Water Permeability of an Ovarian Antral Follicle Is Predominantly Transcellular and Mediated by Aquaporins

McConnell, Nisha A.

doi:10.1210/en.143.8.2905

Cited by 43 publications

(45 citation statements)

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“…AQPs have not been extensively studied in the ovary thus far, and this study provides the first evidence of AQP1 and AQP5 expression in ovarian tissues. The AQP9 expression has been previously shown in rat granulosa cells (McConnell et al 2002); however, we showed the subcellular localization of AQP9 within the granulosa cells. In turn, Edashige et al (2000) showed the mRNA expression of AQP3 and AQP7 in mouse oocytes.…”

Section: Aqps In Ovarycontrasting

confidence: 53%

“…This is why the transport of water in this organ seems to be an important process for the effective reproduction. Recently, AQP7, 8, and/or 9 have been shown to participate in water influx across the ovarian follicle wall primarily through transcellular transport mechanisms in the rat (McConnell et al 2002). Importantly, AQP9 is the member of aquaglyceroporin subgroup, which is permeable not only to water but also to glycerol, urea, and other non-electrolytes.…”

Section: Aqps In Ovarymentioning

confidence: 99%

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Immunolocalization of Aquaporin 1, 5, and 9 in the Female Pig Reproductive System

Skowroński

Kwon

Nielsen

2008

J Histochem Cytochem.

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(SN) S U M M A R Y Thirteen mammalian aquaporin (AQP) isoforms have been identified, and they have a unique tissue-specific pattern of expression. AQPs have been documented in the reproductive system of both male and female humans, rats, and mice. However, tissue expression and cellular and subcellular localization of AQPs are unknown in the female reproductive system of pigs. In this study, AQP1 immunoreactivity was detected in the capillary endothelium of the ovary. Distinct immunolabeling of capillary endothelium was also observed in the oviduct and uterus. AQP5 was expressed in flattened follicle cells of primordial follicles, granulosa cells of developing ovarian follicles, and muscle cells of the oviduct and uterus. Staining of AQP5 was also observed in the epithelial cells of the oviduct and uterine epithelium. AQP9 immunoreactivity was observed in granulosa cells of developing follicles. AQP9 was also localized in the luminal epithelial cells of the oviduct and uterine epithelia cells. This is, to our knowledge, the first study that shows tissue expression and cellular and subcellular localization of AQPs in the reproductive system of the female pig. Moreover, these results suggest that several subtypes of the AQPs (AQP1, 5, and 9) are involved in regulation of water homeostasis in the reproductive system of gilts. The first confirmation of AQP in the female reproductive system was obtained by isolating and sequencing the cDNA encoding a water channel from the human uterus (Li et al. 1994). Afterward, Li et al. (1997) found AQP1 mRNA in the rat uterus. To date, based on protein expression, at least nine AQP isoforms have been shown to be expressed in the female reproductive system of humans, rats, and mice (reviewed by Huang et al. 2006). Their specific expression pattern suggests that they play a role in water movement between the intraluminal, interstitial, and capillary compartments. There is also evidence indicating that ovarian steroid can regulate the expression of several AQPs (Jablonski et al. 2003;Branes et al. 2005;Lindsay and Murphy 2006). Nevertheless, data concerning the expression and role of AQPs in female reproductive tissues are still very limited and not available in relation to farm animals.

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Section: Aqps In Ovarycontrasting

confidence: 53%

Section: Aqps In Ovarymentioning

confidence: 99%

Immunolocalization of Aquaporin 1, 5, and 9 in the Female Pig Reproductive System

Skowroński

Kwon

Nielsen

2008

J Histochem Cytochem.

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“…Subsequent studies have identified more than 10 different types of AQPs in mammals, found in several organs of different species, such as human kidney (Deen et al, 1994), rat epithelial tissue (Lindsay and Murphy, 2007), human blood vessels (Agre and Kozono, 2003), reproductive system of rodents (McConnell et al, 2002), dogs (Domeniconi et al, 2007), and pigs (Skowronski et al, 2009). The studies with AQP1 demonstrated that these proteins are small and integral membrane protein types, crossing the membrane from side to side, and containing two semipores that together form a water channel (Verkman and Mitra, 2000), assisting the transport of solutes (Thoroddsen et al, 2011).…”

Section: Aquaporinsmentioning

confidence: 99%

Review Structure, function, and localization of aquaporins: their possible implications on gamete cryopreservation

Sales¹,

Lobo²,

Carvalho³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. The discovery of water channels (aquaporins, AQPs) was a landmark event for the clarification of water transport through the plasma membrane. AQPs belong to a family of intrinsic membrane proteins that act as selective channels for water and for solutes such as glycerol and urea. AQPs were found in different tissues and organs, including male and female reproductive systems. In the swine female reproductive system, the AQPs were localized in the uterus, oviduct, and ovary, as well as in the granulosa cells from primordial follicles. Knowing the involvement of AQPs with the male and female germ cells, as well as their acknowledged role in transporting water through the plasma membrane, the research of these proteins in cryopreservation processes becomes essential. Thus, this review aims to describe the structure and function of AQPs in membranes, highlighting their role 6719 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 12 (4): 6718-6732 (2013) Overview and role of aquaporins on gamete cryopreservation in the reproductive system (male and female). We also discuss the involvement of AQPs in cryopreservation, focusing on the effect and importance of these proteins on the rates of vitrification protocols for preantral follicles present in the ovarian tissue of domestic mammals.

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“…17,[22][23][24][25][26] Thirteen mammalian AQP isoforms have been identified in the reproductive tract of human and rodent females. [26][27][28][29][30][31][32][33] AQP2 not only acts as an exclusive water channel that is tightly regulated by estrogen in the ovariectomized mouse endometrium treated with E2, but also augments water permeability. 26) However, the presence of an estrogen and/or progesterone response element in the AQP2 promoter has remained obscure.…”

Section: Discussionmentioning

confidence: 99%

Estrogenic Effects of a Kampo Formula, <i>Tokishakuyakusan</i>, in Parous Ovariectomized Rats

Chung

Suzuki

Nishihara

et al. 2008

Biological & Pharmaceutical Bulletin

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Tokishakuyakusan (TS; Dang-Gui-Shao-Yao-San in Chinese) is a popular prescription in traditional Chinese (referred to as Kampo in Japan) medicine. The Ministry of Health, Labour and Welfare in Japan, has approved TS for treating diseases of women such as menopausal syndrome, 1) dysmenorrhea,2) luteal insufficiency, 3) amenorrhea, 4) chill 5) and many other disorders. TS recovers the hypothalamic pituitary adenylate cyclase-activating polypeptide (PACAP) that regulates the synthesis and secretion of gonadotropin 6) and PACAP type I receptor expression in hypophysectomized and ovariectomized rats, 7) maturates ovarian follicles in hypophysectomized rats, 8) promotes ovarian estrogen and progesterone production and secretion, 9,10) and stimulates cAMP accumulation. 11)Hormone replacement therapy (HRT) has recently been used to treat menopausal syndrome because estrogen suppresses climacteric complaints, but the hazards of HRT are now better understood. The Women's Health Initiative Study was abandoned because of several adverse effects including the higher risk for hormone dependent cancer and coronary heart disease outweighed the benefits of hormone treatment for postmenopausal women. 12,13) The incidence of carcinoma of the uterine cervix in parous women who are under HRT is high, because exogenous estrogen targets the uterus.14) Although the estrogenic action of TS is accepted in clinical experiments and studies in vitro, the mechanism of TS and possible risks of uterine carcinoma remain unclear. The present study investigates the effects of TS on the uterus of bilaterally ovariectomized parous rats. MATERIALS AND METHODS AnimalsFemale parous Wistar rats (250-300 g, Japan SLC Inc., Shizuoka, Japan) were bilaterally ovariectomized (OVX) or sham-operated (Sham), acclimatized for 7 d and then maintained in a light, temperature and humidity controlled environment (lights on, 07:00-19:00 h, 22Ϯ2°C and 50Ϯ5%, respectively) with access to food and water ad libitum. The animals were handled in accordance with the Guide for Animal Care Committee of the University of Toyama.Preparation of TS and Regents The Kampo formula, TS, comprised six crude drugs (Table 1), all of which were purchased from Tochimoto Tenkaido (Osaka, Japan). The mixture (24 g) of crude drugs for TS that represents one daily dose was extracted by boiling in 1 l of water for 1 h. The decoction was filtered, concentrated under reduced pressure, and lyophilized to yield 4.296 g of powdered extract. A portion of the extract was then dissolved in tap water (400 mg/ml) immediately before oral administration. We purchased naringinase and 17b-estradiol (E2) from SigmaAldrich Japan Co. (Tokyo, Japan), o-nitrophenyl b-D-galactopyranoside (ONPG) from Nacalai Tesque (Kyoto, Japan) and antibodies from the sources shown in parentheses.Experimental Schedule The parous sham rats (nϭ4) were given water [10 ml/kg body weight, peros (p.o.)] once each day for 2 weeks. The parous OVX rats were separated into 5 groups of 4 animals each and administered with drugs once each ...

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Water Permeability of an Ovarian Antral Follicle Is Predominantly Transcellular and Mediated by Aquaporins

Cited by 43 publications

References 0 publications

Immunolocalization of Aquaporin 1, 5, and 9 in the Female Pig Reproductive System

Immunolocalization of Aquaporin 1, 5, and 9 in the Female Pig Reproductive System

Review Structure, function, and localization of aquaporins: their possible implications on gamete cryopreservation

Estrogenic Effects of a Kampo Formula, <i>Tokishakuyakusan</i>, in Parous Ovariectomized Rats

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