In June of 2015, we investigated the distribution of biogenic elements, heavy metals, and pesticides in the waters of the Shardara Reservoir. The average content of ammonium, nitrates, nitrites and phosphates in the water did not exceed the limit values set for the fishery water bodies. Zinc concentration in the water averaged 0.121, copper -0.040, cadmium -0.003, lead -0.034 mgdm -3 , isomers of hexachlorcyclohexane (HCCH) -0.0041 mg dm -3 . Wafer plots were built in order to identify the distribution correlation of hydro-chemical and toxic variables with the sources of pollution in Shardara reservoir, and statistical methods were used as well (correlation, multiple regression analysis methods, canonical correspondence analysis, and ecological mapping with the Statistica 12.0 Program). It was found that copper, nitrates, and isomers of HCCH enter the reservoir mainly with the drainage water discharged from irrigated fields along the southern bank. The source of mineral phosphorus, polyphosphates, nitrites and zinc is the river runs off rom the east. High concentration of heavy metals in the water could also be related to natural causes, as the water reservoir and its tributaries are located in a metallogenic province.