide Nanoparticles As MRI Contrast Agents In Tumor Multimodal Imaging And Therapy</p>

“…In particular, MnO@PVP NPs can pass through the blood–brain barrier (BBB) and gradually metabolize to other sites with blood flow. This is indicated as an intravascular MRI CA (r 1 =1.937 mM −1 s −1 , r 2 =27.879 mM −1 s −1 , 3.0 T) and a potential application in basic neuroscience research 35. Hsu et al encapsulated MONPs with silica-F127 (PEO 106 PPO 70 PEO 106 ) in order to make them highly hydrophilic.…”